Identification of Two Genes, sll0804 and slr1306, as Putative Components of the CO
            <sub>2</sub>
            -Concentrating Mechanism in the Cyanobacterium
            <i>Synechocystis</i>
            sp. Strain PCC 6803

Zhang, Shulu; Spann, Kevin W.; Frankel, Laurie K.; Moroney, James V.; Bricker, Terry M.

doi:10.1128/jb.01126-08

Cited by 4 publications

(2 citation statements)

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“…Most of the α-cyanobacteria such as Prochlorococcus and Synechococcus strains inhabit marine while β-cyanobacteria such as Synechocystis sp. PCC 6803 [37] , [38] , [39] , Anabaena variabilis [40] , and Synechococcus elongatus PCC 7942 [41] live mainly in freshwater. The two distinct environments differ mainly in their conditions such as pH, C i content, and salinity.…”

Section: Introductionmentioning

confidence: 99%

Exploring Components of the CO 2 -Concentrating Mechanism in Alkaliphilic Cyanobacteria Through Genome-Based Analysis

Klanchui

Cheevadhanarak

Prommeenate

et al. 2017

Computational and Structural Biotechnology Journal

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In cyanobacteria, the CO2-concentrating mechanism (CCM) is a vital biological process that provides effective photosynthetic CO2 fixation by elevating the CO2 level near the active site of Rubisco. This process enables the adaptation of cyanobacteria to various habitats, particularly in CO2-limited environments. Although CCM of freshwater and marine cyanobacteria are well studied, there is limited information on the CCM of cyanobacteria living under alkaline environments. Here, we aimed to explore the molecular components of CCM in 12 alkaliphilic cyanobacteria through genome-based analysis. These cyanobacteria included 6 moderate alkaliphiles; Pleurocapsa sp. PCC 7327, Synechococcus spp., Cyanobacterium spp., Spirulina subsalsa PCC 9445, and 6 strong alkaliphiles (i.e. Arthrospira spp.). The results showed that both groups belong to β-cyanobacteria based on β-carboxysome shell proteins with form 1B of Rubisco. They also contained standard genes, ccmKLMNO cluster, which is essential for β-carboxysome formation. Most strains did not have the high-affinity Na+/HCO3− symporter SbtA and the medium-affinity ATP-dependent HCO3− transporter BCT1. Specifically, all strong alkaliphiles appeared to lack BCT1. Beside the transport systems, carboxysomal β-CA, CcaA, was absent in all alkaliphiles, except for three moderate alkaliphiles: Pleurocapsa sp. PCC 7327, Cyanobacteriumstranieri PCC 7202, and Spirulina subsalsa PCC 9445. Furthermore, comparative analysis of the CCM components among freshwater, marine, and alkaliphilic β-cyanobacteria revealed that the basic molecular components of the CCM in the alkaliphilic cyanobacteria seemed to share more degrees of similarity with freshwater than marine cyanobacteria. These findings provide a relationship between the CCM components of cyanobacteria and their habitats.

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Section: Introductionmentioning

confidence: 99%

Exploring Components of the CO 2 -Concentrating Mechanism in Alkaliphilic Cyanobacteria Through Genome-Based Analysis

Klanchui

Cheevadhanarak

Prommeenate

et al. 2017

Computational and Structural Biotechnology Journal

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“…Earlier, we have used this technique to identify previously undescribed components of the cyanobacterial carbon-concentrating mechanism (19) and have also elucidated a differential role for the malic enzyme in carbon metabolism under continuous versus diurnal illumination (20). In the current study, a cyanobac-terial mutant (designated Tsll0606) bearing a transposon insertion in the sll0606 gene was isolated.…”

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confidence: 99%

The Sll0606 Protein Is Required for Photosystem II Assembly/Stability in the Cyanobacterium Synechocystis sp. PCC 6803

Zhang

Frankel

Bricker

2010

Journal of Biological Chemistry

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An insertional transposon mutation in the sll0606 gene was found to lead to a loss of photoautotrophy but not photoheterotrophy in the cyanobacterium Synechocystis sp. PCC 6803. Complementation analysis of this mutant (Tsll0606) indicated that an intact sll0606 gene could fully restore photoautotrophic growth. Gene organization in the vicinity of sll0606 indicates that it is not contained in an operon. No electron transport activity was detected in Tsll0606 using water as an electron donor and 2,6-dichlorobenzoquinone as an electron acceptor, indicating that Photosystem II (PS II) was defective. Electron transport activity using dichlorophenol indolephenol plus ascorbate as an electron donor to methyl viologen, however, was the same as observed in the control strain. This indicated that electron flow through Photosystem I was normal. Fluorescence induction and decay parameters verified that Photosystem II was highly compromised. The quantum yield for energy trapping by Photosystem II (F V /F M ) in the mutant was less than 10% of that observed in the control strain. The small variable fluorescence yield observed after a single saturating flash exhibited aberrant Q A ؊ reoxidation kinetics that were insensitive to dichloromethylurea. Immunological analysis indicated that whereas the D2 and CP47 proteins were modestly affected, the D1 and CP43 components were dramatically reduced. Analysis of twodimensional blue native/lithium dodecyl sulfate-polyacrylamide gels indicated that no intact PS II monomer or dimers were observed in the mutant. The CP43-less PS II monomer did accumulate to detectable levels. Our results indicate that the Sll0606 protein is required for the assembly/stability of a functionally competent Photosystem II.In higher plants, algae, and cyanobacteria, at least six intrinsic proteins appear to be required for oxygen evolution by PS II 2 (1-3). These are CP47, CP43, the D1 and D2 proteins, and the ␣ and ␤ subunits of cytochrome b 559 . Insertional inactivation or deletion of the genes for these components results in the absence of PS II complex assembly and the complete loss of oxygen evolution activity (for a review, see Ref. 4). For maximal rates of oxygen evolution in cyanobacteria, the extrinsic proteins PsbO, PsbU, PsbV, and PsbQ must also be present (5). Additionally, a large number of other intrinsic membrane components are present in PS II complexes (6 -8), although the functions of many of these proteins remain obscure. The most recent crystal structure of the thermophilic cyanobacterium Thermosynechococcus elongatus (9) indicates that PS II contains 20 protein components (it should be noted that PsbQ, which is essential for maximum PS II activity in cyanobacteria (10), is missing from the current crystal structure).PS II assembly and turnover requires a variety of other protein components (for a comprehensive review, see Ref. 11). Although many of these proteins are conserved in all oxygenic organisms, a subset is present only in the cyanobacteria. These include the Synechocystis sp. PC...

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Sulfur metabolism in cyanobacteria

Karvansara,

Komenda,

Kopriva

2024

Cyanobacteria

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Identification of Two Genes, sll0804 and slr1306, as Putative Components of the CO ₂ -Concentrating Mechanism in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Cited by 4 publications

References 20 publications

Exploring Components of the CO 2 -Concentrating Mechanism in Alkaliphilic Cyanobacteria Through Genome-Based Analysis

Exploring Components of the CO 2 -Concentrating Mechanism in Alkaliphilic Cyanobacteria Through Genome-Based Analysis

The Sll0606 Protein Is Required for Photosystem II Assembly/Stability in the Cyanobacterium Synechocystis sp. PCC 6803

Sulfur metabolism in cyanobacteria

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Identification of Two Genes, sll0804 and slr1306, as Putative Components of the CO 2 -Concentrating Mechanism in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Cited by 4 publications

References 20 publications

Exploring Components of the CO 2 -Concentrating Mechanism in Alkaliphilic Cyanobacteria Through Genome-Based Analysis

Exploring Components of the CO 2 -Concentrating Mechanism in Alkaliphilic Cyanobacteria Through Genome-Based Analysis

The Sll0606 Protein Is Required for Photosystem II Assembly/Stability in the Cyanobacterium Synechocystis sp. PCC 6803

Sulfur metabolism in cyanobacteria

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Identification of Two Genes, sll0804 and slr1306, as Putative Components of the CO ₂ -Concentrating Mechanism in the Cyanobacterium Synechocystis sp. Strain PCC 6803