IDENTIFICATION OF THE Lanr1 GENE OF RESISTANCE TO ANTHRACNOSE OF NARROW-LEAFED LUPINE (Lupinus angustifolius L.) USING DNA-MARKERS AnSeq3 AND AnSeq4

Grishin, Sergei Y.; Zayakin, V. V.; Nam, In-Gil; Агеева, П А; Лукашевич, М. И.; Kuptsov, N. S.

doi:10.15389/agrobiology.2015.1.30eng

Cited by 2 publications

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“…Our study revealed very low frequency of the Lanr1 allele in tested germplasm panel (about 6%). This observation converges with the result of Eastern European germplasm screening with Anseq3 and Anseq4 markers, which evidenced the presence of Lanr1 allele only in two Belarusian lines 40 . It indicates that Lanr1 allele has not been widely exploited by local breeding programs, contrary to Australia, where it was one of the key alleles targeted by marker-assisted selection 30 .…”

Section: Discussionsupporting

confidence: 89%

A successful defense of the narrow-leafed lupin against anthracnose involves quick and orchestrated reprogramming of oxidation–reduction, photosynthesis and pathogenesis-related genes

Książkiewicz

Rychel-Bielska

Plewiński

et al. 2022

Sci Rep

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Narrow-leafed lupin (NLL, Lupinus angustifolius L.) is a legume plant cultivated for grain production and soil improvement. Worldwide expansion of NLL as a crop attracted various pathogenic fungi, including Colletotrichum lupini causing a devastating disease, anthracnose. Two alleles conferring improved resistance, Lanr1 and AnMan, were exploited in NLL breeding, however, underlying molecular mechanisms remained unknown. In this study, European NLL germplasm was screened with Lanr1 and AnMan markers. Inoculation tests in controlled environment confirmed effectiveness of both resistance donors. Representative resistant and susceptible lines were subjected to differential gene expression profiling. Resistance to anthracnose was associated with overrepresentation of “GO:0006952 defense response”, “GO:0055114 oxidation–reduction process” and “GO:0015979 photosynthesis” gene ontology terms. Moreover, the Lanr1 (83A:476) line revealed massive transcriptomic reprogramming quickly after inoculation, whereas other lines showed such a response delayed by about 42 h. Defense response was associated with upregulation of TIR-NBS, CC-NBS-LRR and NBS-LRR genes, pathogenesis-related 10 proteins, lipid transfer proteins, glucan endo-1,3-beta-glucosidases, glycine-rich cell wall proteins and genes from reactive oxygen species pathway. Early response of 83A:476, including orchestrated downregulation of photosynthesis-related genes, coincided with the successful defense during fungus biotrophic growth phase, indicating effector-triggered immunity. Mandelup response was delayed and resembled general horizontal resistance.

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Section: Discussionsupporting

confidence: 89%