This work presents the characterization of the pigments in the painting by Prayat Pongdam, one of the most essential artists in the history of Thai contemporary art. The painting that was studied is named “Cats and Birds (1957),” oil on board, from the collection of a private collector. XRF measurements were carried out utilizing a portable system and mapping scanning. XRF revealed substantial information about the pigments in the painting and the artist’s technique. Lead white (Pb) was presented in or near the ground layer. A mixture of barium (Ba) and titanium (Ti) was applied in almost all areas but less in the blue areas, presumably because these two pigments were used on top of the ground layer. Zinc (Zn) white was mixed with the cerulean blue (Co, Sn) for the blue regions. Chromium-based green (Cr) and red ochre (Fe) were in the palette in green and red areas, respectively. A layer of gold was applied to some areas, assuming the artist wanted to make it a highlight. Metallic pigments containing copper (Cu) were also revealed.