Identification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica

Ivamoto, Suzana Tiemi; Domingues, Douglas Silva; Vieira, Luiz Gonzaga Esteves; Pereira, Luiz Filipe Protásio

doi:10.4238/2015.march.27.25

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…We identified one SNP associated with CAF (S11_29778697) that was co-localized with the gene Cc11_g12750, which encodes a cytochrome P450 704 ( CYP704 ). Several P450 genes are involved in secondary metabolite biosynthesis, including terpenoids 43 , 44 . CYP704 in rice was also shown to provide lipid monomers for the synthesis of anther cutin 45 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L

Sant’Ana

Pereira

Pot

et al. 2018

Sci Rep

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Lipids, including the diterpenes cafestol and kahweol, are key compounds that contribute to the quality of coffee beverages. We determined total lipid content and cafestol and kahweol concentrations in green beans and genotyped 107 Coffea arabica accessions, including wild genotypes from the historical FAO collection from Ethiopia. A genome-wide association study was performed to identify genomic regions associated with lipid, cafestol and kahweol contents and cafestol/kahweol ratio. Using the diploid Coffea canephora genome as a reference, we identified 6,696 SNPs. Population structure analyses suggested the presence of two to three groups (K = 2 and K = 3) corresponding to the east and west sides of the Great Rift Valley and an additional group formed by wild accessions collected in western forests. We identified 5 SNPs associated with lipid content, 4 with cafestol, 3 with kahweol and 9 with cafestol/kahweol ratio. Most of these SNPs are located inside or near candidate genes related to metabolic pathways of these chemical compounds in coffee beans. In addition, three trait-associated SNPs showed evidence of directional selection among cultivated and wild coffee accessions. Our results also confirm a great allelic richness in wild accessions from Ethiopia, especially in accessions originating from forests in the west side of the Great Rift Valley.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L

Sant’Ana

Pereira

Pot

et al. 2018

Sci Rep

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“…Since CAF and KAH are probable derivatives of ent-kaurene, and there are several reports attributing to P450s the stereospecific hydroxylation and oxidation of hydroxyl groups that leads to the synthesis of several diterpenes (Zerbe et al, 2013;Pateraki et al, 2015), we hypothesized that the transcriptional evaluation of selected P450 candidate genes could give us insights on the final steps from ent-kaurene to CAF and KAH biosynthesis. Therefore, to identify the best P450s candidate genes possibly related to the final steps of CAF and KAH formation we combined our biochemical data with the P450s transcriptional profile obtained from a previous in silico analysis (Ivamoto et al, 2015). Based on CAF and KAH concentrations, we selected five contrasting samples for diterpenes accumulation (leaves, flowers and perisperm at 90, 120 and 150 DAF) and five P450s candidate genes for the transcriptional analysis by RT-qPCR.…”

Section: Discussionmentioning

confidence: 99%

“…Seven P450 genes (Table 1) were selected from a previous study (Ivamoto et al, 2015) based in the function of putative orthologs and in silico expression patterns. The P450 candidate gene sequences were selected from the Brazilian Coffee Expressed Sequenced Project (CafEST) (http://www.lge.ibi.unicamp.br/cafe).…”

Section: Selection Of P450 Candidate Genesmentioning

confidence: 99%

“…Seven P450 candidate genes described as related to secondary metabolite biosynthesis (Ivamoto et al, 2015, Table 1) were selected for transcriptional analysis using RT-qPCR in the leaves, flowers and perisperm at 90, 120 and 150 DAF. These tissues were chosen for further analysis based on displaying the most contrasting CAF and KAH contents.…”

Section: Transcriptional Patterns Of P450 Candidate Genesmentioning

confidence: 99%

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Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development

Ivamoto

Sakuray

Ferreira

et al. 2017

Plant Physiology and Biochemistry

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Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30-240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways.

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Validation of reference genes for qPCR analysis of Coffea arabica L. somatic embryogenesis-related tissues

Freitas

Barreto

Fernandes-Brum

et al. 2016

Plant Cell Tiss Organ Cult

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Identification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica

Cited by 4 publications

References 40 publications

Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L

Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L

Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development

Validation of reference genes for qPCR analysis of Coffea arabica L. somatic embryogenesis-related tissues

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