Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced by<i>Staphylococcus hominis</i>KQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)

Wilaipun, Pongtep; Zendo, Takeshi; Okuda, Ken Ichi; Nakayama, Jiro; Sonomoto, Kenji

doi:10.1271/bbb.80239

Cited by 40 publications

(22 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nukacin L217 is composed of 27 amino acid residues and showed high similarity (two different amino acids) with nukacin KQU-131 produced by Staph. hominis KQU-131 (Wilaipun et al 2008). These highly similar amino acid sequences suggest an important role for nukacin-type bacteriocins produced by different species of CoNS.…”

Section: Discussionmentioning

confidence: 85%

“…hominis KQU‐131 (Wilaipun et al . ). These highly similar amino acid sequences suggest an important role for nukacin‐type bacteriocins produced by different species of CoNS.…”

Section: Discussionmentioning

confidence: 97%

“…) and Staphylococcus hominis KQU‐131 (Wilaipun et al . ), respectively, belong to class I (Bastos et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…A generally accepted classification subdivides bacteriocins into four classes: class I or post-translationally modified lantibiotic bacteriocins; class II or nonmodified peptide bacteriocins; class III or large, heat-labile proteins; and class IV or cyclic peptides (Heng and Tagg 2006). According to this classification, most bacteriocins produced by CoNS, such as epidermin, hyicin 3682, nukacin ISK-1, nukacin 3299 and nukacin KQU-131, produced by Staphylococcus epidermidis T€ u 3298 (Allgaier et al 1985), Staphylococcus hyicus 3682 (Fagundes et al 2011), Staphylococcus warneri ISK-1 (Sashihara et al 2000), Staphylococcus simulans 3299 (Ceotto et al 2010) and Staphylococcus hominis KQU-131 (Wilaipun et al 2008), respectively, belong to class I (Bastos et al 2009).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

et al. 2014

View full text Add to dashboard Cite

Aims: To explore antibacterial activities of coagulase-negative staphylococci (CoNS) from teat apices of dairy cows towards mastitis-causing pathogens. Methods and Results: Of 254 CoNS, 38 displayed bacteriocin-like activity after a first screening. Seven of these strains displayed activity against at least one mastitis-related pathogen (Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus). Staphylococcus chromogenes L217 displayed the strongest inhibitory effect, being active against all tested mastitis-related pathogens and most tested CoNS. Based on cation exchange and reversed-phase chromatography, in addition to N-terminal Edman degradation and PCR, the antibacterial peptide was identified as a nukacin-type bacteriocin and named nukacin L217. Although staphylococcal bacteriocins are generally found in the cell-free supernatants of liquid cultures, Staph. chromogenes L217 only led to detectable activity when grown on agar medium. Conclusions: Bacteriocin-like activities are not uncommon among CoNS from teat apices and may inhibit mastitis-causing pathogens, as found for nukacin L217 production by Staph. chromogenes L217. Significance and Impact of the Study: Nukacin L217 is the first identified bacteriocin of the species Staph. chromogenes and displays unusual production kinetics, that is, requiring surface growth of its producer. The fact that nukacins are produced by different CoNS species suggests a role in the teat skin ecosystem.

show abstract

Section: Discussionmentioning

confidence: 85%

“…hominis KQU‐131 (Wilaipun et al . ). These highly similar amino acid sequences suggest an important role for nukacin‐type bacteriocins produced by different species of CoNS.…”

Section: Discussionmentioning

confidence: 97%

“…) and Staphylococcus hominis KQU‐131 (Wilaipun et al . ), respectively, belong to class I (Bastos et al . ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The immunity mechanism against nukacin ISK-1 mediated by NukFEG and NukH has been investigated before (2,(21)(22)(23)39). On the basis of our analysis, we suggested that NukFEG transports both nukacin ISK-1 on the membrane and nukacin ISK-1 captured by NukH (2,22).…”

mentioning

confidence: 74%

Functional Significance of the E Loop, a Novel Motif Conserved in the Lantibiotic Immunity ATP-Binding Cassette Transport Systems

Okuda¹,

Yanagihara²,

Sugayama³

et al. 2010

J Bacteriol

Self Cite

View full text Add to dashboard Cite

Lantibiotics are peptide-derived antibacterial substances produced by some Gram-positive bacteria and characterized by the presence of unusual amino acids, like lanthionines and dehydrated amino acids. Because lantibiotic producers may be attacked by self-produced lantibiotics, they express immunity proteins on the cytoplasmic membrane. An ATP-binding cassette (ABC) transport system mediated by the LanFEG protein complex is a major system in lantibiotic immunity. Multiple-sequence alignment analysis revealed that LanF proteins contain the E loop, a variant of the Q loop, which is a well-conserved motif in the nucleotide-binding domains (NBDs) of general ABC transporters. To elucidate E loop function, we introduced a mutation in the NukF protein, which is involved in the nukacin-ISK-1 immunity system. Amino acid replacement of glutamic acid in the E loop with glutamine (E85Q) resulted in slight decreases in the immunity level and transport activity. Additionally, the E85A mutation severely impaired the immunity level and transport activity. On the other hand, ATPase activities of purified E85Q and E85A mutants were almost similar to that of the wild type. These results suggested that the E loop found in ABC transporters involved in lantibiotic immunity plays a significant role in the function of these transporters, especially in the structural change of transmembrane domains.

show abstract

Fermented Foods in Health and Disease Prevention

Sahoo

Aradwad

Sanwal

et al. 2023

Microbes in the Food Industry

View full text Add to dashboard Cite

Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced byStaphylococcus hominisKQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)

Cited by 40 publications

References 18 publications

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

Functional Significance of the E Loop, a Novel Motif Conserved in the Lantibiotic Immunity ATP-Binding Cassette Transport Systems

Fermented Foods in Health and Disease Prevention

Contact Info

Product

Resources

About