Nuclear pairing gaps of normally deformed and superdeformed nuclei are investigated using the particle-number conserving (PNC) formalism for the cranked shell model, in which the blocking effects are treated exactly. Both rotational frequency ω-dependence and seniority (number of unpaired particles) ν-dependence of the pairing gap∆ are investigated. For the ground-state bands of even-even nuclei, PNC calculations show that in general∆ decreases with increasing ω, but the ω-dependence is much weaker than that calculated by the number-projected Hartree-FockBogolyubov approach. For the multiquasiparticle bands (seniority ν > 2), the pairing gaps keep almost ω-independent. As a function of the seniority ν, the bandhead pairing gaps∆(ν, ω = 0) decrease slowly with increasing ν. Even for the highest seniority ν bands identified so far,∆(ν, ω = 0) remains greater than 70% of∆(ν = 0, ω = 0).