Identification of Three Strains of a Virus Associated with Cassava Plants Affected by Frogskin Disease

Calvert, Lee A.; Cuervo, Maritza; Lozano, Iván; Villareal, Natalia; Arroyave, José A.

doi:10.1111/j.1439-0434.2008.01412.x

Cited by 26 publications

(25 citation statements)

References 17 publications

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“…These primers amplify a fragment of 720 nucleotides (nt) of the RNA‐dependent RNA polymerase (RdRp) domain. The presence of co‐infecting viruses in the samples was checked by RT‐PCR using available primers for torradoviruses (Verbeek et al ., ) and for the Cassava frogskin‐associated virus (CsFSaV) reovirus (Calvert et al ., ). Fifteen PCR amplicons were cloned using the pGEM‐T Easy vector (Promega) system and sent for sequencing (Macrogen).…”

Section: Methodsmentioning

confidence: 97%

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cuellar

Celli

et al. 2018

Plant Pathology

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Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT‐PCR and sequencing. In this work, the virus has been successfully purified and a double‐antibody sandwich (DAS‐) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co‐infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.

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Section: Methodsmentioning

confidence: 97%

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cuellar

Celli

et al. 2018

Plant Pathology

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“…It was cloned and sequenced, with the finding that a virus from the Reoviridae family (reo-like viruses) was infecting the cassava. This virus is now associated with the disease (Calvert et al, 2008).…”

Section: Etiologymentioning

confidence: 99%

Cassava Genetic Improvement

Ceballos¹,

Fregene²,

Carlos³

et al. 2007

Breeding Major Food Staples

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PREFACEThis Handbook is the result of a combined effort by several current and previous cassava researchers at CIAT to review and summarize the most important results of cassava research during the past 40 years. Most of the chapters are based on the various presentations during the Regional Cassava Training Course, held in Thailand from October 6 to 17, 2008. This course was organized upon the realization that many people that were actively involved with cassava research in the 1970s and 80s, both at CIAT and in national programs, have now retired or will soon do so, and that a whole new generation of cassava researchers are currently being hired to take their place.As cassava is now becoming a very important, and mostly industrial, crop in Asia, there are many new opportunities, but also a host of new problems and challenges. These include the appearance in Asia of new cassava diseases and pests; the decreasing availability and increasing cost of rural labor, resulting in the need for partial or complete mechanization of cassava production; the rapidly increasing demand for cassava roots for production of food, feed and fuel, and the unavailability in many countries of new land for any expansion of cassava area, thus requiring a rapid increase in cassava yields to increase supplies. This requires a renewed focus on cassava research for the development of new higher-yielding varieties and more sustainable production practices.While many cassava researchers in national programs in Asia received individual or group training at CIAT-Colombia during the 1970s and 80s, this training was greatly reduced during the following two decades due to funding limitations. Thus, the objective of the Regional Cassava Training Course in 2008 was to provide a new training opportunity for young scientists in Asian countries, and to hand over the knowledge and experience of the older cassava researchers, mostly from CIAT, to a new generation that will have to face the new challenges. Thus, the course was taught mostly by current or already retired CIAT cassava researchers, while the 60-plus participants of the course included cassava researchers from Cambodia, China, East Timor, India, Indonesia, Lao PDR, Malaysia, Myanmar, the Philippines, Thailand and Vietnam. The training course not only provided knowledge -from physiology and biotechnology to animal feeding and production of fuelethanol -but also an opportunity for people from different institutions and countries to get to know each other, which will greatly facilitate future collaboration.Course participants returned home with a CD containing the PowerPoint presentations of the course. However, it was felt that a more comprehensive review of all the topics covered was warranted, as this would provide more in-depth knowledge for those working in the various specialized fields. Most of this information is available in many refereed journals, in workshop proceedings and old CIAT annual reports, but many of these are now out of print or otherwise difficult to obtain. Thus, the Cas...

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“…The frogskin disease is one of the main limiting factors for cassava production in Colombia as it directly affects the roots, causing losses in yield up to 90% (Álvarez et al 2009;Carvajal-Yepes et al 2014). In addition, it has been reported in Brazil, Costa Rica, Panama, Perú and Venezuela (Oliveira et al 2014; de Souza et al 2014; Carvajal-Yepes et al 2014) and has been associated with both virus (Pineda & Lozano, 1981;Calvert et al 2008), and phytoplasmas (16SrIII-L) (Álvarez et al 2003; 2009).…”

Section: Introductionmentioning

confidence: 99%

Aislamiento de fitoplasmas asociados a cuero de sapo en yuca

Betancourth

Pardo

Flórez

et al. 2019

Rev. U.D.C.A Act. & Div. Cient.

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Frogskin is the most limiting disease of cassava crops in Colombia, causing losses in production up to 90%. Since this disease was associatated with 16SrIII phytoplasma presence, a study was carried out to isolate this phytoplasma using liquid and solid culture media. Root, petiol, stem, leaf and cutting tissues of cassava affected by frogsking were employed as source materials. Molecular and microscopy techniques were applied to verify the phytoplasma growth and to discard other microorganism´s presence. The results showed that the media consistently allow phytoplasma growth, and colonies in solid medium were obtained. PCR, qPCR and sequencing tests confi rmed the presence of 16SrIII group phytoplasmas in both liquid and solid culture media. Additionally, the isolation of a pigeon pea witches' broom phytoplasma strain (group 16SrIX) was obtained from stems, petioles and fl owers of symptomatic Catharanthus roseus confi rming the effectiveness of the medium in the phytoplasma isolation and culture. This is the fi rst isolation of fi eld-collected phytoplasma strains in groups 16SrIII and 16SrIX in America that confi rm and corroborate the previous results in phytoplasma cultivation achieved on micropropagated and fi eldcollected phytoplasma infected samples.

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Identification of Three Strains of a Virus Associated with Cassava Plants Affected by Frogskin Disease

Cited by 26 publications

References 17 publications

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cassava Genetic Improvement

Aislamiento de fitoplasmas asociados a cuero de sapo en yuca

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