Identification of two mutant JASON-RELATED genes associated with unreduced pollen production in potato

Clot, Corentin R.; Vexler, Lea; de La O Leyva-Perez, Maria; Bourke, Peter M.; Engelen, Christel J. M.; Hutten, Ronald C. B.; van de Belt, José; Wijnker, Erik; Milbourne, Dan; Visser, Richard G. F.; Juranić, Martina; van Eck, Herman J.

doi:10.1007/s00122-024-04563-7

Cited by 3 publications

(1 citation statement)

References 79 publications

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“…Unreduced gametes are necessary for heritable introgression. Contemporary breeders have made extensive use of such crosses to introduce novel traits ( Ortiz et al., 1994 ; Capo et al., 2002 ; Zimnoch-Guzowska and Flis, 2021 ; Clot et al., 2023 , 2024 ) and it seems likely that this sort of introgression has been a tool used by humans throughout potato’s history ( Hoopes et al., 2022 ; Meng et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Potato soup: analysis of cultivated potato gene bank populations reveals high diversity and little structure

Tuttle,

Del Rio,

Bamberg

et al. 2024

Front. Plant Sci.

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Cultivated potatoes are incredibly diverse, ranging from diploid to pentaploid and encompass four different species. They are adapted to disparate environments and conditions and carry unique alleles for resistance to pests and pathogens. Describing how diversity is partitioned within and among these populations is essential to understanding the potato genome and effectively utilizing landraces in breeding. This task is complicated by the difficulty of making comparisons across cytotypes and extensive admixture within section petota. We genotyped 730 accessions from the US Potato genebank including wild diploids and cultivated diploids and tetraploids using Genotype-by-sequencing. This data set allowed us to interrogate population structure and diversity as well as generate core subsets which will support breeders in efficiently screening genebank material for biotic and abiotic stress resistance alleles. We found that even controlling for ploidy, tetraploid material exhibited higher observed and expected heterozygosity than diploid accessions. In particular group chilotanum material was the most heterozygous and the only taxa not to exhibit any inbreeding. This may in part be because group chilotanum has a history of introgression not just from wild species, but landraces as well. All group chilotanum, exhibits introgression from group andigenum except clones from Southern South America near its origin, where the two groups are not highly differentiated. Moving north, we do not observe evidence for the same level of admixture back into group andigenum. This suggests that extensive history of admixture is a particular characteristic of chilotanum.

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Section: Discussionmentioning

confidence: 99%

Potato soup: analysis of cultivated potato gene bank populations reveals high diversity and little structure

Tuttle,

Del Rio,

Bamberg

et al. 2024

Front. Plant Sci.

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A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids

Addo Nyarko,

Katche,

Báez

et al. 2024

Heredity

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The establishment of successful interspecies hybrids requires restoration of a stable “2n” chromosome complement which can produce viable “n” gametes. This may occur (rarely) via recombination between non-homologous chromosomes, or more commonly is associated with a doubling of parental chromosome number to produce new homologous pairing partners in the hybrid. The production of unreduced “2n” gametes (gametes with the somatic chromosome number) may therefore be evolutionarily useful by serving as a key pathway for the formation of new polyploid hybrids, as might specific mechanisms permitting recombination between non-homologous chromosomes. Here, we investigated chromosome complements and fertility in third generation interspecific hybrids (AABC) resulting from a cross between allopolyploids Brassica juncea (AABB) × B. napus (AACC) followed by self-pollination for two generations. Chromosome numbers ranged from 2n = 48–74 in the experimental population (35 plants), with 9–16 B genome chromosomes and up to 4 copies of A genome chromosomes. Unreduced gamete production leading to a putative genome structure of approximately AAAABBCC was hence predicted to explain the high chromosome numbers observed. Additionally, the estimation of nuclei number in post-meiotic sporads revealed a higher frequency of unreduced gametes (0.04–5.21%) in the third generation AABC interspecific hybrids compared to the parental Brassica juncea (0.07%) and B. napus (0.13%). Our results suggest that unreduced gamete production in the subsequent generations following interspecific hybridization events may play a critical role in restoration of more stable, fertile chromosome complements.

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QTL discovery for agronomic and quality traits in diploid potato clones using PotatoMASH amplicon sequencing

Vexler,

Leyva-Perez,

Konkolewska

et al. 2024

G3: Genes, Genomes, Genetics

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We genotyped a population of 618 diploid potato clones derived from six independent potato-breeding programmes from NW-Europe. The diploids were phenotyped for 23 traits, using standardised protocols and common check varieties, enabling us to derive whole population estimators for most traits. We subsequently performed a Genome-Wide Association Study (GWAS) to identify quantitative trait loci (QTL) for all traits with SNPs and short-read haplotypes derived from read-backed phasing. In this study, we used a marker platform called PotatoMASH (Potato Multi-Allele Scanning Haplotags); a pooled multiplex amplicon sequencing based approach. Through this method, neighbouring SNPs within an amplicon can be combined to generate multi-allelic short-read haplotypes (haplotags) that capture recombination history between the constituent SNPs, and reflect the allelic diversity of a given locus in a different way than single bi-allelic SNPs. We found a total of 37 unique QTL across both marker types. A core of 10 QTL were detected with SNPs as well as with haplotags. Haplotags allowed to detect an additional 14 QTL not found based on the SNP set. Conversely, the bi-allelic SNP set also found 13 QTL not detectable using the haplotag set. We conclude that both marker types should routinely be used in parallel to maximize the QTL detection power. We report 19 novel QTL for nine traits: Skin Smoothness, Sprout Dormancy, Total Tuber Number, Tuber Length, Yield, Chipping Colour, After-cooking Blackening, Cooking Type and Eye depth.

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Identification of two mutant JASON-RELATED genes associated with unreduced pollen production in potato

Cited by 3 publications

References 79 publications

Potato soup: analysis of cultivated potato gene bank populations reveals high diversity and little structure

Potato soup: analysis of cultivated potato gene bank populations reveals high diversity and little structure

A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids

QTL discovery for agronomic and quality traits in diploid potato clones using PotatoMASH amplicon sequencing

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