Identification of the lipids and the ant attractant 1,2-dioleoylglycerol in the arils of Commiphora guillaumini Perr. (Burseraceae) by supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry

Schmeer, Karl; Nicholson, Graeme; Zhang, Shigang; Bayer, Ernst; Böhning‐Gaese, Katrin

doi:10.1016/0021-9673(95)01118-8

Cited by 14 publications

(10 citation statements)

References 16 publications

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“…The report described a lab-built open tubular column SFC/MS interface to a commercial APCI ionization source on a Sciex API III tandem quadrupole mass spectrometer. Several years later, Schmeer et al (33) reported the analysis of lipids from the seed arils of Commiphora guillaumini, a tree species native to western Madagascar. TAG and DAG were separated using SFC and identified by APCI-MS. In-source fragmentation caused by orifice voltages greater than 80 V was used to provide structural information.…”

Section: Sfcmentioning

confidence: 99%

Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids

Byrdwell

2001

Lipids

247

134

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Atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) has proven to be a very valuable technique for analysis of lipids from a variety of classes. This instrumental method readily produces useful ions with gentle fragmentation from large neutral molecules such as triacylglycerols and carotenoids, which are often difficult to analyze using other techniques. Molecules that are easily ionized, such as phospholipids, produce molecular ions and diagnostically useful fragment ions that are complementary to those produced by methods such as electrospray ionization MS with collision-induced dissociation. The simplicity and versatility of APCI-MS make it an ideal tool for use in solving hitherto very difficult analytical problems.

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Section: Sfcmentioning

confidence: 99%

Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids

Byrdwell

2001

Lipids

247

134

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“…All these applications involved the installation of an additional heating source at the SFC sample outlet. One of the reports described glycerolipids in arils (23), another the ionization of triacylglycerols with one reference compound as an example (19). In general, no detailed studies concerning SFC-MS of triacylglycerols have been published.…”

mentioning

confidence: 97%

“…Some articles concerning applications for both packed and capillary column SFC-(APCI)MS have been published and deal with the analysis of such components as steroids, biological matrices, and polyaromatic compounds (17)(18)(19)(20)(21)(22)(23). All these applications involved the installation of an additional heating source at the SFC sample outlet.…”

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confidence: 99%

Capillary supercritical fluid chromatography‐atmospheric pressure chemical ionization mass spectrometry of triacylglycerols in berry oils

Manninen

Laakso

1997

J Americ Oil Chem Soc

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A capillary supercritical fluid chromatograph (SFC), combined with a triple-quadrupole mass spectrometer (MS) via a liquid chromatography-atmospheric pressure chemical ionization (LC-APCI) interface, was utilized in the analysis of berry oil triacylglycerols. No modification of the commercially available interface was required. Vapor of different solvents, such as methanol, isopropanol, water, or ammonium hydroxide in methanol, was introduced in the sheath gas flow in the APCI source to achieve adequate ionization of triacylglycerols. The separation of triacylglycerols according to acyl carbon number and degree of unsaturation was accomplished on a 20 m × 50 µm i.d. SB-Cyanopropyl-25 column. The resolution of triacylglycerols in the reconstructed ion chromatogram and the sensitivity of the SFC-(APCI)MS system was comparable to or slightly better than that obtained with a flame ionization detector. No baseline drifting was observed during the SFC density programming. Triacylglycerols formed diagnostic [M + H] + and [M − RCOO] + ions with all tested reactant ion solvents except with ammonium hydroxide in methanol, which formed abundant [M + 18] + ions instead of [M + H] + ions. The abundance of the [M + H] + ion increased with increasing degree of unsaturation of a triacylglycerol, whereas the abundance of the [M − RCOO] + ion depended on the regiospecific distribution of the fatty acid moiety between the sn-1/3 positions and the sn-2 position and on the number of double bonds.

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“…For pSFC, the transposition is more complex because of the necessity to regulate pressure. API-MS interfaces are commonly modified to incorporate the restrictor at the end of the MS source capillary [9,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27]. However, in the case of APCI-MS, an unmodified LC interface has recently been introduced for pSFC with pure-CO 2 mobile phase [2].…”

mentioning

confidence: 99%

“…Moreover, separations are often different from and complementary to those obtained in LC [3][4][5][6][7][8]. pSFC mainly uses LC-like detectors, such as UV, evaporative light-scattering detection (ELSD) [3][4][5][6][7][8][9][10], or atmospheric-pressure ionisation mass-spectrometry (API-MS) [2,9,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] including ionspray or electrospray (IS-MS) and atmospheric-pressure chemical-ionisation mass spectrometry (APCI-MS). ELSD and API-MS are low-pressure detectors especially appropriate to non-UV-absorbing analytes.…”

mentioning

confidence: 99%

SFC with evaporative light-scattering detection and atmospheric-pressure chemical-ionisation mass spectrometry for methylated glucoses and cyclodextrins analysis

Herbreteau¹,

Salvador²,

Lafosse³

et al. 1999

Analusis

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Packed-column supercritical-fluid chromatography (pSFC) and related chromatography (e.g. subcritical-fluid or superheated-water chromatography) have a great deal to offer and are under-utilised techniques [1,2]. Column equilibration and analysis times are shorter than in liquid chromatography (LC). Moreover, separations are often different from and complementary to those obtained in LC [3][4][5][6][7][8]. pSFC mainly uses LC-like detectors, such as UV, evaporative light-scattering detection (ELSD) [3][4][5][6][7][8][9][10], or atmospheric-pressure ionisation mass-spectrometry (API-MS) [2,9,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] including ionspray or electrospray (IS-MS) and atmospheric-pressure chemical-ionisation mass spectrometry (APCI-MS). ELSD and API-MS are low-pressure detectors especially appropriate to non-UV-absorbing analytes. They both require nebulization and vaporization of the mobile phase. For this reason, ELSD was proven to be a good and inexpensive approach for method development in LC [28][29][30]. Transposition of separation methods from LC-ELSD to LC-API-MS was successful. For pSFC, the transposition is more complex because of the necessity to regulate pressure. API-MS interfaces are commonly modified to incorporate the restrictor at the end of the MS source capillary [9,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27]. However, in the case of APCI-MS, an unmodified LC interface has recently been introduced for pSFC with pure-CO 2 mobile phase [2].In the present work, with CO 2 -modifier mobile-phases, the ELSD interface design was studied with the intention of placing the restrictor at the column outlet inside the oven. Test solutes were methylated glucoses [7] and methylated-β-cyclodextrins (MβCD) [6,31]. MβCD are cyclic glucose oligomers methylated in various positions which are difficult to characterize. Once the interface design was optimized, pSFC-ELSD conditions were directly applied to pSFC-APCI-MS to provide structural information on complex MβCD mixtures. ExperimentalSFC analyses were conducted with an SF3 system (Gilson, Villiers-le-Bel, France) including CO 2 and modifier pumps, a 7125 injection valve with 20 µl sample loop (Rheodyne, Berkeley, CA, USA), and a Croco-Cil TM column oven (CILCluzeau, Sainte-Foy-la-Grande, France). The columns used were Zorbax Sil, 7 µm, 4.6 × 150 mm (Dupont, Wilmington, DE, USA) and Nucleosil NO 2 ,10 µm × 4.6 × 150 mm (Shandon, Cheshire, UK).The ELSD Sedex 55 (Sedere, Alfortville, France) was used with SFC or LC interface. First experiments were made with the commercial ELSD-SFC interface (Fig. 1a) equipped with a linear restrictor (fused silica, 5 cm length, 50 µm ID). For the study of the interface design, this restrictor was placed inside the oven at the column outlet, and a nebulization capillary (5 cm length; either fused silica, 50, 75 or 100 µm ID; or PEEK, 130 or 180 µm ID) was used as the SFC interface (Fig. 1b). A PEEK tubing (75 cm length, 130 µm ID) was used as the transfer line between t...

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Identification of the lipids and the ant attractant 1,2-dioleoylglycerol in the arils of Commiphora guillaumini Perr. (Burseraceae) by supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry

Cited by 14 publications

References 16 publications

Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids

Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids

Capillary supercritical fluid chromatography‐atmospheric pressure chemical ionization mass spectrometry of triacylglycerols in berry oils

SFC with evaporative light-scattering detection and atmospheric-pressure chemical-ionisation mass spectrometry for methylated glucoses and cyclodextrins analysis

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