Identification of the syntrophic partners in a coculture coupling anaerobic methanol oxidation to Fe(III) reduction

Daniel, Rolf; Warnecke, Falk; Potekhina, Joanna S; Gottschalk, Gerhard

doi:10.1111/j.1574-6968.1999.tb08796.x

Cited by 20 publications

(9 citation statements)

References 27 publications

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“…Other authors proposed this mechanism as well (e.g. Daniel et al, 1999;Murase and Kimura, 1994b), but, besides circumstantial evidence from rice paddies (Miura et al, 1992), the only supporting data is from marine sediments (Beal et al, 2009 where Fe reduction accounted for 27-72% of anaerobic C mineralization in fens receiving exogenous Fe and 7% with only internally cycled Fe. In a further study in the same peatlands , Fe reduction was a significant process that inhibited methanogenesis.…”

Section: Biogeochemistry and Electron Acceptorsmentioning

confidence: 96%

“…4; Reaction 2), but the process was not observed in past studies of known Fe(III)-reducers or in AOM studies in peat soils (Smemo and Yavitt, 2007). A study by Daniel et al (1999) found that Fe(III) reduction can be coupled to methanol oxidation by a syntrophic relationship between Shewanella putrefacians and Clostridium sphenoides. They proposed a potentially beneficial reaction ( G • =-782 kJ reaction −1 ) and claimed that this reaction would be slow due to the nature of the syntrophic relationship, but they did not account for the fact that Fe(III) is not available at pH 7.0 in natural waters.…”

Section: Microbiologymentioning

confidence: 96%

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Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2011

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Abstract.Despite a large body of literature on microbial anaerobic oxidation of methane (AOM) in marine sediments and saline waters and its importance to the global methane (CH 4 ) cycle, until recently little work has addressed the potential occurrence and importance of AOM in non-marine systems. This is particularly true for peatlands, which represent both a massive sink for atmospheric CO 2 and a significant source of atmospheric CH 4 . Our knowledge of this process in peatlands is inherently limited by the methods used to study CH 4 dynamics in soil and sediment and the assumption that there are no anaerobic sinks for CH 4 in these systems. Studies suggest that AOM is CH 4 -limited and difficult to detect in potential CH 4 production assays against a background of CH 4 production. In situ rates also might be elusive due to background rates of aerobic CH 4 oxidation and the difficulty in separating net and gross process rates. Conclusive evidence for the electron acceptor in this process has not been presented. Nitrate and sulfate are both plausible and favorable electron acceptors, as seen in other systems, but there exist theoretical issues related to the availability of these ions in peatlands and only circumstantial evidence suggests that these pathways are important. Iron cycling is important in many wetland systems, but recent evidence does not support the notion of CH 4 oxidation via dissimilatory Fe(III) reduction or a CH 4 oxidizing archaea in consortium with an Fe(III) reducer. Calculations based on published rates demonstrate that AOM might be a significant and underappreciated constraint on the global CH 4 cycle, although much about the process is unknown, in vitro rates may not relate well to in situ rates, and projections based on those rates are fraught with uncertainty. We suggest electron transfer mechanisms, C flow and pathways, and quantifying in situ peatland AOM rates as the highest priority topics for future research.

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Section: Biogeochemistry and Electron Acceptorsmentioning

confidence: 96%

Section: Microbiologymentioning

confidence: 96%

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2011

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“…In the present experiment, iron concentrations ranged between 63 and 83 µg g −1 in the different treatments of the SI soil and between 120 and 140 µg g −1 in the CO soil. The theoretical amount of Fe(III) necessary for the oxidation of 500 µl methane is ca 73 µg g −1 soil (for reaction equation, see Daniel et al, 1999). Thus, enough Fe(III) has been in SI soil to serve as terminal electron acceptor for anaerobic oxidation of methane.…”

Section: Discussionmentioning

confidence: 99%

“…It was also proposed that Fe(III) could serve as electron acceptor for AOM in freshwater wetlands (Murase and Kimura, 1994;Daniel et al, 1999). The reaction is energetically favourable (Smemo and Yavitt, 2011).…”

Section: Discussionmentioning

confidence: 99%

Anaerobic oxidation of methane in grassland soils used for cattle husbandry

Bannert

Bogen²,

Esperschütz³

et al. 2012

Biogeosciences

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Abstract. While the importance of anaerobic methane oxidation has been reported for marine ecosystems, the role of this process in soils is still questionable. Grasslands used as pastures for cattle overwintering show an increase in anaerobic soil micro-sites caused by animal treading and excrement deposition. Therefore, anaerobic potential methane oxidation activity of severely impacted soil from a cattle winter pasture was investigated in an incubation experiment under anaerobic conditions using 13 C-labelled methane. We were able to detect a high microbial activity utilizing CH 4 as nutrient source shown by the respiration of 13 CO 2 . Measurements of possible terminal electron acceptors for anaerobic oxidation of methane were carried out. Soil sulfate concentrations were too low to explain the oxidation of the amount of methane added, but enough nitrate and iron(III) were detected. However, only nitrate was consumed during the experiment. 13 C-PLFA analyses clearly showed the utilization of CH 4 as nutrient source mainly by organisms harbouring 16 : 1ω7 PLFAs. These lipids were also found as most 13 Cenriched fatty acids by Raghoebarsing et al. (2006) after addition of 13 CH 4 to an enrichment culture coupling denitrification of nitrate to anaerobic oxidation of methane. This might be an indication for anaerobic oxidation of methane by relatives of "Candidatus Methylomirabilis oxyfera" in the investigated grassland soil under the conditions of the incubation experiment.

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“…Methanogens degrade methanol to methane and carbon dioxide (Schnellen, 1947 ;Sowers & Ferry, 1983 ;Ollivier et al, 1984 ;Ni & Boone, 1991). Methanol can also be degraded by syntrophic cultures of anaerobic micro-organisms (Heijthuijsen & Hansen, 1986 ;Cord-Ruwisch & Ollivier, 1986 ;Davidova & Stams, 1996 ;Daniel et al, 1999). However, none of these methanol-degrading micro-organisms is restricted to the use of methanol as a substrate.…”

Section: Of Co #mentioning

confidence: 99%

Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.

Balk¹,

Weijma²,

Stams³

2002

International Journal of Systematic and Evolutionary Microbiology

103

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Identification of the syntrophic partners in a coculture coupling anaerobic methanol oxidation to Fe(III) reduction

Cited by 20 publications

References 27 publications

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

Anaerobic oxidation of methane in grassland soils used for cattle husbandry

Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.

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