Identification of Two New Races of Podosphaera xanthii Causing Powdery Mildew in Melon in South Korea

Hong, ye-ji; Hossain, Mohammad Rashed; Kim, Hoy‐Taek; Park, Jong-In; Nou, Ill–Sup

doi:10.5423/ppj.oa.12.2017.0261

Cited by 29 publications

(20 citation statements)

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“…However, this approach has not yet been fully adopted in cucurbit breeding because of gaps in our understanding of virulence variation in CPM populations (Lebeda et al, 2018a). The classical approach to racial denomination of both Go and Px (Hong et al, 2018;Lebeda et al, 2011;Lebeda & Sedláková, 2006;McCreight, 2006;Yuste-Lisbona et al, 2010) was inefficient and often hampered direct comparisons of results obtained by different research groups (Lebeda et al, 2016a(Lebeda et al, , 2016b.…”

Section: Discussionmentioning

confidence: 99%

“…However, a new, unified system for CPM racial determination and denomination was published in 2016 (Lebeda et al, 2016a). That set of CPM differentials included 21 C. melo accessions, establishing an opportunity for better international communication in evaluation of CPM virulence variation by replacing hundreds of classically described races (v-phenotypes), based on disparate differential sets and systems for racial description (e.g., Hong et al, 2018;Lebeda et al, 2011;Lebeda & Sedláková, 2006;McCreight, 2006;Yuste-Lisbona et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Spatiotemporal changes in the geographic distribution of both Go and Px have been observed during the last three decades (Křístková et al, 2009;Lebeda et al, 2007bLebeda et al, , 2009Pirondi et al, 2015). Significant variation in virulence, expressed by numerous pathotypes and/or races and their spatiotemporal fluctuations, has been described for both species (Bardin et al, 1999;Cohen et al, 2004;Hong et al, 2018;Lebeda et al, 2007aLebeda et al, , 2008Lebeda et al, , 2009Lebeda & Sedláková, 2006;McCreight, 2006). The fact that both pathogens can develop simultaneous infections in a single crop (Křístková et al, 2009;Lebeda et al, 2009) is often overlooked (Lebeda et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

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Understanding pathogen population structure and virulence variation for efficient resistance breeding to control cucurbit powdery mildews

et al. 2021

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Cucurbit powdery mildew (CPM) is caused most frequently by well-differentiated obligate erysiphaceous ectoparasites Golovinomyces orontii and Podosphaera xanthii, which vary in their ecology and virulence. All economically important cucurbit crops host both of these CPM species. Breeding of cucurbits for CPM resistance is highly important worldwide, but adequate knowledge of CPM species determination, as well as virulence structure, population dynamics, and spatiotemporal variation of these pathogens, has not yet been achieved. New tools have been developed to enhance research on CPM virulence variation for more efficient breeding and seed and crop production. A set of differential genotypes of Cucumis melo, with high differentiation capacity, may contribute substantially to understanding of variation in CPM virulence at both individual and population levels. Long-term observations (2001-2012) of CPM pathogens in the Czech Republic were used to analyse virulence variation within and among annual CPM populations and demonstrate the utility of recently developed tools for studying species variability and virulence variation of CPM pathogens worldwide. Detailed analyses of diversity and spatiotemporal fluctuations in the composition of CPM populations provide crucial information for shaping breeding programmes and predicting the most effective sources of race-specific resistance. The primary aim of this work was to create a uniform framework for determination of CPM species structure and diversity, virulence phenotypes, virulence and phenotype frequencies, phenotype complexity, dynamics, and variation within and among CPM populations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Understanding pathogen population structure and virulence variation for efficient resistance breeding to control cucurbit powdery mildews

et al. 2021

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“…Previous studies characterized races 1, N1, N2, and 5 that cause powdery mildew in melon (Hosoya et al 1999) and later, seven different races (race 1, 2, 3, 4, 5, 6, and 7) has been reported (Kuzuya et al 2004) followed by a new race, N5 in Japan (Kim et al 2016b). Recently, we reported two new races, KN1 and KN2 that cause serious disease on melon in South Korea (Hong et al 2018;Kim et al 2016a;Kim et al 2016b). Despite moderate success in controlling P. xanthii by agrochemicals, cost effectiveness, development of pathogenic resistance to fungicides due to long term use and ecological hazard is of great concern.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of Resistance to Race 5 of Powdery Mildew fungus Podosphaera xanthii in Melon and development of Race 5-Specific High Resolution Melting Markers

Hong

Hossain

Jung

et al. 2022

Preprint

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Powdery mildew (PM), caused by the biotrophic fungus Podosphaera xanthii, drastically reduces the yield and quality of melon (Cucumis melo L.). Knowledge on the genetic control and high throughput molecular markers linked with resistance against this disease are essential for breeding programs. The bioassay study of the F1 and F2 populations derived from the parents, ‘PMR 5’ (♂) and ‘SCNU1154’ (♀) revealed a monogenic dominant nature of resistance to the devastating race, race 5. Besides, we developed three SNP based high resolution melting markers, PMm-HRM-1, PMm-HRM-2, and PMm-HRM-3 based on the previously identified SNPs on chromosome 12 and validated those using 10 melon lines and 137 F2 population. Among these, the SNP of marker PMm-HRM-1 causes a missense mutation (Lysine → Glutamic acid) in the LRR region of MELO3C002393 and were able to distinguish the resistant vs susceptible genotypes from 10 diverse melon population and the segregating F2 population with more than 90% genotyping efficiency. Other two markers were based on intergenic SNPs and had more than 80% genotyping efficiency F2 population. These markers will be helpful to melon breeders to develop melon cultivars resistant to P. xanthii race 5 via marker assisted breeding programs.

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“…For example, powdery mildew disease caused by Podosphaera xanthii (Schlechtend.:Fr.) Pollacci is a serious threat to melon plants in Japan [4] and in many parts of the world [5][6][7][8][9][10][11][12]. Moreover, powdery mildew fungi are the primary causes of agricultural problems that significantly reduce melon productivity and yield [4,6,10,11,13].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Blue Light-Emitting Diode Irradiation on Podosphaera xanthii Conidial Release and Infection of Melon Seedlings

et al. 2022

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Powdery mildew fungi infect plant leaves, reducing the yield of infected melon plants. Therefore, an eco-friendly method of controlling powdery mildew in melon plants needs to be developed. A previous study described how the morphological characteristics of the conidiophores of the melon powdery mildew fungus Podosphaera xanthii Pollacci (designated KMP-6N) grown under greenhouse (natural) conditions and red light-emitting diode (LED) irradiation differed from those grown under growth chamber conditions and blue LED irradiation. In the present study, conidiophores with unconstricted conidia under blue light were collected and inoculated onto host leaves through micromanipulation; the unconstricted conidia germinated and infected the leaves, producing vigorously elongated hyphae. The number of conidia collected, the initial times of conidial release from single colonies, and the number of conidia remaining in chains on conidiophores were examined with electrostatic techniques. Under red light, the number of collected conidia gradually increased with the light irradiation period. The initial conidial release occurred between 2 to 4 h; the number of conidia remaining on the conidiophores gradually decreased and, eventually, the conidiophore lengths became shorter. In contrast, under blue light, few conidia were collected at any given time; the number of conidia on the conidiophores gradually increased and, eventually, the conidiophore lengths became longer. Next, the effects of red and blue light on the spread of powdery mildew infection by placing a KMP-6N-infected melon seedling at the centre of a tray containing healthy melon seedlings were examined. Almost all healthy seedlings caused powdery mildew symptoms at ca. 21 days after red light irradiation, whereas only healthy seedlings near the infected seedlings showed symptoms after blue light irradiation. Thus, the spread of melon powdery mildew infection clearly differed between red and blue light irradiation. This is the first report describing the effects of red and blue light on the spread of P. xanthii infection from a single infected seedling to healthy host seedlings; their results provide insight into the ecological mechanisms of powdery mildew conidial scatter from conidiophores.

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Identification of Two New Races of Podosphaera xanthii Causing Powdery Mildew in Melon in South Korea

Cited by 29 publications

References 29 publications

Understanding pathogen population structure and virulence variation for efficient resistance breeding to control cucurbit powdery mildews

Understanding pathogen population structure and virulence variation for efficient resistance breeding to control cucurbit powdery mildews

Inheritance of Resistance to Race 5 of Powdery Mildew fungus Podosphaera xanthii in Melon and development of Race 5-Specific High Resolution Melting Markers

Inhibitory Effects of Blue Light-Emitting Diode Irradiation on Podosphaera xanthii Conidial Release and Infection of Melon Seedlings

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