Identification of the novel HLA allele, <i>HLA‐B*35:03:23</i>, by next‐generation sequencing

Seshasubramanian, Vani; SathishKannan, Aruna Devi; Naganathan, Chandramouleeswaran; Manisekar, N. K.; Narayan, Saranya

doi:10.1111/tan.13459

Cited by 3 publications

(1 citation statement)

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“…Another sample showed a single‐base mismatch with B*35:03:01 in exon 1, which leads to a synonymous change in the amino acid in the protein derived from exon 1 (Gen bank Accession number MH192365). The name B*35:03:23 has been officially assigned for this novel allele by the WHO Nomenclature Committee in July 2018 and has been published . This follows the agreed policy that, subject to the conditions stated in the most recent Nomenclature Report, names will be assigned to new sequences as they are identified.…”

Section: Resultsmentioning

confidence: 99%

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

Seshasubramanian¹,

Venugopal²,

Kannan³

et al. 2019

HLA

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Cord blood has become an acceptable source of hematopoietic stem cells for transplantation. HLA plays a major role in hematopoietic stem cell transplantation (HSCT). Typing of cord blood samples for HLA alleles has been performed based on the serological and molecular methods. However, with the advent of nextgeneration sequencing technology, HLA typing becomes more accurate and unambiguous (upto intron level). Contamination of cord blood cells with erythropoietic cells poses a challenge in DNA extraction and downstream application. In the present study, DNA extracted from buffy coat of cord blood samples was typed for HLA-A, -B, -C, DRB1, and DQB1 alleles by Illumina miniseq and the sequences were aligned, phased, and mapped by MIA FORA software algorithms. Most frequent alleles found were HLA Afield resolution) HLA typing can be performed on cord blood samples. In order to preserve the precious sample for future use, minimal amount of cord blood samples (postprocessing) could be used for HLA typing purpose. K E Y W O R D S cord blood units, high-throughput typing, HLA typing, NGS

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Section: Resultsmentioning

confidence: 99%

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

Seshasubramanian¹,

Venugopal²,

Kannan³

et al. 2019

HLA

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Nomenclature for factors of the HLA system, update January, February and March 2019

Marsh¹

2019

Int J Immunogenetics

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Nomenclature for factors of the HLA system, update January, February and March 2019

Marsh¹

2019

HLA

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Identification of the novel HLA allele, HLA‐B35:03:23*, by next‐generation sequencing

Abstract: The novel HLA‐B allele, HLA‐B35:03:23, differs from B35:03:01 by a single‐nucleotide mismatch in exon 1.

Cited by 3 publications

References 2 publications

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

Nomenclature for factors of the HLA system, update January, February and March 2019

Nomenclature for factors of the HLA system, update January, February and March 2019

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Identification of the novel HLA allele, HLA‐B*35:03:23, by next‐generation sequencing

Abstract: The novel HLA‐B allele, HLA‐B*35:03:23, differs from B*35:03:01 by a single‐nucleotide mismatch in exon 1.

Cited by 3 publications

References 2 publications

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

Application of high‐throughput next‐generation sequencing for HLA typing of DNA extracted from postprocessing cord blood units

Nomenclature for factors of the HLA system, update January, February and March 2019

Nomenclature for factors of the HLA system, update January, February and March 2019

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Product

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Identification of the novel HLA allele, HLA‐B35:03:23*, by next‐generation sequencing

Abstract: The novel HLA‐B allele, HLA‐B35:03:23, differs from B35:03:01 by a single‐nucleotide mismatch in exon 1.