Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma

Jin, Donghui; Song, Yuxuan; Chen, Yuan; Zhang, Peng

doi:10.1155/2020/7573689

Cited by 27 publications

(25 citation statements)

References 27 publications

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“…These backgrounds suggest the possibility that lncRNAs may be involved in paracrine signaling or cell-to-cell crosstalk. Our findings indicate that LINC01234 downregulation could suppress hepatic carcinoma cell proliferation and induce apoptosis, which is consistent with earlier reports indicating that lncRNAs regulate the progression of liver cancer (23,24). These findings suggest that LINC01234 likely acts to promote tumorigenesis of liver cancer, particularly during the advanced stages of the disease.…”

Section: Discussionsupporting

confidence: 93%

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

Song

et al. 2020

Preprint

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Background Liver cancer is a frequent malignancy with poor prognosis. It has been reported that many lncRNAs could regulate the progression of liver cancer. To identify potential therapeutic targets for liver cancer, we conducted bioinformatics analysis of lncRNAs in tumor tissues and adjacent normal tissues. Methods The differential expression of lncRNAs between liver cancer tissues and adjacent normal tissues were examined by bioinformatics analysis. Cell proliferation was tested by CCK-8. Cell apoptosis in liver cancer was detected by flow cytometry. Gene and protein expression in liver cancer cells were measured by q-PCR and Western-blot, respectively. Xenograft tumor model was established to verify the function of LNC01234 on liver cancer in vivo. Results LNC01234 was found to be notably upregulated in liver cancer tissues. In addition, knockdown of LNC01234 significantly inhibited the proliferation, invasion and induced the apoptosis of liver cancer cells. Meanwhile, miR-513a-5p was a downstream target of LNC01234 and USP4 was a direct target of miR-513a-5p. Moreover, downregulation of LNC01234 inhibited the tumorigenesis of liver cancer via inactivating TGF-β signaling. Conclusion Downregulation of LNC01234 could inhibit the progression of liver cancer, which may serve as a potential novel target for treatment of liver cancer.

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Section: Discussionsupporting

confidence: 93%

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

Song

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…These backgrounds suggest the possibility that lncRNAs may be involved in paracrine signaling or cell-to-cell crosstalk. Our ndings indicate that LINC01234 downregulation could suppress hepatic carcinoma cell proliferation and induce apoptosis, which is consistent with earlier reports indicating that lncRNAs regulate the progression of liver cancer (23,24). These ndings suggest that LINC01234 likely acts to promote tumorigenesis of liver cancer, particularly during the advanced stages of the disease.…”

Section: Discussionsupporting

confidence: 92%

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

Song

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Liver cancer is a frequent malignancy with poor prognosis. It has been reported that many lncRNAs could regulate the progression of liver cancer. To identify potential therapeutic targets for liver cancer, we conducted bioinformatics analysis of lncRNAs in tumor tissues and adjacent normal tissues. Methods: The differential expression of lncRNAs between liver cancer tissues and adjacent normal tissues were examined by bioinformatics analysis. Cell proliferation was tested by CCK-8. Cell apoptosis in liver cancer was detected by flow cytometry. Gene and protein expression in liver cancer cells were measured by q-PCR and Western-blot, respectively. Xenograft tumor model was established to verify the function of LINC01234 on liver cancer in vivo . Results: LINC01234 was found to be notably upregulated in liver cancer tissues. In addition, knockdown of LINC01234 significantly inhibited the proliferation, invasion and induced the apoptosis of liver cancer cells. Meanwhile, miR-513a-5p was a downstream target of LINC01234 and USP4 was a direct target of miR-513a-5p. Moreover, downregulation of LINC01234 inhibited the tumorigenesis of liver cancer via inactivating TGF-β signaling. Conclusion: Downregulation of LINC01234 could inhibit the progression of liver cancer. Thus, LINC01234 may serve as a potential novel target for treatment of liver cancer.

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“…Moreover, overexpressed AURKA might promote hepatocellular carcinoma cell growth, adhesion and migration in vitro (28). RACGAP1 has been identified as a hub gene and significantly associated with overall survival in patients with various cancer types, such as pancreatic ductal adenocarcinoma (29), gastric cancer (30), cervical cancer (31) and lung adenocarcinoma (32). Furthermore, Zhao has found that depletion of RACGAP1 could lead to mitotic catastrophe and massive cell death in esophageal squamous cell carcinoma (33).…”

Section: Discussionmentioning

confidence: 99%

A Novel Five-Gene Signature for Prognosis Prediction in Hepatocellular Carcinoma

Zhang

Kong

2021

Front. Oncol.

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Hepatocellular carcinoma (HCC) has been a global health issue and attracted wide attention due to its high incidence and poor outcomes. In this study, our purpose was to explore an effective prognostic marker for HCC. Five cohort profile datasets from GEO (GSE25097, GSE36376, GSE62232, GSE76427 and GSE101685) were integrated with TCGA-LIHC and GTEx dataset to identify differentially expressed genes (DEGs) between normal and cancer tissues in HCC patients, then 5 upregulated differentially expressed genes and 32 downregulated DEGs were identified as common DEGs in total. Next, we systematically explored the relationship between the expression of 37 common DEGs in tumor tissues and overall survival (OS) rate of HCC patients in TCGA and constructed a novel prognostic model composed of five genes (AURKA, PZP, RACGAP1, ACOT12 and LCAT). Furthermore, the predicted performance of the five-gene signature was verified in ICGC and another independent clinical samples cohort, and the results demonstrated that the signature performed well in predicting the OS rate of patients with HCC. What is more, the signature was an independent hazard factor for HCC patients when considering other clinical factors in the three cohorts. Finally, we found the signature was significantly associated with HCC immune microenvironment. In conclusion, the prognostic five-gene signature identified in our present study could efficiently classify patients with HCC into subgroups with low and high risk of longer overall survival time and help clinicians make decisions for individualized treatment.

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Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma

Cited by 27 publications

References 27 publications

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

A Novel Five-Gene Signature for Prognosis Prediction in Hepatocellular Carcinoma

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