Identification of Toxoplasma Gondii in Lymph-Nodes by Immunofluorescence

Demonstration of parasites in toxoplasma lymphadenitis by fine‐needle aspiration cytology: Report of two cases

“…[16][17][18] Organisms in toxoplasma lymphadenitis have been reported infrequently. [19][20][21] There are no more than 6 cases of toxoplasma cysts reported in excisional lymph node biopsies, in 2 of which cytologic sampling of the specimen (touch imprint in one and FNA in another) helped in identifying the cysts. [22][23][24][25] Case 1 of the present report is the only one in the literature in which a toxoplasma cyst was recovered by direct FNA of a patient's lesion.…”

“…27 Furthermore, diagnosis of the disease in tissue preparations by demonstration of parasites or antigen in cells by the indirect immunofluorescence method, using toxoplasma-specific antibody, has been reported. 21 Immunocytochemical staining with T. gondii antibody is valuable in localizing toxoplasma antigen in organisms or cells of the inflammatory response which have neutralized such organisms. 28 This should be performed preferably on slides with adequate material, which have been fixed but not previously stained routinely or with other methods, as destaining and additional handling of such preparations can result in elimination of most of the antigen from the material.…”