“…The TIR domain, which is functionally important for TLR signal transduction, contains three active motifs: box 1 (FDAFISY), box 2 (LC-RD-PG), and box 3 (a conserved W surrounded by basic residues) [34]. It has been suggested that box 1 and box 2 mediate binding to receptor molecules of signaling transduction pathways, while box 3 has been shown to be primarily involved in directing localization of receptors [34]. These active motifs were basically conserved in CiTLR22 (box 1, Y 802 DAFISY 808 ; box 2, L 832 C-RD-PG 842 and box 3, W 934 surrounded by basic residue K940), and TLR22 of other fish species.…”