2007
DOI: 10.1074/jbc.m610540200
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Identification of the Intracellular Region of the Leukotriene B4 Receptor Type 1 That Is Specifically Involved in Gi Activation

Abstract: . The i3-1 mutant, with a mutation at the i3 amino terminus, exhibited greatly reduced GTP␥S binding but intact inositol phosphate accumulation triggered by leukotriene B 4 stimulation. These results suggest that the i3-1 region is required only for G i activation. Moreover, in the i3-1 mutant, the deficiency in G i activation was accompanied by a loss of the high affinity leukotriene B 4 binding state seen with the wild type receptor. A three-dimensional model of BLT1 constructed based on the structure of bov… Show more

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Cited by 39 publications
(41 citation statements)
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“…To our knowledge, LTB4R expression on human ASM cells has not been previously identified. In recombinant expression systems, the receptor has been shown to couple to both G ␣i and G ␣q (22). Given that G ␣q -coupled receptors act to constrict ASM, this suggests that LTB 4 could evoke bronchoconstriction directly, rather than via leukocyte recruitment.…”
Section: Discussionmentioning
confidence: 99%
“…To our knowledge, LTB4R expression on human ASM cells has not been previously identified. In recombinant expression systems, the receptor has been shown to couple to both G ␣i and G ␣q (22). Given that G ␣q -coupled receptors act to constrict ASM, this suggests that LTB 4 could evoke bronchoconstriction directly, rather than via leukocyte recruitment.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, reduced phagocytosis in BLT1-deficient BMM was most likely not due to alternation in Syk phosphorylation. Rac is intimately involved in macrophage phagocytosis and functions downstream of G i -coupled G-protein-coupled receptors, including BLT1 (29). LTB 4 activated Rac in WT BMM but not in BLT1-deficient BMM (Fig.…”
Section: Blt1 Is Required For Macrophage Phagocytosis Via Fc␥rs-mentioning
confidence: 94%
“…Within tissues, chemoattractants also direct the encounter between immune cells, thereby playing a regulatory role in immune homeostasis. Most chemoattractants, including chemokines, complement C5a, fMLP, leukotriene B 4 , and others (5)(6)(7)(8), signal through G protein-coupled receptors (GPCRs) that mediate their functions mainly via the heterotrimeric G i -proteins (9). Ligand binding to this class of GPCRs triggers Ga i subunits to exchange GTP for GDP, resulting in the dissociation of the Ga i subunit from Gbg heterodimers.…”
mentioning
confidence: 99%