Identification of the nasopharyngeal carriage of Neisseria meningitidis by 16S rRNA Gene sequencing in asymptomatic adolescents and young adults in Cartagena, Colombia (2019–2020).

Macias-Mendoza, Marlon; Montes-Robledo, Alfredo; Arteta-Acosta, Cindy; Baldiris-Avila, Rosa; Coronell-Rodríguez, Wilfrido

doi:10.1016/j.bjid.2022.102330

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Vaccinating adolescents can therefore also help to protect other age groups [ 7 , 8 ]. However, meningococcal nasopharyngeal carriage studies in Colombia have reported much lower carriage rates of 6.9% among 15–21-year-olds in Bogotá in 2012 [ 38 ] and 1.9% among 11–25-year-olds in Cartagena in 2019 [ 39 ]). In the current study, the highest burden of IMD during 2010–2021 was seen among infants aged < 1 year, with a mean incidence of approximately 1 per 100,000 and a mean mortality of around 0.3 per 100,000.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Invasive Meningococcal Disease in Colombia: A Retrospective Surveillance Database Analysis

Coronell-Rodriguez,

Caceres,

Cintra

et al. 2023

Infect Dis Ther

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Introduction Invasive meningococcal disease (IMD), caused by Neisseria meningitidis , is associated with high morbidity and mortality. The aim of the current study was to describe the historical and recent epidemiology of IMD in Colombia. Methods This retrospective surveillance database analysis examined all available data on IMD in Colombia. Data were extracted from publicly available disease event reports and laboratory surveillance reports or obtained directly from hospitals in Cartagena. Results During 2015–2021, the overall incidence of IMD was 0.04–0.18 per 100,000 based on laboratory surveillance reports. IMD incidence was highest among infants aged < 1 year (0.52–1.47 per 100,000), as was IMD mortality (0.00–0.65 per 100,000). Serogroup B was the dominant serogroup responsible for IMD in Colombia during 1988–2014, but, since 2015, serogroup C has been dominant in all age groups, followed by serogroups B and Y. During 2010–2021 combined, the majority of IMD cases were reported in Bogotá (31.9%) and Antioquia (21.7%). Of 42 IMD cases in the city of Cartagena, 54.8% occurred in people who lived in the poorest neighborhoods, and these patients had the highest IMD lethality (52.2%) and the shortest median hospitalization duration (3 days). Conclusion The overall incidence of IMD in Colombia was low but was highest among infants aged < 1 year. IMD cases tended to be concentrated in the more densely populated areas and in poorer neighborhoods. As the majority of IMD cases in Colombia since 2015 have been serogroup C, followed by B or Y, vaccination to protect against these serogroups could potentially be beneficial and help to achieve the World Health Organization’s and Pan American Health Organization’s roadmaps to defeat meningitis by 2030. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s40121-023-00886-y.

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Section: Discussionmentioning

confidence: 99%

Epidemiology of Invasive Meningococcal Disease in Colombia: A Retrospective Surveillance Database Analysis

Coronell-Rodriguez,

Caceres,

Cintra

et al. 2023

Infect Dis Ther

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Evaluation of antibody responses in healthcare workers before & after meningococcal vaccine and determination of meningococcal carriage rates

Baskol Elik,

Yıldırım,

Akyol Seyhan

et al. 2024

Vaccine

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Asymptomatic nasopharyngeal carriage of multidrug resistant bacteria among children at University of Gondar Hospital Northwest Ethiopia Revealing Hidden Health Risks

Birhanu,

Amare,

Tigabie

et al. 2024

Sci Rep

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Gram-negative bacteria in the nasopharynx can eventually invade bacteria-limited sites and cause serious illnesses such as meningitis, otitis media, and pneumonia. However, data related to the carriage of these bacteria in children attending outpatient departments in the study area are limited. To assess nasopharyngeal carriage, antibiotic susceptibility patterns, and associated factors of gramnegative bacteria among children attending the outpatient department at the University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia. A hospital-based cross-sectional study was conducted from September 1, 2023, to December 30, 2023. A total of 385 children aged 3 to 14 years were enrolled using a systematic random sampling technique. Sociodemographic and clinical data were collected using a semistructured questionnaire. A total of 385 nasopharyngeal samples were collected using a sterile specimen collection nasopharyngeal swab, transported using Amie's transport medium, and subsequently inoculated on chocolate agar, blood agar, modified thayer martin agar, and MacConkey agar plates. Bacterial species were identified by colony morphology, Gram staining, and biochemical tests such as oxidase tests, satellitism tests, and carbohydrate utilization tests. An antibiotic susceptibility test was performed using the Kirby-Bauer and modified Kirby-Bauer methods on Mueller-Hinton agar plates. The data were entered into Epi-Data version 4.6.0.6 and exported to SPSS version 25 for analysis. The adjusted odds ratio at a 95% confidence interval with a P value of < 0.05 in the binary logistic regression model was considered to indicate statistical significance. The overall nasopharyngeal carriage of gram-negative bacteria was 146 (37.9%) (95% CI: 33.2-42.9). Among these, nonfastidious gram-negative bacteria represented 45 (11.7%), followed by M. catarrhalis 41 (10.6%), N. meningitidis 34 (8.8%), and H. influenzae 26 (6.8%). The isolates exhibited high resistance to tetracycline (85; 75.9%), trimethoprim-sulfamethoxazole (105; 71.9%), ampicillin (76; 67.9%), and amoxicillin/clavulanic acid (60; 69.8%) but high susceptibility to meropenem (122; 83.6%), gentamicin (73; 84.9%), and minocycline (87; 72.5%). There were 99 total multidrug-resistant strains (67.8%, 95% CI: 59.7-75.0). Male sex (AOR = 1.785, 95% CI: 1.102-2.892, P = 0.019), smoking (AOR = 2.675, 95% CI: 1.149-6.230, P = 0.022), and large family size (≥ 5) (AOR = 1.857, 95% CI: 1.140-3.023, P = 0.013) were risk factors for nasopharyngeal colonization. Increased nasopharyngeal colonization of multidrug-resistant gram-negative isolates was observed in this study. Gentamicin, minocycline, and meropenem were the most effective antibiotics for the tested isolates. Bacterial colonization increased with increasing family size, smoking status, and male sex. Therefore, a definitive diagnosis in the outpatient pediatric department should be based on culture and susceptibility test results.

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Identification of the nasopharyngeal carriage of Neisseria meningitidis by 16S rRNA Gene sequencing in asymptomatic adolescents and young adults in Cartagena, Colombia (2019–2020).

Cited by 3 publications

References 45 publications

Epidemiology of Invasive Meningococcal Disease in Colombia: A Retrospective Surveillance Database Analysis

Epidemiology of Invasive Meningococcal Disease in Colombia: A Retrospective Surveillance Database Analysis

Evaluation of antibody responses in healthcare workers before & after meningococcal vaccine and determination of meningococcal carriage rates

Asymptomatic nasopharyngeal carriage of multidrug resistant bacteria among children at University of Gondar Hospital Northwest Ethiopia Revealing Hidden Health Risks

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