Identification of the Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase homologue

Stebeck, Caroline E.

doi:10.1016/s0378-1097(97)00346-7

Cited by 13 publications

(20 citation statements)

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“…In the syphilis spirochaete, Treponema pallidum , one glpQ -like gene is present; however, the encoded protein is not active as a glycerophosphodiesterase. Again, the inactivity is most likely caused by the lack of conservation of functionally important amino acids [37], [38]. The presence of inactive GlpQ-like proteins in several pathogens, including a spirochaete and M. genitalium , the bacterium with the smallest genome, suggests that these proteins have other functions that have yet to be identified.…”

Section: Discussionmentioning

confidence: 99%

A Trigger Enzyme in Mycoplasma pneumoniae: Impact of the Glycerophosphodiesterase GlpQ on Virulence and Gene Expression

et al. 2011

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Mycoplasma pneumoniae is a causative agent of atypical pneumonia. The formation of hydrogen peroxide, a product of glycerol metabolism, is essential for host cell cytotoxicity. Phosphatidylcholine is the major carbon source available on lung epithelia, and its utilization requires the cleavage of deacylated phospholipids to glycerol-3-phosphate and choline. M. pneumoniae possesses two potential glycerophosphodiesterases, MPN420 (GlpQ) and MPN566. In this work, the function of these proteins was analyzed by biochemical, genetic, and physiological studies. The results indicate that only GlpQ is an active glycerophosphodiesterase. MPN566 has no enzymatic activity as glycerophosphodiesterase and the inactivation of the gene did not result in any detectable phenotype. Inactivation of the glpQ gene resulted in reduced growth in medium with glucose as the carbon source, in loss of hydrogen peroxide production when phosphatidylcholine was present, and in a complete loss of cytotoxicity towards HeLa cells. All these phenotypes were reverted upon complementation of the mutant. Moreover, the glpQ mutant strain exhibited a reduced gliding velocity. A comparison of the proteomes of the wild type strain and the glpQ mutant revealed that this enzyme is also implicated in the control of gene expression. Several proteins were present in higher or lower amounts in the mutant. This apparent regulation by GlpQ is exerted at the level of transcription as determined by mRNA slot blot analyses. All genes subject to GlpQ-dependent control have a conserved potential cis-acting element upstream of the coding region. This element overlaps the promoter in the case of the genes that are repressed in a GlpQ-dependent manner and it is located upstream of the promoter for GlpQ-activated genes. We may suggest that GlpQ acts as a trigger enzyme that measures the availability of its product glycerol-3-phosphate and uses this information to differentially control gene expression.

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Section: Discussionmentioning

confidence: 99%

A Trigger Enzyme in Mycoplasma pneumoniae: Impact of the Glycerophosphodiesterase GlpQ on Virulence and Gene Expression

et al. 2011

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“…Tp92 and Gpd are proteins that have homologues in a number of gram-negative bacteria (3,4,26). This may explain the reactivity of sera from some individuals with nonsyphilis spirochetal infections to these molecules.…”

Section: Vol 41 2003 Serodiagnosis Of Syphilis 3671mentioning

confidence: 99%

“…Recombinant proteins selected for screening included Gpd (Tp0257) (3,26) and Tp92 (Tp0326) (4), which were previously identified from a serologic screen as being reactive with syphilis patient sera, and proteins predicted by computer analyses of the genome to be putative outer membrane proteins. Since other spirochetal diseases such as Lyme disease, relapsing fever, and leptospirosis would be expected to have antigens most similar to T. pallidum, we used sera col-Preparation of recombinant proteins.…”

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confidence: 99%

Serodiagnosis of Syphilis: Antibodies to Recombinant Tp0453, Tp92, and Gpd Proteins Are Sensitive and Specific Indicators of Infection byTreponema pallidum

et al. 2003

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Syphilis serodiagnosis relies on a combination of nonspecific screening tests (antilipoidal antibodies) andTreponema pallidum-specific tests (anti-T. pallidum antibodies). We studied a group of six recombinant T. pallidum antigens for their sensitivities and specificities with sera from individuals with syphilis (n ‫؍‬ 43), relapsing fever (n ‫؍‬ 8), Lyme disease (n ‫؍‬ 8), and leptospirosis (n ‫؍‬ 9) and from uninfected individuals (n ‫؍‬ 15). Three recombinant proteins, Tp0155, Tp0483, and Tp0751, demonstrated sensitivity values that ranged from 28 to 42%. In contrast, three other recombinant proteins exhibited the following sensitivity and specificity values: Tp0453, 100% sensitivity and 100% specificity; Tp92 (Tp0326), 98% sensitivity and 97% specificity; and Gpd (Tp0257), 91% sensitivity and 93% specificity. Tp0453, Tp92, and Gpd also were recognized by sera from individuals with early primary syphilis that were nonreactive with the antilipoidal Venereal Disease Research Laboratory test. The reactivities of syphilis patient sera with Tp0453, Tp92, and Gpd were proportional to the titers of these sera with the treponemal test MHA-TP (microhemagglutination assay for T. pallidum). Thus, the recombinant T. pallidum antigens Tp0453, Tp92, and Gpd show promise as diagnostic antigens in the enzymelinked immunosorbent assay-based assay.

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“…pallidum Nichols strain genome (7), some of which code for candidate surface-exposed proteins (3,7,12,13). The tpr genes of T. pallidum are of interest for a variety of reasons.…”

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confidence: 99%

Multiple Alleles ofTreponema pallidumRepeat Gene D inTreponema pallidumIsolates

et al. 2000

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Identification of the Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase homologue

Cited by 13 publications

References 0 publications

A Trigger Enzyme in Mycoplasma pneumoniae: Impact of the Glycerophosphodiesterase GlpQ on Virulence and Gene Expression

A Trigger Enzyme in Mycoplasma pneumoniae: Impact of the Glycerophosphodiesterase GlpQ on Virulence and Gene Expression

Serodiagnosis of Syphilis: Antibodies to Recombinant Tp0453, Tp92, and Gpd Proteins Are Sensitive and Specific Indicators of Infection byTreponema pallidum

Multiple Alleles ofTreponema pallidumRepeat Gene D inTreponema pallidumIsolates

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