Identification of the TBX5 transactivating domain and the nuclear localization signal

Zaragoza, Michael V.; Lewis, Lisa E.; Sun, Guoqiang; Wang, Eric; Li, Ling; Said-Salman, Ilham; Feucht, Laura; Huang, Taosheng

doi:10.1016/j.gene.2004.01.017

Cited by 47 publications

(27 citation statements)

References 27 publications

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“…The nuclear availability of TBX5 is a measure of its transcriptional activity. The transactivation domain of the TBX5 protein is at the C-terminal part of the protein (amino acids 339-379) (Zaragoza et al, 2004). LMP4 (a PDZ-LIM protein) interacts with the transactivation domain and the TBX5-LMP4 complex mediates the dynamic shuttling of TBX5 between the nucleus and the cytoplasm (Kulisz and Simon, 2008).…”

Section: Literature Reviewmentioning

confidence: 99%

Molecular basis of the clinical features of Holt–Oram syndrome resulting from missense and extended protein mutations of the TBX5 gene as well as TBX5 intragenic duplications

Al‐Qattan

Al-Shaar

2015

Gene

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Section: Literature Reviewmentioning

confidence: 99%

Molecular basis of the clinical features of Holt–Oram syndrome resulting from missense and extended protein mutations of the TBX5 gene as well as TBX5 intragenic duplications

Al‐Qattan

Al-Shaar

2015

Gene

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“…10,16 In addition, Crm1-dependent nuclear export of TBX5 is thought to be mediated through recognition of a putative nuclear export signal (NES, Fig. 2b) located in the T-box domain (L 152 VSFQKLKL 160 ).…”

Section: Tbx5 3 10 -Helix C Is An Important Dna Recognition Elementmentioning

confidence: 99%

“…The C-terminal region following the T-box domain harbors a transactivation domain including a nuclear localization signal. 10 TBX5 is known to interact with promoter regions of several genes and among them the cardiac-specific natriuretic peptide precursor type A (ANF or Nppa) and the connexin (cx40) genes are best described. 7,11,12 It has been shown that TBX5 activates its target genes synergistically with the homeodomain transcription factor Nkx2-5.…”

Section: Introductionmentioning

confidence: 99%

Structural Basis of TBX5–DNA Recognition: The T-Box Domain in Its DNA-Bound and -Unbound Form

Stirnimann

Ptchelkine

Grimm

et al. 2010

Journal of Molecular Biology

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“…The four amino acids unique to moa Tbx5 at the NH 2 terminus are shown by white vertical lines. The position of Nuclear Localisation Signals 1 and 2 (NLS1, NLS2) and the Nuclear Export Signal (NES) are shown [52,53]. B .…”

Section: Resultsmentioning

confidence: 99%

Reconstruction and in vivo analysis of the extinct tbx5 gene from ancient wingless moa (Aves: Dinornithiformes)

et al. 2014

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BackgroundThe forelimb-specific gene tbx5 is highly conserved and essential for the development of forelimbs in zebrafish, mice, and humans. Amongst birds, a single order, Dinornithiformes, comprising the extinct wingless moa of New Zealand, are unique in having no skeletal evidence of forelimb-like structures.ResultsTo determine the sequence of tbx5 in moa, we used a range of PCR-based techniques on ancient DNA to retrieve all nine tbx5 exons and splice sites from the giant moa, Dinornis. Moa Tbx5 is identical to chicken Tbx5 in being able to activate the downstream promotors of fgf10 and ANF. In addition we show that missexpression of moa tbx5 in the hindlimb of chicken embryos results in the formation of forelimb features, suggesting that Tbx5 was fully functional in wingless moa. An alternatively spliced exon 1 for tbx5 that is expressed specifically in the forelimb region was shown to be almost identical between moa and ostrich, suggesting that, as well as being fully functional, tbx5 is likely to have been expressed normally in moa since divergence from their flighted ancestors, approximately 60 mya.ConclusionsThe results suggests that, as in mice, moa tbx5 is necessary for the induction of forelimbs, but is not sufficient for their outgrowth. Moa Tbx5 may have played an important role in the development of moa’s remnant forelimb girdle, and may be required for the formation of this structure. Our results further show that genetic changes affecting genes other than tbx5 must be responsible for the complete loss of forelimbs in moa.

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Identification of the TBX5 transactivating domain and the nuclear localization signal

Cited by 47 publications

References 27 publications

Molecular basis of the clinical features of Holt–Oram syndrome resulting from missense and extended protein mutations of the TBX5 gene as well as TBX5 intragenic duplications

Molecular basis of the clinical features of Holt–Oram syndrome resulting from missense and extended protein mutations of the TBX5 gene as well as TBX5 intragenic duplications

Structural Basis of TBX5–DNA Recognition: The T-Box Domain in Its DNA-Bound and -Unbound Form

Reconstruction and in vivo analysis of the extinct tbx5 gene from ancient wingless moa (Aves: Dinornithiformes)

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