Identification of the LEA family members from Caragana korshinskii (Fabaceae) and functional characterization of CkLEA2-3 in response to abiotic stress in Arabidopsis

Abstract

Plants are exposed to different abiotic stresses that affect growth, development, and productivity. Previous studies have shown that late embryogenesis abundant (LEA) proteins play important roles in plant adaptation to abiotic stresses. However, reports that demonstrate their biological functions, especially in Caragana korshinskii Kom., are still very limited. In this study, 26 LEA genes were identified from dehydration-treated suppressive subtractive hybridizations cDNA library and transcriptome sequencing data of C. korshinskii, and were classified into seven groups according to their structural features. Quantitative real-time PCR analysis revealed that the CkLEAs were induced by abscisic acid (ABA) and diverse abiotic stresses and widely expressed in various tissues. Moreover, overexpression of CkLEA2-3 in Arabidopsis thaliana L. Heynh resulted in enhanced tolerance to ABA treatment, and osmotic and salt stresses during seed germination. CkLEA2-3 overexpression lines also exhibited resistance to drought stress during seedling development. Taken together, our results indicate that CkLEA2-3 plays positive roles in conferring abiotic stress tolerance in Arabidopsis.

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

“…Moreover, in comparison with the YS form and the wild type, the transgenic plants with K-segment constructs conserved higher catalase and peroxide dismutase enzymatic activity and maintained lower levels of malondialdehyde and H 2 O 2 [119]. Moreover, in a study, the overexpression of a Caragana korshinskii (Fabaceae) group II LEA gene, CkLEA2-3, in Arabidopsis thaliana, led to enhanced protection to osmotic stress under the seed germination stage [120].…”

“…2019 Arabidopsis Drought [24] Oryza sativa OsDhnRab16 Rice Drought [146] Capsicum annuum CaDHN5 Arabidopsis Salt and osmotic [125] Korshinsk pea shrub CkLEA2-3 Arabidopsis Salt and osmotic [120] African lily ApY2SK2 and ApSK3 Arabidopsis Salt, osmotic, cold, and drought [121] Zea mays ZmDHN13 Yeast and tobacco Oxidative stress [147] 9.…”

Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar

“…For example, transcription factors and protein kinases can regulate downstream signal transduction pathways, which eventually lead to physiological responses to the stresses [ 3 ]. On the other hand, functional proteins in plants, such as the late embryogenesis abundant (LEA) proteins can eliminate cellular content of active oxygen species, in order to protect macromolecular substances and alleviate damages caused by the abiotic stresses [ 4 ].…”

Quaternary Ammonium Salts of Iminofullerenes: Fabrication and Effect on Seed Germination

“…They were highly hydrophilic and protected cells from water stress by capturing enough water into cells . Yu and colleagues reported that CkLea2-3 overexpression lines had a higher tolerance to osmotic stress during seed germination . The upregulated LEA protein (spot 4) in IFQA-treated samples was similar with the SBP65 protein, which is a LEA-like protein and involved in a protective role during seed germination .…”