1992
DOI: 10.1128/jb.174.12.3867-3873.1992
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Identification of the promoter region of the Escherichia coli major cold shock gene, cspA

Abstract: The major cold shock protein of Escheyichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10'C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstable at 37'C, so that CS7.4 production was hardly detected when the culture temperature was shifted from 15'C to 37°C. The transcription initiation site (+1) was identified. In vivo footprinting demonstrate… Show more

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Cited by 119 publications
(121 citation statements)
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“…3A). This contrasts those of E. coli CspA, whose induction by low temperature is more rapid and transient (21,42). The expression of WCSP1 transcript was not induced by environmental stress treatments such as salinity, drought, or ABA, all of which have been independently determined to induce the expression of various cold acclimation-related genes (Fig.…”
Section: Discussionmentioning
confidence: 82%
See 1 more Smart Citation
“…3A). This contrasts those of E. coli CspA, whose induction by low temperature is more rapid and transient (21,42). The expression of WCSP1 transcript was not induced by environmental stress treatments such as salinity, drought, or ABA, all of which have been independently determined to induce the expression of various cold acclimation-related genes (Fig.…”
Section: Discussionmentioning
confidence: 82%
“…CspA, the major cold shock protein of E. coli, accounts for more than 10% of total protein synthesis during the cold acclimation phase (20). The cspA gene has been cloned and sequenced, and primer extension studies have confirmed that cspA transcript levels also increase in response to cold shock (21). E. coli CspA binds to RNA to destabilize secondary structures; therefore, it was proposed that high levels of CspA could facilitate translation at low temperatures by eliminating secondary structures in mRNA (22).…”
mentioning
confidence: 96%
“…CspA is involved in transcriptional activation of certain cold-shock genes (Teana et a/., 1991;Jones et a/., 1992), and possibly functions as an RNA chaperone . The accumulation of CspA at low temperatures is due to the efficient translation of these mRNAs by ribosomes in cold-shocked cells (Brandi etal., 1996), by the increased stability of cspA transcripts at low temperatures (Brandi eta/., 1996;Goldenberg eta/., 1996), and by transcriptional up-regulation of the cspA gene (Goldenberg eta/., 1996;Tanabe et a/., 1992). Certain antibiotics, such as chloramphenicol, can induce the expression of some cold-shock proteins, and thus it has been proposed that the ribosome may be a sensor for the cold-shock response of E. coli (VanBogelen and Neidhardt, 1990;Jiang et a/., 1993).…”
Section: Discussionmentioning
confidence: 99%
“…Stabilization of the cspA mRNA by the three-base substitution mutation It has been speculated that the cold-shock induction of cspA occurs at the level of transcription (Goldstein et al, 1990;Tanabe et al, 1992). In the presence of chloramphenicol (30 g ml ¹1 ), not only the transcription of cspA is induced, but also the cspA mRNA becomes more stable, resulting in constitutive cspA expression (Jiang et al, 1993).…”
Section: Constitutive Expression Of Cspa At 37 њCmentioning
confidence: 99%