Identification of the Plasma Membrane Ca2+-ATPase and of Its Autoinhibitory Domain

Subcellular and Tissue Distribution, Partial Purification, and Sequencing of Calmodulin‐Stimulated Ca2+‐Transporting ATPases from Barley (Hordeum Vulgare L.) and Tobacco (Nicotiana Tabacum)

“…A 140-kDa protein that cross-reacts with antibodies against the erythrocyte Caz+ pump was reported to be present in maize coleoptiles (Briars et al, 1988), and the activity of a PM-located Ca2+ ATPase has been studied in maize leaves (Robinson et al, 1988), radish seeds (Rasi Caldogno et al, 1989, 1995, and Commelina communis leaves (Graf and Weiler, 1990). An intracellular CaM-stimulated Ca2+ transporter, presumably in the ER, was reported for maize roots (Brauer et al, 1990), carrot suspension cultures (Hsieh et al, 1991), cauliflower inflorescences (Askerlund and Evans, 1992) and barley aleurones (Gilroy and Jones, 1993).…”

Regulatory Role of the N Terminus of the Vacuolar Calcium-ATPase in Cauliflower

“…Although several studies have shown that the PM Ca 2ϩ -ATPase is CaM regulated (Rasi-Caldogno et al, 1995;Askerlund, 1997;Bonza et al, 1998;Olbe and Sommarin, 1998), nothing is known about either the location or sequence of the CaM binding region of this protein. We previously showed that anti-BCA1p cross-reacts with the PM Ca 2ϩ -ATPase (116 kD) in membrane fractions from cauliflower (Askerlund, 1996(Askerlund, , 1997 Four different membrane fractions from cauliflower inflorescence were used in western analysis: high-and lowdensity microsomal membrane fractions from a discontinuous Suc gradient centrifugation (Askerlund, 1996), a crude microsomal fraction, and a PM fraction obtained by two-phase partitioning.…”

Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H+-ATPase (III. Is There a Direct Interaction between the Fusicoccin Receptor and the Plasma Membrane H+-ATPase?)

“…Microsomes were extracted as previously described Rasi-Caldogno et al, 1993) and a PMenriched fraction was obtained by single-step aqueous twophase partitioning in Dextran T500 (Pharmacia)-PEG 3350 (Sigma) in the presence of 5 mM potassium-phosphate buffer (pH 7.8): 6.2% (w/w) polymers plus 3 mM KC1 for radish (Rasi-Caldogno et al, 1995) or 6% polymers and 0.5 mM KC1 for A. tkaliana :…”