Identification of the Uncultured Bacillus of Whipple’s Disease

Relman, David A.; Schmidt, Thomas M.; MacDermott, Richard P.; Falkow, Stanley

doi:10.1056/nejm199207303270501

Cited by 1,095 publications

(584 citation statements)

References 36 publications

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“…En l'absence d'identité significative avec les espèces bacté-riennes déjà recensées, une nouvelle espèce bactérienne peut être décrite et positionnée dans l'arbre phylogénétique construit à partir des séquences d'ADNr 16S connues. La puissance de cette approche a été illustrée par l'identification de Tropheryma whippelii, espèce bactérienne associée à la maladie de Whipple [14], et alors non cultivable, ou de Bartonella henselae, associée à l'angiomatose bacillaire [15]. L'utilisation de cette technique à partir des échantillons intestinaux congelés de cette patiente a conduit à la mise en évi-dence de séquences appartenant à l'espèce Campylobacter jejuni [13].…”

Section: La Cible Thérapeutique De L'ezetimibe : Npc1-l1unclassified

Maladie immunoproliférative de l'intestin grêle associée à Campylobacter jejuni

Lecuit

Lortholary

2005

Médecine et Maladies Infectieuses

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Section: La Cible Thérapeutique De L'ezetimibe : Npc1-l1unclassified

Maladie immunoproliférative de l'intestin grêle associée à Campylobacter jejuni

Lecuit

Lortholary

2005

Médecine et Maladies Infectieuses

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“…Wilson (642). Recently, Relman and colleagues reported a technique in which oligonucleotide primers specific for the Whipple's disease bacillus were used in a PCR to amplify sequences of bacterial 16S rRNA directly from infected tissues (469). Following a comparative 16S rRNA sequence analysis, they concluded that the disease is caused by an as-yet-uncultured and uncharacterized actinomycete, which they named Tropheryma whippelii.…”

Section: Taxonomy and Microbiologic Aspectsmentioning

confidence: 99%

The medically important aerobic actinomycetes: epidemiology and microbiology

1994

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The aerobic actinomycetes are soil-inhabiting microorganisms that occur worldwide. In 1888, Nocard first recognized the pathogenic potential of this group of microorganisms. Since then, several aerobic actinomycetes have been a major source of interest for the commercial drug industry and have proved to be extremely useful microorganisms for producing novel antimicrobial agents. They have also been well known as potential veterinary pathogens affecting many different animal species. The medically important aerobic actinomycetes may cause significant morbidity and mortality, in particular in highly susceptible severely immunocompromised patients, including transplant recipients and patients infected with human immunodeficiency virus. However, the diagnosis of these infections may be difficult, and effective antimicrobial therapy may be complicated by antimicrobial resistance. The taxonomy of these microorganisms has been problematic. In recent revisions of their classification, new pathogenic species have been recognized. The development of additional and more reliable diagnostic tests and of a standardized method for antimicrobial susceptibility testing and the application of molecular techniques for the diagnosis and subtyping of these microorganisms are needed to better diagnose and treat infected patients and to identify effective control measures for these unusual pathogens. We review the epidemiology and microbiology of the major medically important aerobic actinomycetes.

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“…The elucidation of the cause of new and emerging infectious diseases is more challenging and often even impossible when using only conventional methods. The shift from phenotypic to molecular methods has largely improved the identification process and facilitated the detection of yet unknown microorganisms (Relman et al, 1992;Roads et al, 2012). The final product of this ongoing evolution in diagnostics is the implementation of mass spectrometry for fast and accurate identification of microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Molecular typing of bacteria for epidemiological surveillance and outbreak investigation / Molekulare Typisierung von Bakterien für die epidemiologische Überwachung und Ausbruchsabklärung

Ruppitsch

2016

Die Bodenkultur: Journal of Land Management, Food and Environment

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SummaryConstant confrontations with microbial threats pose major challenges to human and animal health, agricultural and food production, and public safety. Identifying pathogenic bacteria (species) and tracking strains (by series of well-characterized isolates) to their sources are especially important in outbreak investigations. Compared to the identification of the species, the identification of the source and spread of microbial infections represents a major-and many times futile-challenge. This is due to the multitude of ways microorganisms can occur and spread within healthcare facilities and in the community; how, when, and where they can contaminate the complex nutrition chain, leading to natural and man-made outbreaks. Typing is the characterization of isolates or strains below species or subspecies level. Typing of bacterial isolates is an essential procedure to identify the microbe causing the illness or to track down an outbreak to the suspected source. In the genomic era, the introduction of molecular methods has largely replaced phenotypic methods and "molecular epidemiology" has emerged as a new discipline. The current molecular typing methods can be classified into three categories: (a) PCR-based methods, (b) DNA fragment analysis-based methods, and (c) DNA sequence-based methods, including the new exciting era of high-throughput genome sequencing. Keywords

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Identification of the Uncultured Bacillus of Whipple’s Disease

Cited by 1,095 publications

References 36 publications

Maladie immunoproliférative de l'intestin grêle associée à Campylobacter jejuni

Maladie immunoproliférative de l'intestin grêle associée à Campylobacter jejuni

The medically important aerobic actinomycetes: epidemiology and microbiology

Molecular typing of bacteria for epidemiological surveillance and outbreak investigation / Molekulare Typisierung von Bakterien für die epidemiologische Überwachung und Ausbruchsabklärung

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