Identification of tuna species by computer-assisted and cluster analysis of PCR–SSCP electrophoretic patterns

Colombo, Fabio; Mangiagalli, Gerard; Renon, P

doi:10.1016/j.foodcont.2003.11.006

Cited by 21 publications

(14 citation statements)

References 6 publications

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“…Number References PCR-RLFP 71 [1, 3, 4, 6, 8, 9, 21, 23, 24, 26, 28-31, 35, 36, 38, 39, 44, 46-48, 50, 52, 54, 57-66, 70, 73, 76-78, 80, 84-88, 91, 92, 96, 100, 104-108, 112, 113, 120, 123-125, 127, 130, 133, 134, 139, 145-147, 150, 151] PCR-sequencing 43 [2, 12, 15, 17, 18, 40, 43, 45, 46, 48, 51, 53, 56, 63-67, 70, 71, 73-75, 79, 81, 82, 94, 102, 104, 107, 115, 116, 118, 124, 126, 129, 132, 136, 137, 140, 142, 148, 149] PCR-specific primers 23 [5,7,9,11,14,27,33,34,40,50,68,69,89,95,97,98,100,117,122,127,128,138,141] PCR-SSCP 9 [10,32,37,39,[109][110][111][112]144] Real-time PCR 9 [25,41,49,55,70,90,135,138,143] PCR-RAPD 5 [19,20,…”

Section: Pcr-sequencingmentioning

confidence: 99%

Molecular identification methods of fish species: reassessment and possible applications

Teletchea

2009

Rev Fish Biol Fisheries

257

192

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Fish species identification is traditionally based on external morphological features. Yet, in many cases fishes and especially their diverse developmental stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative. This work intends to provide an updated and extensive overview on the PCR-methods for fish species identification. Among the ten main methods developed, three PCR-RFLP, PCR-FINS and PCR-specific primers have been the most used. Two other emerging methods, namely real-time PCR and microarray technology, offer new potential for quantification of DNA and simultaneous detection of numerous species, respectively. Almost 500 species have been targeted in the past decade, among which the most studied belong to gadoids, scombroids, and salmonids. The mitochondrial cytochrome b gene was by far the most targeted DNA markers. The most common applications belonged to the forensic, taxonomic, and ecological fields. At last, some key problems, such as the degradation of DNA, the reliability of sequences, and the use of scientific names, likely to be encountered during the development of molecular identification methods are described. In conclusion, the tremendous advances in molecular biology in the past 10 years has rendered possible the study of DNA from virtually any substrates, offering new perspectives for the development of various applications, which will likely continue to increase in the future.

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Section: Pcr-sequencingmentioning

confidence: 99%

Molecular identification methods of fish species: reassessment and possible applications

Teletchea

2009

Rev Fish Biol Fisheries

257

192

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“…[7,8] Nevertheless, fragment size is a limiting factor for the subsequent PCR reaction, which is based on the selective amplification of specific regions of DNA using oligonucleotides. [8] PCR [9][10][11] and its modifications-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), [12][13][14][15] Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), [16,17] real-time PCR, [18][19][20][21] and Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) [22] -represent crucial approaches for tuna fish species identification. These approaches comprise DNA extraction from the sample, PCR, and electrophoresis, or alternatively other detection systems for the evaluation of the final results.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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The most common techniques used to identify tuna species include methods based on the detection of specific DNA. The quality of species-specific DNA crucially affects the efficiency of amplification during the subsequent polymerase chain reaction (PCR). Detection of DNA in processed products can be adversely affected by DNA fragmentation during the processing steps and the use of ingredients that may inhibit the PCR reaction. In this study, several processing treatments applied to the muscle tissue of yellowfin tuna (Thunnus albacares) were evaluated. For DNA isolation three DNA extraction methods were compared. The concentration, purity, and amplificability of DNA were tested. The results revealed the variability among extraction procedures in terms of DNA quality and quantity in tuna muscle tissue processed under different processing technologies. ARTICLE HISTORY

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“…1 was analysed by 1D Kodak 2.0 Image Analysis Software and the mobility values of the PCR products of each sample (matrix 9 Â 3), considered as variables for multivariate statistical analysis, were subjected to cluster analysis with Statistica software. A similar approach was also used by Colombo, Mangiagalli, and Renon (2005) to analyse SSCP electrophoretic patterns to tuna speciation. Fig.…”

Section: Species-specific Dna Amplificationmentioning

confidence: 99%

Identification of the species of origin of milk in cheeses by multivariate statistical analysis of polymerase chain reaction electrophoretic patterns

Santos

Fernandes

Bardsley

2012

International Dairy Journal

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Identification of tuna species by computer-assisted and cluster analysis of PCR–SSCP electrophoretic patterns

Cited by 21 publications

References 6 publications

Molecular identification methods of fish species: reassessment and possible applications

Molecular identification methods of fish species: reassessment and possible applications

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Identification of the species of origin of milk in cheeses by multivariate statistical analysis of polymerase chain reaction electrophoretic patterns

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