Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens

Stachel, Scott E.; Messens, Eric; Montagu, Marc Van; Zambryski, Patricia

doi:10.1038/318624a0

Cited by 927 publications

(511 citation statements)

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“…Similar results have been reported by Levee et al [9]. Coniferyl alcohol is a phenolic compound that is released by the wounded plant cells and is a virulence inducer similar to acetosyringone [19] and syringaldehyde. It plays an important role in the natural infection of plants by Agrobacterium tumefaciens because it activates the virulence genes of the Ti pasmid that initiates the transfer of the T-DNA region into a plant.…”

Section: Discussionsupporting

confidence: 89%

Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)

Tang

2001

Cell Res

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This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for b-glucuronidase (GUS) and neomycin phosphotransferase (NPTII). Factors influencing transgene expression including seed sources of loblolly pine, concentration of bacteria, and the wounding procedures of target explants were investigated. The expression of foreign gene was confirmed by the ability of mature zygotic embryos to produce calli in the presence of kanamycin, by histochemical assays of GUS activity, by PCR analysis, and by Southern blot. The successful expression of the GUS gene in different families of loblolly pine suggests that this transformation system is probably useful for the production of the genetically modified conifers.

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Section: Discussionsupporting

confidence: 89%

Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)

Tang

2001

Cell Res

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“…Acetosyringone (AS) is a phenolic compound released by wounded cells of some dicotyledonous plant [8]. It plays an important role in the natural infection of plants by A. rhizogenes as it activates the virulence genes of the Ri plasmid which initiates transfer of the T-DNA region to the plant DNA.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

Wang

Luo

et al. 2001

Cell Res

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The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Plant regeneration was achieved from sterile calluses derived from hairy roots, which occurred at or near the infection sites. The hairy root regenerants were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T-DNA had been intergrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.

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“…It has been known for nearly a century that root exudates-the chemicals released from root systems into the soil-have profound effects on microorganisms in the rhizosphere (for review, see Curl and Truelove, 1986;Rovira, 1991). In recent years, specific sugars and phenolic compounds from plant exudates have been shown to induce microbial genes required for pathogenesis and symbiosis (Rossen et al, 1985;Stachel et al, 1985;Shearman et al, 1986;Peters and Long, 1988), and the cellular sources of such chemicals are now being examined (Maxwell and Phillips, 1990;Graham, 1991;Oommen et al, 1994;McKhann et al, 1997).…”

mentioning

confidence: 99%

“…tumejaciens viu regulon that is regulated in response to sugars and simple phenolic compounds (Stachel et al, 1985;Cangelosi et al, 1990). The common nod genes, which encode enzymes involved in the biosynthesis of signal molecules required for nodulation, are induced in response to specific flavonoid molecules from plant roots (Peters and Long, 1988;Lerouge et al, 1990;van Brussel et al, 1990).…”

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confidence: 99%

Induction of Microbial Genes for Pathogenesis and Symbiosis by Chemicals from Root Border Cells

1997

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Reporter strains of soil-borne bacteria were used to test the hypothesis that chemicals released by root border cells can influente the expression of bacterial genes required for the establishment of plant-microbe associations. Promoters from genes known to be activated by plant factors included vir€, required for Agrobacferium fumefaciens pathogenesis, and common nod genes from Rhizobium leguminosarum bv viciae and Rhizobium melilofi, required for nodulation of pea (Pisum sativum) and alfalfa (Medicago safivum), respectively. Also included was phzB, an autoinducible gene encoding the biosynthesis of antibiotics by Pseudomonas aureofaciens. The vir€ and nod genes were activated to different degrees, depending on the source of border cells, whereas phzB activity remained unaffected. The homologous interaction between R. leguminosarum bv viciae and its host, pea, was examined in

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Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens

Cited by 927 publications

References 20 publications

Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)

Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)

Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

Induction of Microbial Genes for Pathogenesis and Symbiosis by Chemicals from Root Border Cells

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