Identification of tuna species by a real-time polymerase chain reaction technique

ELISA-based detection of mislabeled albacore (Thunnus alalunga) fresh and frozen fish fillets

“…Species misdescription at this level should be prevented, since fresh and frozen albacore are considered by fish experts as one of the best choices for many high-quality recipes (Chuang, Chen, & Shiao, 2012;Michelini et al, 2007). Results obtained using the indirect ELISA described showed 13 samples (32.5%), out of the 40 fish samples analysed, incorrectly assigned (negative values) in the fish market, all of them frozen samples.…”

“…In fact, DNA methods based on PCR technology have been successfully developed for the rapid identification of Scombridae species in raw and processed samples (Chuang, Chen, & Shiao, 2012;Michelini et al, 2007;Pardo & Pérez-Villareal, 2004;Quinteiro et al, 1998;Ram, Ram, & Baidoun, 1996;Rehbein et al, 1999). These include, for example, PCR followed by phylogenetic analysis for the authentication of scombrid species using the mitochondrial cytochrome b and cytochrome c oxidase I as molecular marker (Cawthorn, Steinman, & Witthuhn, 2011;Espiñeira, Gonzalez-Lavín, Vieites, & Santaclara, 2009); the PCR-restriction fragment length polymorphism (PCR-RFLP) (Aguilar, Alonso, & Barrero, 2012;Lin & Hwang, 2007); or the real-time PCR (Chuang, Chen, & Shiao, 2012).…”

“…These include, for example, PCR followed by phylogenetic analysis for the authentication of scombrid species using the mitochondrial cytochrome b and cytochrome c oxidase I as molecular marker (Cawthorn, Steinman, & Witthuhn, 2011;Espiñeira, Gonzalez-Lavín, Vieites, & Santaclara, 2009); the PCR-restriction fragment length polymorphism (PCR-RFLP) (Aguilar, Alonso, & Barrero, 2012;Lin & Hwang, 2007); or the real-time PCR (Chuang, Chen, & Shiao, 2012).…”

“…These methods have shown considerable value in certain instances, but they are not convenient for routine sample analyses because they are relatively costly, time-consuming and complex to perform. Consequently, in the last years the identification of fish and seafood, as well as meat, milk and other food products has been performed preferentially by genetic (Armani, Castigliego, Tinacci, Gianfaldoni, & Guidi, 2012;Carrera et al, 1998;Chuang, Chen, & Shiao, 2012;Dalmasso et al, 2007;Espiñeira, Gonzalez-Lavín, Vieites, & Santaclara, 2009;Pardo & Pérez-Villareal, 2004) and immunological techniques (Carrera et al, 1996).…”

Modern analytical methods for the detection of food fraud and adulteration by food category

“…This part of the review will be limited to discussing the PCR‐based methods for the authentication of numerous species of fish and seafood (Table ). The identification of fish species in tinned tuna and sardine cans has been successfully carried out using PCR analysis . In particular, there is a need to detect fraud related to sturgeon caviar, one of the most exclusive and expensive fishery products, and PCR‐RFLP analysis provided a powerful and easy method for the identification of mislabeled caviar .…”