Identification of the metabolites in normal and AA rat plasma, urine and feces after oral administration of Daphne genkwa flavonoids by LC-Q-TOF-MS spectrometry

Gan, Lu; Ji, Junfu; Wang, Ling; Li, Qiyan; Chunfeng, Zhang; Wang, Chong‐Zhi; Yuan, Chun‐Su

doi:10.1016/j.jpba.2019.112856

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…Flavonoid glycosides usually lose the glycosyl, the relative molecular weights (MW) of 146 Da (rhamnose), 162 Da (glucose) and 308 Da (rutinose or new neohesperidose) or the m / z 120 ion fragment, which are formed by cross‐ring excision of sugar. We detected other neutral losses, which included CH 3 (15 Da), CH 2 O (30 Da), CO (28 Da), CO 2 (44 Da) and H 2 O (18 Da), as well (Chen et al, 2019; Gan et al, 2020).…”

Section: Resultsmentioning

confidence: 84%

Rapid analysis of components in Qizhiweitong tablets and plasma after oral administration in rats by UPLC–Q‐TOF–MS/MS based on a self‐developed database

Bao

Wang

et al. 2022

Biomedical Chromatography

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Qizhiweitong is a famous traditional Chinese prescription medicine. It has been used to treat various stomach disorders, such as functional dyspepsia, chronic gastritis and intestinal stress syndrome, for a long time and gives favorable therapeutic effects in clinical settings. However, its chemical composition and possible bioactive components are not completely known. In the present study, we used ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS) and qualitatively analyzed the chemical composition of Qizhiweitong tablet extract and the absorbed prototype constituents along with corresponding metabolites in rat plasma following oral administration of Qizhiweitong tablet on the basis of our self-developed component database that was established accurately and rapidly. We detected a total of 119 compounds and 61 xenobiotics in the Qizhiweitong tablet, which included 32 prototypes and 28 metabolites. The results of the present study laid a solid foundation for quality marker screening and an integrative pharmacology-based study on the Qizhiweitong tablet. K E Y W O R D S absorption profiles in vivo, chemical profiles in vitro, Qizhiweitong tablet, UPLC-Q-TOF-MS/MS 1 | INTRODUCTION Qizhiweitong tablet (QZWTT) is a popular traditional Chinese medicine. It contains six herbal medicines, namely Bupleuri Radix (Chaihu), Cyperi Rhizoma (Xiangfu), Aurantii Fructus (Zhiqiao), Corydalis Rhizoma (Yanhusuo), Paeoniae Radix Alba (Baishao) and Glycyrrhizae Radix Et Rhizoma (Gancao). The prescription for QZWTT is obtained from the classic prescription Sinisan in the treatise on Febrile Diseases written by the medical sage Zhang Zhongjing. QZWTT is prepared by

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Section: Resultsmentioning

confidence: 84%

Rapid analysis of components in Qizhiweitong tablets and plasma after oral administration in rats by UPLC–Q‐TOF–MS/MS based on a self‐developed database

Bao

Wang

et al. 2022

Biomedical Chromatography

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“…In order to address the low fragmentation efficiency of in‐source CID, the high collision energy characteristic of MS ALL technique was applied to LC–Q‐TOF‐MS. LC–Q‐TOF‐MS is a hybrid MS technology consisted of a unit mass resolution quadrupole (Q1), a second quadrupole (Q2), and a TOF mass analyzer with high resolution [211–218]. In the acquisition procedure of MS ALL technique, all the precursor ions are allowed to go through Q1 freely and fragmented in Q2.…”

Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

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Polyethylene glycols are synthetic polymers composed of repeating oxyethylene subunits, which have been known for non-toxic, non-immunogenic, non-antigenic, good solubility in water and therefore approved for pharmaceutical applications. Recently, attachment or amalgamation of polyethylene glycols to therapeutic small molecules, peptides, proteins, or nanoparticles has become a mature technology for the sake of improving their pharmacokinetic and pharmacological profiles, also referred to as PEGylation. By comparison, there are only a few PEGylated pharmaceuticals have been registered for further clinical trials and even less was approved for marketing. High failure rate of PEGylated pharmaceuticals in pre-clinical and clinical trials could be majorly attributed to their unclear pharmacokinetic behaviors. Therefore, the in vivo fate of the PEGylated pharmaceuticals for the various routes of administration needs to be thoroughly investigated An accurate in vivo pharmacological study thereof highly depends on the precise detection of polyethylene glycols as well as their fragments in biological matrixes. The goal of this review is to highlight the analytical methods that were developed and applied to evaluate the polyethylene glycols in pharmaceutical ingredients and excipients, which bring us closer to bridging

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“…Therefore, determining the changes in the chemical composition of CHDG after processing and elucidating the cause of the changed curative effects are essential. Research into the medicinal properties of DG is mainly focused on chemical composition identification [ 20 , 21 ], quality control analysis [ 22 , 23 ], pharmacodynamic and toxicological evaluation [ 24 , 25 , 26 ], and pharmacokinetic studies [ 27 , 28 ]. In contrast, few studies have combined TCM processing with changes in the chemical composition of DG.…”

Section: Introductionmentioning

confidence: 99%

Identification of Daphne genkwa and Its Vinegar-Processed Products by Ultraperformance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry and Chemometrics

Zhang

Yao

et al. 2023

Molecules

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Crude herbs of Daphne genkwa (CHDG) are often used in traditional Chinese medicine to treat scabies baldness, carbuncles, and chilblain owing to their significant purgation and curative effects. The most common technique for processing DG involves the use of vinegar to reduce the toxicity of CHDG and enhance its clinical efficacy. Vinegar-processed DG (VPDG) is used as an internal medicine to treat chest and abdominal water accumulation, phlegm accumulation, asthma, and constipation, among other diseases. In this study, the changes in the chemical composition of CHDG after vinegar processing and the inner components of the changed curative effects were elucidated using optimized ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Untargeted metabolomics, based on multivariate statistical analyses, was also used to profile differences between CHDG and VPDG. Eight marker compounds were identified using orthogonal partial least-squares discrimination analysis, which indicated significant differences between CHDG and VPDG. The concentrations of apigenin-7-O-β-d-methylglucuronate and hydroxygenkwanin were considerably higher in VPDG than those in CHDG, whereas the amounts of caffeic acid, quercetin, tiliroside, naringenin, genkwanines O, and orthobenzoate 2 were significantly lower. The obtained results can indicate the transformation mechanisms of certain changed compounds. To the best of our knowledge, this study is the first to employ mass spectrometry to detect the marker components of CHDG and VPDG.

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Identification of the metabolites in normal and AA rat plasma, urine and feces after oral administration of Daphne genkwa flavonoids by LC-Q-TOF-MS spectrometry

Cited by 11 publications

References 19 publications

Rapid analysis of components in Qizhiweitong tablets and plasma after oral administration in rats by UPLC–Q‐TOF–MS/MS based on a self‐developed database

Rapid analysis of components in Qizhiweitong tablets and plasma after oral administration in rats by UPLC–Q‐TOF–MS/MS based on a self‐developed database

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Identification of Daphne genkwa and Its Vinegar-Processed Products by Ultraperformance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry and Chemometrics

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