Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX

The KRAB Domain of Zinc Finger Gene ZNF268: a Potential Transcriptional Repressor

“…The two isoforms had different expression pattern. ZNF268-1 was expressed in normal tissues, whereas ZNF268-2 was expressed in several tumor cell lines and in all tested CLL cases (30). The deletion of KRAB domain in ZNF268 might affect the function of ZNF268 like that ZNF74 acted as a transcriptional repressor with the fulllength KRAB domain but lacked repressor activity with a truncated KRAB domain (31).…”

HLA homozygosity and haplotype bias among patients with chronic lymphocytic leukemia: implications for disease control by physiological immune surveillance

“…Genetic markers such as FLT3 internal tandem duplications (FLT3-ITD), were first identified in adults 1 and subsequently in children, 2 as conferring a poor prognosis. By contrast, mutations in nucleophosmin (NPM1) 3,4 and more recently, CCAAT/enhancer-binding protein alpha (CEBPA) 5 have been found to be associated with improved outcomes in both adult and pediatric studies. However, despite being initially described almost 20 years ago, the prognostic implications of mutations involving the RAS proto-oncogenes, remain controversial.…”

“…Nonetheless, there is much evidence that the immune system can be induced to recognize CLL, including the existence of a graft versus leukemia effect after allogeneic hematopoietic stem cell transplantation, the documentation of a disease-specific antigenic profile, and the demonstration of de novo immune responses following experimental vaccination protocols. [3][4][5] In this study, we demonstrate that CLL patients with advanced disease exhibit bias toward homozygosity at human leukocyte antigen (HLA) loci, limiting the diversity of antigen presentation and potentially providing the nascent tumor with an advantage in the process of immune escape. This is the first report to correlate the clinical progression of CLL with HLA homozygosity, demonstrating that immune surveillance might have a role in the containment of non-infectious neoplasms.…”

Tumor-associated Antigen Arrays for the Serological Diagnosis of Cancer

“…This transactivation is facilitated by the binding of LEDGF/p75 to heat shock elements and stress-related elements in promoter regions of target genes (88). LEDGF/p75 has been also identified as a target autoantigen in leukemia by serological analysis of recombinant expression libraries (SEREX) (89). Consistent with this finding, the LEDGF gene is involved in chromosomal translocations in patients with various types of leukemia, resulting in a fusion with nucleoprotein-98 that retains the C terminus of LEDGF/p75 (90 -92).…”