Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori

Matsumoto, Hitoshi; Ueno, Chihiro; Nakamura, Yuki; Kinjoh, Terunori; Ito, Yuka; Shimura, Sachiko; Noda, Hiroaki; Imanishi, Shigeo; Mita, Kazuei; Fujiwara, Haruhiko; Hiruma, Kiyoshi; Shinoda, Tetsuro; Kamimura, Manabu

doi:10.1016/j.jinsphys.2015.02.011

Cited by 4 publications

(3 citation statements)

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“…We also examined the expression profiles of E75 and EcR, both of which are known as ecdysone response genes in several insect species and are well understood in D. melanogaster [41] and B. mori [42,43]. The transcript levels of the E75 common region and EcR were high in male Pu stage (Fig 4A and 4G), suggesting that ecdysteroid titer is high during male adult development.…”

Section: Discussionmentioning

confidence: 99%

Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)

et al. 2020

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Insect molting hormone (ecdysteroids) and juvenile hormone regulate molting and metamorphic events in a variety of insect species. Mealybugs undergo sexually dimorphic metamorphosis: males develop into winged adults through non-feeding, pupa-like stages called prepupa and pupa, while females emerge as neotenic wingless adults. We previously demonstrated, in the Japanese mealybug Planococcus kraunhiae (Kuwana), that the juvenile hormone titer is higher in males than in females at the end of the juvenile stage, which suggests that juvenile hormone may regulate male-specific adult morphogenesis. Here, we examined the involvement of ecdysteroids in sexually dimorphic metamorphosis. To estimate ecdysteroid titers, quantitative RT-PCR analyses of four Halloween genes encoding for cytochrome P450 monooxygenases in ecdysteroid biosynthesis, i.e., spook, disembodied, shadow and shade, were performed. Overall, their expression levels peaked before each nymphal molt. Transcript levels of spook, disembodied and shadow, genes that catalyze the steps in ecdysteroid biosynthesis in the prothoracic gland, were higher in males from the middle of the second nymphal instar to adult emergence. In contrast, the expression of shade, which was reported to be involved in the conversion of ecdysone into 20-hydroxyecdysone in peripheral tissues, was similar between males and females. These results suggest that ecdysteroid biosynthesis in the prothoracic gland is more active in males than in females, although the final conversion into 20-hydroxyecdysone occurs at similar levels in both sexes. Moreover, expression profiles of ecdysone response genes, ecdysone receptor and ecdysone-induced protein 75B, were also analyzed. Based on these expression profiles, we propose that the changes in ecdysteroid titer differ between males and females, and that high ecdysteroid titer is essential for directing male adult development.

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Section: Discussionmentioning

confidence: 99%

Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)

et al. 2020

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“…Their presence during the extended feeding stage may also indicate that factor(s) other than ecdysone play a role in the expression of these genes. Previous studies report that the application of another juvenile hormone analogue, methoprene, induces the expression of some E74 isoforms in the midguts of A. aegypti (Wu et al, 2006) and expression of E75A in Drosophila S2 cells (Dubrovsky et al, 2004), larval haemocytes of Bombyx mori (Matsumoto et al, 2015) and the 5 th larval instar epidermis of Manduca sexta (Zhou et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Juvenile hormone analogue, fenoxycarb, modulates ecdysone-triggered transcriptional hierarchy during programmed cell death of midgut in silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Göncü¹,

Uranli²,

Parlak³

2017

Eur. J. Entomol.

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“…RhoprE75 (un gen de respuesta a ecdisona) y RhoprDis (Disembodied), implicado en su biosíntesis (Zhou, Hiruma et al 1998;Bialecki, Shilton et al 2002;Dubrovsky, Dubrovskaya et al 2004;Wang, Wang et al 2012;Matsumoto, Ueno et al 2015). El gen E75 es un factor de transcripción que posee múltiples isoformas en insectos (A-E), siendo las isoformas A y B las más conservadas (Jindra, Sehnal et al 1994;Palli, Ladd et al 1997;Zhou, Hiruma et al 1998).…”

Section: Efecto De Dsoks Sobre Genes Relacionados Con La Síntesis Y Lunclassified

Genómica, proteómica y fisiología de neuropéptidos en el insecto triatomino Rhodnius prolixus

Wulff¹

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En Argentina existen aproximadamente 2.300.000 infectados de Chagas. El control del insecto vector continúa siendo la opción más viable como control y prevención de la enfermedad. En las últimas dos décadas se detectó la existencia de poblaciones de triatominos resistentes a insecticidas (Triatoma infestans), en las provincias de Chaco, Salta, y en el Sur de Bolivia. Esto representa un grave riesgo para el control de la enfermedad de Chagas en Argentina y la región. El presente trabajo utiliza como organismo modelo al triatomino R. prolixus. Este insecto reúne una serie de características favorables para su uso como modelo en fisiología de insectos: es el segundo vector más importante de la enfermedad de Chagas; posee características favorables para su cría; se conoce su secuencia genómica; los resultados obtenidos en R. prolixus potencialmente pueden aplicarse a otros triatominos, en incluso otros insectos. Particularmente, para estudios de desarrollo post-embrionario como los realizados en este trabajo, una ventaja del modelo es que el ciclo de muda se desencadena de manera predecible tras la ingesta de sangre, lo que permite sincronizar los experimentos. Este trabajo de Tesis se inscribe en el marco de alcanzar alternativas de control de insectos perjudiciales, que resulten más eficientes, selectivas y seguras para el medio ambiente respecto a los insecticidas neurotóxicos en uso. En éste sentido, se estudian los neuropéptidos, hormonas peptídicas que regulan todos los procesos vitales en organismos multicelulares. El estudio de estas moléculas tiene potencial aplicabilidad para el diseño de insecticidas. Podría permitir el diseño de pseudo-péptidos o péptido-miméticos capaces de interferir con la fisiología de insectos de importancia sanitaria o económica, constituyendo una vía novedosa hacia la obtención de nuevos insecticidas.

show abstract

Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori

Cited by 4 publications

References 58 publications

Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)

Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)

Juvenile hormone analogue, fenoxycarb, modulates ecdysone-triggered transcriptional hierarchy during programmed cell death of midgut in silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Genómica, proteómica y fisiología de neuropéptidos en el insecto triatomino Rhodnius prolixus

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