Identification of Tumor Microenvironment-Related Alternative Splicing Events to Predict the Prognosis of Endometrial Cancer

Abstract

BackgroundEndometrial cancer (EC) is one of the most common female malignant tumors. The immunity is believed to be associated with EC patients’ survival, and growing studies have shown that aberrant alternative splicing (AS) might contribute to the progression of cancers.MethodsWe downloaded the clinical information and mRNA expression profiles of 542 tumor tissues and 23 normal tissues from The Cancer Genome Atlas (TCGA) database. ESTIMATE algorithm was carried out on each EC sample, and the OS-related different expressed AS (DEAS) events were identified by comparing the high and low stromal/immune scores groups. Next, we constructed a risk score model to predict the prognosis of EC patients. Finally, we used unsupervised cluster analysis to compare the relationship between prognosis and tumor immune microenvironment.ResultsThe prognostic risk score model was constructed based on 16 OS-related DEAS events finally identified, and then we found that compared with high-risk group the OS in the low-risk group was notably better. Furthermore, according to the results of unsupervised cluster analysis, we found that the better the prognosis, the higher the patient’s ESTIMATE score and the higher the infiltration of immune cells.ConclusionsWe used bioinformatics to construct a gene signature to predict the prognosis of patients with EC. The gene signature was combined with tumor microenvironment (TME) and AS events, which allowed a deeper understanding of the immune status of EC patients, and also provided new insights for clinical patients with EC.

Comprehensive characterization of 11 prognostic alternative splicing events in ovarian cancer interacted with the immune microenvironment

“…The prognostic values of AS events have been reported in patients with glioblastoma, breast cancer, lung cancer, and so on 18 . The correlation between survival-associated AS events and the immune microenvironment have also been confirmed in endometrial cancer, stomach adenocarcinoma, pancreatic cancer and so on 12 , 19 , 20 . Furthermore, dysregulated splicing factors (SFs) have been reported to cause global alteration of AS events in cancer 3 .…”

The Correlation Between Novel Survival-related Alternative Splicing Signature and Prognostic Prediction and Immune Microenvironment in Clear Cell Renal Cell Carcinoma

“…These studies demonstrate that AS events can play a very important role in the TIME. What's more, the relationship between AS events and TIME has been con rmed in a variety of tumor diseases, such as hepatocellular carcinoma [46], esophageal squamous cell carcinoma [47], gastric carcinoma [48], endometrial carcinoma [49], triple negative breast carcinoma [50] and glioma [51]. However, this relationship has not been studied inccRCC.…”

Survival-associated 11-AS Events Interact with the Immune Microenvironment in Ovarian Cancer

“…The prognostic values of AS events have been reported in patients with glioblastoma, breast cancer, lung cancer, and so on 14 . The correlation between survivalassociated AS events and the immune microenvironment have also been con rmed in endometrial cancer, stomach adenocarcinoma, pancreatic cancer and so on 9,15,16 . Furthermore, dysregulated splicing factors (SFs) have been reported to cause global alteration of AS events in cancer 3 .…”