Identification of two copies of the gene for the elongation factor EF–Tu in E. Coli

Abstract

Two copies of the structural gene for the elongation factor EF-Tu have been identified in Escherichia coli: one near rif and the other near str. The latter seems to belong to a single transcriptional unit together with the genes for ribosomal protein S7, S12 (str) and the elongation factor EF-G (fus).

Regulation of the expression of tufA and tufB, the two genes coding for the elongation factor EF‐Tu in escherichia coli

“…Its intracellular abundance is surprisingly high [4-71. Escherkhia coli harbours two genes coding for EF-Tu: tufA, located at 73 mm on the E. coli linkage map [8], lies in the so-called str region [9] as the distal-most gene of a 4-gene operon [lo]; tuj73 lies in the rif region near 88 min [9] and is co-transcribed with 4 upstream tRNA genes [ 11,131. The nucleotide sequences of tufA and tu~?B have been determined [14,15].…”

Allosteric changes of the guanine nucleotide site of elongation factor EF‐Tu

“…During the elongation cycle of protein biosynthesis it plays an essential role in the correct placement of aminoacyl-tRNA onto the ribosome-messenger RNA complex [1,2]. In Escherichia coli two genes, tufA and tufB, code for EF-Tu [3][4][5]. The primary structure of the polypeptide chain of these two gene products is identical, only the C-terminal residue is different [6].…”

A kirromycin resistant elongation factor EF‐Tu from Escherichia coli contains a threonine instead of an alanine residue in position 375

“…Two unlinked genes coding for EF-Tu have been identified on the E. coli chromosome [8][9][10]. One designated tufA maps at 72 rain, the other, designated tufB, at 88 min.…”