1989
DOI: 10.1152/ajpgi.1989.256.4.g645
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Identification of the intestinal Na-phosphate cotransporter

Abstract: Na-dependent phosphate uptake in intestinal brush-border membrane vesicles was sensitive to arginine group-specific reagents in a substrate-sensitive manner. Four different arginine group-specific reagents were tested. All four reagents irreversibly inhibited Na-dependent phosphate uptake with the concentration for 50% inhibition, K0.5, varying between 150 and 40 microM. Maximum inhibition approached 80%. Addition of substrates during exposure to these reagents resulted in protection of Na-phosphate cotranspor… Show more

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Cited by 7 publications
(15 citation statements)
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“…Brush-border membrane vesicles were prepared by Ca 2. precipitation of intestinal mucosal scrapings as previously described [15,20]. Following isolation, brush-border membrane vesicles were resuspended in 300 mM mannitol + 10 mM HEPES/Tris, pH 7.5, and stored at liquid N 2 temperature until needed.…”
Section: Preparation Of Brush-border Membrane Vesiclesmentioning
confidence: 99%
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“…Brush-border membrane vesicles were prepared by Ca 2. precipitation of intestinal mucosal scrapings as previously described [15,20]. Following isolation, brush-border membrane vesicles were resuspended in 300 mM mannitol + 10 mM HEPES/Tris, pH 7.5, and stored at liquid N 2 temperature until needed.…”
Section: Preparation Of Brush-border Membrane Vesiclesmentioning
confidence: 99%
“…The CaZ+-precipitated brush-border membrane vesicles were further purified using 0.02% SDS and a sucrose step gradient centrifugation procedure, as previously described [14]. Briefly, Ca2+-precipitated brush-border membrane vesicles were treated with 0.02% SDS for 15 rain in the presence of 100 mM Na +, 10 mM glucose, 10 mM proline and 50 mM potassium phosphate, pH 7.4, at a final protein concentration of 2 mg/ml.…”
Section: Preparation Of Brush-border Membrane Vesiclesmentioning
confidence: 99%
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