Identification of thioketone analogues of sildenfil using gas chromatography–mass spectrometry

Simultaneous determination of 38 phosphodiestrase-5 inhibitors in illicit erectile dysfunction products by liquid chromatography–electrospray ionization-tandem mass spectrometry

“…Meanwhile, analogues of thio-sildeanfil produced the Table 1 Overview of the molecular formula, retention times and experimental parameters for the analysis of 38 target compounds by sMRM ESI-positive ionization mode. common ions at m/z 299, which correspond to the cleavage of C N bond and sequential loss of the ethyl group on the ethoxy substituent on the phenyl ring [16]. In addition, an ion at m/z 297 was observed to be the most common ion, which losses of the ethyl, ethyl piperazine and carbonyl groups in analouges of hongdenafil [18].…”

“…Song et al presented the flow injection tandem mass spectrometry method (FI-MS/MS) to identify PDE-5 inhibitors and their analogues [15]. A gas chromatography-mass spectrometry (GC-MS) method was used to analyze thioketone analogues of sildenafil [16]. Although 24 PDE-5 inhibitors and their analogues were analyzed using LC-MS/MS [3], almost all previous studies focused on specific compounds such as sildenafil, tadalafil, and vardenafil.…”

“…Meanwhile, several authors reported the screening and confirmation method of synthetic PDE-5 inhibitors in dietary supplements [9][10][11][12][13][14][15][16]. The ultra-high-performance liquid chromatography (UHPLC)-UV method for nine ED drugs and some of their analogues was developed and validated [9].…”

Fluorescence polarisation for high‐throughput screening of adulterated food products via phosphodiesterase 5 inhibition assay

“…At present, several analytical methods have been utilised to determine PDE5 inhibitors in various matrices. [13][14][15][16] More commonly, liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) has proven to be invaluable in the analysis of PDE5 inhibitors. 17,18 However, LC-MS/MS, in general, demands higher operational and maintenance costs with limited sample throughput.…”

Identification and evaluation of fragmentation pathways of PDE-5 inhibitor analogues using LC-QTOF-MS

“…A number of analytical techniques have been developed for the detection and determination of PDE-5 inhibitors, such as immunoassays, 16 atomic emission spectrometry, 17 ion mobility spectrometry, 18,19 micro-Raman spectroscopy, 20 high-performance liquid chromatography (HPLC) with ultraviolet or fluorescence detection, 21-23 gas chromatography-mass spectrometry (GC-MS), 24,25 and liquid chromatography-mass spectrometry (LC-MS). [26][27][28][29][30][31] Liquid chromatographyquadrupole-time of flight mass spectrometry (LC-QTOF-MS) carried out in full scan mode can be used to elucidate the accurate mass of target and nontarget compounds, as well as that of unknown compounds, while the multiple reaction monitoring (MRM) mode can be used only to detect the mass of target compounds.…”