Identification of the sex of Oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequences.

Itoh, Yuichiro; Murata, Koichi; Hosoda, Takahisa; Mizuno, Shigeki

doi:10.1266/ggs.72.51

Cited by 33 publications

(24 citation statements)

References 8 publications

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“…USA 95 (1998)functions, the high level conservation of genomic sequences for both IREBP and ZOV3 in carinate and ratite birds seems to be reasonable. On the other hand, the relatively high level conservation of the EE0.6 sequence is somewhat surprising because it does not seem to retain a gene function in the present-day avian species (10,11). The most significant findings in this study with genomic probes for the emu and the ostrich homologues of EE0.6, IREBP, and ZOV3 are that the three loci were present on a particular pair of chromosomes in both the emu and the ostrich and that a definite sign of the morphological differentiation of W chromosome was noted in the ostrich with loss of a part of the chromosome arm containing the IREBP locus.…”

Section: Discussionmentioning

confidence: 99%

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The location of Z- and W-linked marker genes and sequence on the homomorphic sex chromosomes of the ostrich and the emu

Ogawa

Murata²,

Mizuno³

1998

Proc. Natl. Acad. Sci. U.S.A.

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106

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Perhaps the most striking fact about early Cenozoic avian history some 70 million years ago was the rapid radiation of large, f lightless, ground-living birds. It has been suggested that, for a time, there was active competition between these large terrestrial birds and the early mammals. Probably ref lecting the above noted early start of Ratitae of the infraclass Eoaves, the presumptive sex chromosomes of their present day survivors, such as the emu and the ostrich, largely remained homomorphic. The signs of genetic differentiation between their still-homomorphic Z and W chromosomes were tested by using two marker genes (Z-linked ZOV3 and the gene for the iron-responsive element-binding protein) and one marker sequence of a part of a presumptive pseudogene (W-linked EE0.6 of the chicken). Their homologues, maintaining 71-92% identities to the chicken counterparts, were found in both the emu (Dromaius novaehollandiae) and the ostrich (Struthio camelus). Their locations were visualized on chromosome preparations by f luorescence in situ hybridization. In the case of the emu, these three marker sequences were localized on both members of the fifth pair of a female, thus revealing no sign yet of genetic differentiation between the Z and the W. The finding was the same with regard to both members of the fourth pair of male ostriches. In the female ostrich, however, the sequence of the gene for the ironresponsive element-binding protein was missing from one of the pairs, thus revealing the differentiation by a small deletion of the W from the Z.

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Section: Discussionmentioning

confidence: 99%

“…nate species, such as the Oriental white stork (Ciconia boyciana) (10). With regard to this particular species, the Z-linked EE0.6-related sequence and the W-linked EE0.6 sequence shared 92% identity (11). In this paper, whether the conservation of a Z linkage group extends to ratite birds was tested with regard to ZOV3 and IREBP.…”

mentioning

confidence: 99%

The location of Z- and W-linked marker genes and sequence on the homomorphic sex chromosomes of the ostrich and the emu

Ogawa

Murata²,

Mizuno³

1998

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

106

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“…Whether this sequence contributes in any way to avian sex determination is not known. Molecular techniques have been used to identify sex in various studies of bird species ( Bradbury and Griffiths 1997;Ellegren et al 1996;Griffiths and Tiwari 1995;Itoh et al 1997;Komdeur 1996;Lessells and Mateman 1998;Millar et al 1996;Sheldon and Ellegren 1996). The techniques that have been used include searching for the female-specific variable number of tandem repeats (Rabenold et al 1991), identifying female-specific products using RAPD (Griffiths and Tiwari 1993), and using DNA probes that were selected from an avian clone library enriched for the W chromosome and hybridizing these clones to genomic DNA belonging to other avian species or groups.…”

Section: Discussionmentioning

confidence: 99%

Isolation of a Sex-Linked DNA Sequence in Cranes

Duan

Fuerst²

2001

Journal of Heredity

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A female-specific DNA fragment (CSL-W; crane sex-linked DNA on W chromosome) was cloned from female whooping cranes (Grus americana). From the nucleotide sequence of CSL-W, a set of polymerase chain reaction (PCR) primers was identified which amplify a 227-230 bp female-specific fragment from all existing crane species and some other noncrane species. A duplicated versions of the DNA segment, which is found to have a larger size (231-235 bp) than CSL-W in both sexes, was also identified, and was designated CSL-NW (crane sex-linked DNA on non-W chromosome). The nucleotide similarity between the sequences of CSL-W and CSL-NW from whooping cranes was 86.3%. The CSL primers do not amplify any sequence from mammalian DNA, limiting the potential for contamination from human sources. Using the CSL primers in combination with a quick DNA extraction method allows the noninvasive identification of crane gender in less than 10 h. A test of the methodology was carried out on fully developed body feathers from 18 captive cranes and resulted in 100% successful identification.

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“…The sequences of sexing primer for amplifying the W chromosome-specific DNA (ϳ190 bps) were 5Ј-ACAGTTTGTCTGTCTCCGGGGAA-3Ј (AWS03) and 5Ј-AGCTGGAYTTCAGWSCATCTT-CT-3Ј (USP3), and those of internal control primer for amplifying the Z/W chromosome-common DNA (ϳ250 bps) were 5Ј-CTCTGTCTGGAAGGACTT-3Ј (INT-R) and 5Ј-ATAGAAACAATGTGGGAC-3Ј (INT-F). Detailed information about these primers and related sequences was given elsewhere (Itoh et al 1997(Itoh et al , 2001Ogawa et al 1997).…”

Section: ) Pcr For Gender Determinationmentioning

confidence: 99%

External features and molecular sexing of the anomalous Pintail, Anas acuta, found at Hyo-ko Waterfowl Park, Niigata Prefecture, Japan

et al. 2002

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Identification of the sex of Oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequences.

Cited by 33 publications

References 8 publications

The location of Z- and W-linked marker genes and sequence on the homomorphic sex chromosomes of the ostrich and the emu

The location of Z- and W-linked marker genes and sequence on the homomorphic sex chromosomes of the ostrich and the emu

Isolation of a Sex-Linked DNA Sequence in Cranes

External features and molecular sexing of the anomalous Pintail, Anas acuta, found at Hyo-ko Waterfowl Park, Niigata Prefecture, Japan

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