Identification of TIFY gene family in walnut and analysis of its expression under abiotic stresses

Abstract

Background Walnuts (Juglans regia L.) are known for their nutrient-rich nuts and are one of the important economic tree species in the world. However, due to global warming and soil salinization, walnuts suffer from various abiotic stresses. TIFY (TIF[F/Y]XG) proteins play an essential role in the growth and development of plants, signal transduction, and stress response in plants. At present, although the TIFY gene family of a number of plants has been identified and studied, how TIFY takes part in stress tolerance remains obscure and many functions of TIFY require further investigation. Result In this study, twenty-one TIFY transcription factors were identified in the walnut genome database, and they were divided into four subfamilies (TIFY, JAZ, ZML, and PPD) by bioinformatics analysis. Chromosome location revealed tandem duplication of some genes. Phylogenetic tree analysis showed JrTIFYs were closely related to the TIFY gene family of Arabidopsis thaliana (A. thaliana). qRT-PCR (quantitative real-time PCR) analysis revealed the TIFY genes have different expression patterns in ‘Qingxiang’ and ‘Xiangling’ walnut varieties under drought, heat, and salt stress. JAZ subfamily was more expressed in different abiotic stress than other subfamilies. The expressions of JrTIFY14 under heat and salt stress were significantly higher than those under drought stress. However, the expression of JrTIFYs was not significant in ‘Xiangling’. Conclusion This study reveals the TIFY gene family plays an important role in walnuts facing abiotic stresses and provides a theoretical basis for walnut breeding.

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

“…This is consistent with previous studies done on walnuts, where JrTIFY14 of the JAZ subfamily positively regulated heat and salt stress in both cultivars. The study also confirmed that different tolerance walnut varieties have different Tify gene expression patterns, leading to drought, high‐temperature and salt stress resistance (Liu et al, 2022a).…”

Genome-wide identification and expression of TIFY family in cassava (Manihot esculenta Crantz)

“…Several other genes in monocots are most likely compensating for the molecular activities done by PPD genes ( Bai et al., 2011 ). Cassava, like other dicot plants, such as Populus trichocarpa , walnut ( Juglans regia ), and peach ( Prunus persica ), has members of all four subfamilies based on domain traits ( Wang et al., 2017 ; Liu et al., 2022 ; Sheng et al., 2022 ).…”

“…Overexpression of maize JAZ14 in Arabidopsis may improve seedling tolerance to hormone treatments such as ABA and JA, as well as polyethylene glycol stress ( Zhou et al., 2015 ). Under salt stress, in soybean ( Glycine max ), GmTIFY10e and GmTIFY10g transgenic plants grew faster and had longer root lengths and fresh weights than wild-type plants ( Liu et al., 2022 ). Similarly, virtually all TIFY genes in rice are susceptible to a variety of abiotic stresses, and OsTIFY11a overexpression resulted in a significant increase in tolerance to salt and dehydration stress ( Ye et al., 2009 ).…”

Insights into the regulation of wild soybean tolerance to salt-alkaline stress

“…The TIFY family was divided into TIFY , Jasmonate ZIM (JAZ), ZIM-like (ZML), and PEAPOD (PPD) subfamilies. A previous genome-wide analysis identified 34 TIFY genes in G. soja ( Zhu et al., 2013 ), and a recent study reported 38 TIFY genes in G. max ( Liu X. et al., 2022 ). In G. soja , GsTIFY10s (10a, 10b, 10c, 10d, 10e, and 10f) and GsTIFY11s (11a and 11b) shared the highest sequence identity and were clustered together in the JAZ subfamily.…”