Identification of Two 5α-Reduced Pregnanes as Major Metabolites of Progesterone in Immature Rat Ovaries (1000 × g supernatant)<i>in Vitro</i>

Lerner, Nicole; Eckstein, B.

doi:10.1210/endo-98-1-179

Cited by 35 publications

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“…Isolation and identification of transformation products The procedures for the isolation and identification of progesterone, 3a-hydroxy-5a-pregnan-20-one (3a-P), 3a,17a-dihydroxy-5a-pregnan-20-one (3 ,17 -), and of 5a-androstane-3a,17/?-diol (3a-A) matched those recently described by Lerner & Eckstein (1976). As can be seen from Table 1, PMSG treatment resulted, in both concentrations of substrate employed, in the appearance of a major metabolite which was not detected in the controls.…”

Section: Resultsmentioning

confidence: 93%

Pre-Ovulatory Changes in Steroidogenesis in Ovaries From Immature Rats Treated With Pregnant Mare Serum Gonadotrophin

Eckstein¹,

Lerner²,

Yehud³

1976

Journal of Endocrinology

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The metabolism of progesterone in the 1000 g supernatant fraction of homogenates of ovaries from PMSG-treated immature rats was determined. As early as 52 h after a single injection of 50 i.u. PMSG, still before the LH surge, 5alpha-pregnane-3alpha, 20alpha-diol was identified as the main metabolite, together with small quantities of 3alpha-hydroxy-kalpha-pregnan-20-one and 5alpha-androstane-3alpha, 17beta-diol. Similar incubations of untreated rat ovaries at the same age did not produce 5alpha-pregnane-3alpha, 20alpha-diol. The quantities of 3alpha-hydroxy-5alpha-pregnan-20-one and 5alpha-androstane-3alpha, 1mbeta-diol were reduced in PMSG-treated rat ovaries as compared with control ovaries. When progesterone metabolism was examined 64 h after PMSG administration, 5-7 h after the peak of LH surge but still before ovulation, 75% of the substrate was converted to 5alpha-pregnane-3alpha, 20alpha-diol, while 3alpha-hydroxy-5alpha-pregnan-20-one as well as 5alpha-androstane-3alpha, 17beta-diol could not be detected.

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Section: Resultsmentioning

confidence: 93%

Pre-Ovulatory Changes in Steroidogenesis in Ovaries From Immature Rats Treated With Pregnant Mare Serum Gonadotrophin

Eckstein¹,

Lerner²,

Yehud³

1976

Journal of Endocrinology

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“…Similar to the pharmacologic group of benzodiazepines, which also act on the GABA A receptor complex, these steroids have anesthetic effects (Stephenson et al 1986). The metabolites can also be produced within the female genital tract and ovary of mammals in vitro (Lerner and Eckstein 1976;Lisboa and Holtermann 1976). Sperm also appear to possess steroidogenic potential (Nagahama 1987).…”

Section: Discussionmentioning

confidence: 99%

Sperm acrosome antigen-1

Breuckmann

Brucker

2002

Cell and Tissue Research

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The acrosome reaction is a fundamental requirement for mammalian fertilization. Its exact molecular mechanisms and cellular elements are still poorly understood. We have detected an acrosomal sperm antigen, SAA-1, by monoclonal antibodies directed against SAA-1, that appears to be critically involved in the regulation of the acrosome reaction. SAA-1 is conserved within a broad range of mammalian species, emphasizing its important role in mammalian reproduction. Here we demonstrate that SAA-1 is immunohistochemically detectable in a nonmammalian vertebrate whose sperm do not possess an acrosome. In the swordtail, a live-bearing teleost with special reproductive tactics, we were able to demonstrate immunoreactivity of sperm heads of spermatids and mature sperm in the testis using monoclonal antibodies against SAA-1. Due to the cystic spermatogenesis with synchronous sperm maturation, immunoreactive maturational stages could be clearly identified. Stored immunoreactive sperm were also identified in spermathecal tissue of the female genital tract. Interestingly, immunoreactivity was furthermore detected in defined cells of the compounded endocrine organs pituitary and endocrine pancreas. All these different cell systems are involved in paracrine regulation and exhibit exocytotic properties. The possible nature of SAA-1 is discussed. Additionally, some new aspects of the morphologic composition of the swordtail pituitary are described.

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Changes in ovarian 5α-steroid reductase and 20α-hydroxysteroid dehydrogenase activity produced by induction of first ovulation with gonadotropin

Eckstein

Lerner

1977

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Identification of Two 5α-Reduced Pregnanes as Major Metabolites of Progesterone in Immature Rat Ovaries (1000 × g supernatant)in Vitro

Cited by 35 publications

References 0 publications

Pre-Ovulatory Changes in Steroidogenesis in Ovaries From Immature Rats Treated With Pregnant Mare Serum Gonadotrophin

Pre-Ovulatory Changes in Steroidogenesis in Ovaries From Immature Rats Treated With Pregnant Mare Serum Gonadotrophin

Sperm acrosome antigen-1

Changes in ovarian 5α-steroid reductase and 20α-hydroxysteroid dehydrogenase activity produced by induction of first ovulation with gonadotropin

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