Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans: Expression analysis in deltamethrin susceptible and resistant populations

Grosso, Carla Gisela; Blariza, María José; Mougabure‐Cueto, Gastón; Picollo, María Inés; Garcia, Beatríz A.

doi:10.1016/j.meegid.2016.07.027

Cited by 33 publications

(51 citation statements)

References 40 publications

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“…They found that the CYP4 gene analyzed was significantly overexpressed in resistant individuals to pyrethroids. Overexpression of a CYP4 gene in a resistant T. infestans population was reported (Grosso et al, 2016), suggesting that this could be an expanded mechanism of pyrethroid resistance in this species. Overall, our assay opens possibilities for functional studies on triatomine detoxification mechanisms.…”

Section: Discussionmentioning

confidence: 96%

NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

et al. 2018

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Although having five different ways of transmission the vector-borne is the principal way of transmission of Chagas disease, which involves insects of the subfamily Triatominae (Hemiptera: Reduviidae). Nineteen of the 31 species that occur in Mexico are associated with humans, and all are capable of transmitting the disease. Pyrethroids are the insecticides recommended for the control of these vectors in Mexico. We determined the susceptibility to the pyrethroids deltamethrin and permethrin of peridomestic populations of Triatoma mazzottii Usinger and two populations of Triatoma longipennis Usinger in comparison with a reference strain for each species. Bioassays were performed for the determination of the LD for both field populations and reference strains. A maximum of 27 fold resistance to deltamethrin was observed in T. mazzottii, meanwhile, for permethrin, T. longipennis from Jalisco show the highest value of 3.19 fold. There was significantly increased activity of esterases in field populations in comparison with their corresponding reference strain. The results of the search of kdr mutations related to the resistance to deltamethrin and permethrin in the evaluated species show the presence of mutations in the field populations, as is the case with individuals of T. mazzottii, for which the mutation was found A943V, and for the two populations of T. longipennis included in this study, we report the presence of the kdr mutation K964R. Evaluation of the various mechanisms involved in resistance to pyrethroids in triatomines from Mexico could guide us to the real justification for insecticide resistance monitoring.

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Section: Discussionmentioning

confidence: 96%

NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

et al. 2018

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“…These data provide the evidence on the circadian control of insect resistance to insecticides. 34 Because it was also detected that overexpression of a cytochrome P450 (CYP4EM7) gene in a highly insecticideresistant strain of T. infestans, the clock gene PER could be also involved in the circadian control of the metabolic pathways of insecticide detoxification and resistance. 35 This is the first work related to the circadian clock in T. infestans.…”

Section: Discussionmentioning

confidence: 99%

Clock Gene Period in the Chagas Disease Vector Triatoma infestans (Hemiptera: Reduviidae)

Stroppa

Gíménez

Garcia

2018

The American Journal of Tropical Medicine and Hygiene

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To contribute to a better understanding of the molecular bases of the circadian biological rhythms in Chagas disease vectors, in this work we identified functional domains in the sequences of the clock protein PERIOD (PER) in and and analyzed the expression of the PER gene at mRNA level in . The PER protein sequences comparison among these species and those from other insects revealed that the most similar regions are the PAS domains and the most variable is the COOH-terminal. On the other hand, the gene expression in nervous tissue of adult varies with a daily canonical rhythm in groups of individuals maintained under photoperiod (light/dark, LD) and constant dark (DD), showing a significant peak of expression at sunset. The pattern of expression detected in LD persists under the DD condition. As expected, in the group maintained in constant light (LL), no daily increase was detected in transcript level. Besides, the presence of transcript in different tissues of adult individuals and in nervous tissue of nymphs evidenced activity of peripheral clocks in adults and activity of the central clock in nymphs of.

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“…Many authors have demonstrated that the P450 monooxygenases are involved in the metabolism of virtually all insecticides, leading to activation of the molecule in the case of organophosphorus insecticides, or more generally to detoxification (Hemingway and Ranson 2000). As new research is being developed in this topic, the complete sequencing of this enzymatic group is being characterized, which would allow for the development of new markers to detect individual enzymes involved in the resistance mechanism in each population (Grosso et al 2016; Ibrahim et al 2015). …”

Section: Discussionmentioning

confidence: 99%

Toxicological, Enzymatic, and Molecular Assessment of the Insecticide Susceptibility Profile ofTriatoma infestans(Hemiptera: Reduviidae, Triatominae) Populations From Rural Communities of Santa Cruz, Bolivia

Santo-Orihuela¹,

Vassena

Carvajal³

et al. 2016

J Med Entomol

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A wide range of insecticide resistance profiles has been reported across Bolivian domestic and sylvatic populations of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae), including some with levels proven to be a threat for vector control. In this work, the insecticide profile of domestic T. infestans was studied with standardized toxicological bioassays, in an area that has not undergone consistent vector control. F1 first-instarnymphs hatched in laboratory from bugs captured in three communities from the Santa Cruz Department were evaluated with different insecticides. Moreover, the enzymatic activity of esterases and cytochrome P450 monooxygenases was measured in individual insects to evaluate the possible mechanism of metabolic resistance to pyrethroids. In addition, the DNA sequence of sodium channel gene (kdr) was screened for two point mutations associated with pyrethroid resistance previously reported in T. infestans. All populations showed reduced susceptibility to deltamethrin and α-cypermethrin, albeit the RR50 values varied significantly among them. Increased P450 monooxygenases and permethrate esterases suggest the contribution, as detoxifying mechanisms, to the observed resistance to deltamethrin in all studied populations. No individuals presented either mutation associated to resistance in the kdr gene. The level of susceptibility to α-cypermethrin, the insecticide used by the local vector control program, falls within an acceptable range to continue its use in these populations. However, the observed RR50 values evidence the possibility of selection for resistance to pyrethroids, especially to deltamethrin. Consequently, the use of pyrethroid insecticides should be closely monitored in these communities, which should be kept under entomological surveillance and sustained interventions.

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Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans: Expression analysis in deltamethrin susceptible and resistant populations

Cited by 33 publications

References 40 publications

NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

NovelKdrmutations (K964R and A943V) in pyrethroid‐resistant populations ofTriatoma mazzottiiandTriatoma longipennisfrom Mexico and detoxifying enzymes

Clock Gene Period in the Chagas Disease Vector Triatoma infestans (Hemiptera: Reduviidae)

Toxicological, Enzymatic, and Molecular Assessment of the Insecticide Susceptibility Profile ofTriatoma infestans(Hemiptera: Reduviidae, Triatominae) Populations From Rural Communities of Santa Cruz, Bolivia

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