Identification of two AFLP markers linked to bacterial wilt resistance in tomato and conversion to SCAR markers

Abstract

Tomato bacterial wilt (BW) incited by Ralstonia solanacearum is a constraint on tomato production in tropical, subtropical and humid regions of the world. In this paper, we present the results of a research aimed at the identification of PCR-based markers amplified fragment length polymorphism (AFLP) linked to the genes that confer resistance to tomato BW. To this purpose, bulked segregant analysis was applied to an F(2) population segregating for the BW resistant gene and derived from the pair-cross between a BW resistant cultivar T51A and the susceptible cultivar T9230. Genetic analysis indicated that tomato BW was conferred by two incomplete dominant genes. A CTAB method for total DNA extraction, developed by Murray and Thompson with some modifications was used to isolation the infected tomato leaves. Thirteen differential fragments were detected using 256 primer combinations, and two AFLP markers were linked to the BW resistance. Subsequently, the AFLP markers were converted to co-dominant SCAR markers, named TSCAR(AAT/CGA) and TSCAR(AAG/CAT). Linkage analysis showed that the two markers are on the contralateral side of TRSR-1. Genetic distance between TSCAR(AAT/CGA) and TRS-1 was estimated to 4.6 cM, while 8.4 cM between TSCAR(AAG/CAT) and TRS-1.

RETRACTED ARTICLE: A reexamination of molecular markers for use in marker-assisted breeding in tomato

“…In a recent study, molecular markers associated with BW resistance in a highly-resistant cultivar (T51A) were reported (Miao et al 2009). The BW resistance in T51A was conferred by two complementary, co-dominant genes, located on tomato chromosome 6.…”

Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

“…Mapping studies have shown the involvement of generalist as well as strain-specific quantitative trait loci (QTLs) in S. lycopersicum Hawaii 7996 (Carmeille et al 2006;Mangin et al 1999;Thoquet et al 1996;Wang et al 2000). In addition, bulked segregant analysis has shown the presence of two incompletely dominant genes associated with resistance in tomato cultivar T51A (Miao et al 2009). In pepper, the inheritance of BW resistance in a double haploid population derived from the cross C. annuum var.…”

Identification of Gene Related to Hard Bunch Phenotype in Oil Palm (Elaeis guineensis Jacq.)

“…This method was also applied to identify molecular markers that associated with resistance to bacteria in tomato (Miao et al, 2009), and those associated to cytoplasmic male sterility in Brassica napus (Zeng et al, 2009).…”

“…AFLP markers combined with Bulk Segregant Analysis (BSA) method have been widely used to discover marker closely associated with trait in many crops including Brassica napus (Zeng et al, 2009) and tomato (Miao et al, 2009). AFLP markers have been used for analysis of oil palm genome (Barcelos et al, 2002), date palm genome (Rhouma et al, 2007) as well as other crops such as Amorphophallus variabilis (Santosa et al, 2012).…”