Identification of the release and effects of AHLs in anammox culture for bacteria communication

Tang, Xi; Liu, Sitong; Zhang, Zuotao; Zhuang, Guoqiang

doi:10.1016/j.cej.2015.03.045

Cited by 130 publications

(45 citation statements)

References 30 publications

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“…Most wastewater treatment systems depend on biofilm consortiums of heterotrophs and nitrifiers to effectively treat wastewater, and QS can play an important role in biofilm development (45). In addition, previous studies suggested a role for AHL QS in mixed populations carrying out anaerobic ammonium oxidation (anammox) (46,47). Although there has been recent interest in cycles of AHL production and destruction, via quorum quenching, in wastewater treatment systems, most of those studies focused on heterotrophs (45).…”

Section: Discussionmentioning

confidence: 99%

Acyl-Homoserine Lactone Production in Nitrifying Bacteria of the Genera Nitrosospira, Nitrobacter, and Nitrospira Identified via a Survey of Putative Quorum-Sensing Genes

Mellbye

Spieck

Bottomley

et al. 2017

Appl Environ Microbiol

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The genomes of many bacteria that participate in nitrogen cycling through the process of nitrification contain putative genes associated with acyl-homoserine lactone (AHL) quorum sensing (QS). AHL QS or bacterial cell-cell signaling is a method of bacterial communication and gene regulation and may be involved in nitrogen oxide fluxes or other important phenotypes in nitrifying bacteria. Here, we carried out a broad survey of AHL production in nitrifying bacteria in three steps. First, we analyzed the evolutionary history of AHL synthase and AHL receptor homologs in sequenced genomes and metagenomes of nitrifying bacteria to identify AHL synthase homologs in ammonia-oxidizing bacteria (AOB) of the genus and nitrite-oxidizing bacteria (NOB) of the genera, , and Next, we screened cultures of both AOB and NOB with uncharacterized AHL synthase genes and AHL synthase-negative nitrifiers by a bioassay. Our results suggest that an AHL synthase gene is required for, but does not guarantee, cell density-dependent AHL production under the conditions tested. Finally, we utilized mass spectrometry to identify the AHLs produced by the AOB and and the NOB and as -decanoyl-l-homoserine lactone (C-HSL), -3-hydroxy-tetradecanoyl-l-homoserine lactone (3-OH-C-HSL), a monounsaturated AHL (C-HSL), and -octanoyl-l-homoserine lactone (C-HSL), respectively. Our survey expands the list of AHL-producing nitrifiers to include a representative of lineage II and suggests that AHL production is widespread in nitrifying bacteria. Nitrification, the aerobic oxidation of ammonia to nitrate via nitrite by nitrifying microorganisms, plays an important role in environmental nitrogen cycling from agricultural fertilization to wastewater treatment. The genomes of many nitrifying bacteria contain genes associated with bacterial cell-cell signaling or quorum sensing (QS). QS is a method of bacterial communication and gene regulation that is well studied in bacterial pathogens, but less is known about QS in environmental systems. Our previous work suggested that QS might be involved in the regulation of nitrogen oxide gas production during nitrite metabolism. This study characterized putative QS signals produced by different genera and species of nitrifiers. Our work lays the foundation for future experiments investigating communication between nitrifying bacteria, the purpose of QS in these microorganisms, and the manipulation of QS during nitrification.

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Section: Discussionmentioning

confidence: 99%

Acyl-Homoserine Lactone Production in Nitrifying Bacteria of the Genera Nitrosospira, Nitrobacter, and Nitrospira Identified via a Survey of Putative Quorum-Sensing Genes

Mellbye

Spieck

Bottomley

et al. 2017

Appl Environ Microbiol

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“…To date, there are so many studies to enhance the activity of anammox biomass with additives, such as MnO 2 [26], ferrous ion [27], and acyl homoserine lactones 16 [28]. However, many kinds of these additives could result in secondary pollution.…”

Section: Preparation Of Biomass Extracts and Determination Of Enzymesmentioning

confidence: 98%

Fast start-up of the anammox process with addition of reduced graphene oxides

Yin

Qiao

Zhou

et al. 2016

Chemical Engineering Journal

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“…9 In other studies, C12-HSL, C8-HSL, and C6-HSL were detected in the supernatant of anammox cultures. 17 Thus far, however, the effects of exogenous signal molecules on heterotrophic nitrication have not been investigated in heterotrophic nitriers. In order to explore the role of AHLs in interspecic and intraspecic communication between microorganisms, we conduct a series of batch experiments to investigate Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Biofilm activity, ammonia removal and cell growth of the heterotrophic nitrifier,Acinetobactersp., facilitated by exogenousN-acyl-homoserine lactones

Wang

et al. 2018

RSC Adv.

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Identification of the release and effects of AHLs in anammox culture for bacteria communication

Cited by 130 publications

References 30 publications

Acyl-Homoserine Lactone Production in Nitrifying Bacteria of the Genera Nitrosospira, Nitrobacter, and Nitrospira Identified via a Survey of Putative Quorum-Sensing Genes

Acyl-Homoserine Lactone Production in Nitrifying Bacteria of the Genera Nitrosospira, Nitrobacter, and Nitrospira Identified via a Survey of Putative Quorum-Sensing Genes

Fast start-up of the anammox process with addition of reduced graphene oxides

Biofilm activity, ammonia removal and cell growth of the heterotrophic nitrifier,Acinetobactersp., facilitated by exogenousN-acyl-homoserine lactones

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