Identification of two phospholipid hydroperoxide glutathione peroxidase (gpx4) genes in common carp

Hermesz, Edit; Ferencz, Ágnes

doi:10.1016/j.cbpc.2009.03.007

Cited by 30 publications

(20 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data, in combination, indicate that the relatively high complement of unsaturated fatty acids in this species may be protected and/or repaired by one or more unmeasured antioxidants (either low molecular weight antioxidants like -tocopherol or other antioxidant enzymes) or PLA 2 . Because longhorn sculpin are a relatively derived marine teleost (Kontula and Väinölä, 2003), and duplicate GPx4 genes have been reported in teleosts (Thisse et al, 2003;Hermesz and Ferencz, 2009), there also exists the possibility that a novel GPx isozyme (undetected by our methods) may be occupying the role typically associated with GPx4. In light of the relationship between membrane unsaturation and GPx4, future studies may consider how the relatively high levels of highly oxidizable phospholipids of sculpin are protected against oxidation.…”

Section: Discussionmentioning

confidence: 96%

“…As a result, these animals may require additional protection from LPO. Expression of two GPx4 genes (gpx4a/b) has been reported during the time course of development in zebrafish (Thisse et al, 2003), following acute exposure to both cold temperature and cadmium in carp (Hermesz and Ferencz, 2009), and in various tissues from farmed southern bluefin tuna (Thompson et al, 2010). In spite of the growing interest in GPx4 of fish, thus far no studies have systematically examined the protein levels or enzymatic activity of GPx4 in their tissues.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between oxidizable fatty acid content and level of antioxidant glutathione peroxidases in marine fish

Grim

Hyndman

Kriska

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYBiological membranes can be protected from lipid peroxidation by antioxidant enzymes including catalase (CAT) and seleniumdependent glutathione peroxidases 1 and 4 (GPx1 and GPx4). Unlike GPx1, GPx4 can directly detoxify lipid hydroperoxides in membranes without prior action of phospholipase A 2 . We hypothesized that (1) GPx4 is enhanced in species that contain elevated levels of highly oxidizable polyunsaturated fatty acids (PUFA) and (2) activities of antioxidant enzymes are prioritized to meet species-specific oxidative stresses. In this study we examined (i) activities of the oxidative enzyme citrate synthase (CS) and antioxidant (CAT, GPx1 and GPx4) enzymes, (ii) GPx4 protein expression, and (iii) phospholipid composition in livers of five species of marine fish (Myxine glutinosa, Petromyzon marinus, Squalus acanthias, Fundulus heteroclitus and Myoxocephalus octodecemspinosus) that contain a range of PUFA. GPx4 activity was, on average, 5.8 times higher in F. heteroclitus and S. acanthias than in the other three marine fish species sampled. Similarly, activities of CAT and GPx1 were highest in S. acanthias and F. heteroclitus, respectively. GPx4 activity for all species correlates with membrane unsaturation, as well as oxidative activity as indicated by CS. These data support our hypothesis that GPx4 level in marine fish is a function, at least in part, of high PUFA content in these animals. GPx1 activity was also correlated with membrane unsaturation, indicating that marine species partition resources among glutathione-dependent defenses for protection from the initial oxidative insult (e.g. H 2 O 2 ) and to repair damaged lipids within biological membranes. Supplementary material available online at

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Relationship between oxidizable fatty acid content and level of antioxidant glutathione peroxidases in marine fish

Grim

Hyndman

Kriska

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…A standard protocol was used for cDNA production with RevertAID reverse transcriptase and random nanomer primer from 1 μg of total RNA. The primers used for the quantification of the mRNA transcriptional levels of the target and endogenous control gene (β-actin) were chosen based on the literature (Hermesz and Ferencz, 2009;Jiang et al, 2015) and are shown in Table 2. Table 2 Primers of target (gpx4a and gpx4b) and endogenous control (β-actin) genes…”

Section: Rna Isolation Reverse Transcription and Qpcrmentioning

confidence: 99%

Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp

Pelyhe

Kövesi

Zándoki

et al. 2016

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

The purpose of this study was to investigate the short-term effects of a single oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg -1 body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg -1 body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours. T-2 toxin did not cause changes in these parameters. Glutathione content and glutathione peroxidase activity showed higher levels at the 16th hour as the effect of both mycotoxins. The expression of glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) revealed a dual response. Downregulation was observed at the 8th hour, followed by an induction at the 16th hour, at the lowest dose of both mycotoxins. Higher doses revealed long-drawn emergence and an elevation was observed only at the 24th hour. However, at the lowest and highest doses of DON or T-2 toxin the changes in gene expression were delayed, which may be related to the low oxidative stress response, as suggested by the expression profiles of the nrf2, keap1, gpx4a and gpx4b genes.

show abstract

“…Some antioxidant enzymes (e.g., GPx and GST) are polygenic. In common carp ( Cyprinus carpio ), GPx1 and GPx4 have been identified [12], and nine GST genes ( GST-alpha , -kappa , -theta , -mu , -pi , -rho , mGST1 , mGST2 and mGST3 ) have been cloned [13]. Despite being in the same family, genes encoding antioxidant enzymes tend to exhibit differing tissue distribution and function.…”

Section: Introductionmentioning

confidence: 99%

“…In river pufferfish ( Takifugu obscurus ), GST-theta , -mu , -MAPEG and -zeta gene expression in the liver were higher than GST-alpha , -kappa and -omega gene expression [14]. Among GPxs, GPx4 is the only one capable of reducing phospholipid hydroperoxides in vertebrate [12]. Furthermore, a recent study from our laboratory showed that antioxidant enzyme genes in the same family also have varied responses to nutrient content: dietary vitamin C deficiency downregulated gene expressions of GST-R1 , -P1 and -P2 , while not affecting gene expressions of GST-O1 and -O2 in the spleen of grass carp ( Ctenopharyngodon idella ) [15].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study on Antioxidant System in Fish Hepatopancreas and Intestine Affected by Choline Deficiency: Different Change Patterns of Varied Antioxidant Enzyme Genes and Nrf2 Signaling Factors

Liu

Jiang

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

The liver and intestine are susceptible to the oxidative damage which could result in several diseases. Choline deficiency induced oxidative damage in rat liver cells. Thus, this study aimed to investigate the potential molecular mechanisms responsible for choline deficiency-induced oxidative damage. Juvenile Jian carp were fed diets differing in choline content [165 (deficient group), 310, 607, 896, 1167 and 1820 mg/kg diet] respectively for 65 days. Oxidative damage, antioxidant enzyme activities and related gene expressions in the hepatopancreas and intestine were measured. Choline deficiency decreased choline and phosphatidylcholine contents, and induced oxidative damage in both organs, as evidenced by increased levels of oxidative-stress markers (malondialdehyde, protein carbonyl and 8-hydroxydeoxyguanosine), coupled with decreased activities of antioxidant enzymes [Copper-zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)]. However, choline deficiency increased glutathione contents in the hepatopancreas and intestine. Furthermore, dietary choline deficiency downregulated mRNA levels of MnSOD, GPx1b, GST-rho, mGST3 and Kelch-like ECH associating protein 1 (Keap1b) in the hepatopancreas, MnSOD, GPx1b, GPx4a, GPx4b, GST-rho, GST-theta, GST-mu, GST-alpha, GST-pi and GST-kappa in the intestine, as well as intestinal Nrf2 protein levels. In contrast, choline deficiency upregulated the mRNA levels of GPx4a, GPx4b, mGST1, mGST2, GST-theta, GST-mu, Keap1a and PKC in the hepatopancreas, mGST3, nuclear factor erythoid 2-related factor 2 (Nrf2) and Keap1a in the intestine, as well as hepatopancreatic Nrf2 protein levels. This study provides new evidence that choline deficiency-induced oxidative damage is associated with changes in the transcription of antioxidant enzyme and Nrf2/Keap1 signaling molecules in the hepatopancreas and intestine. Additionally, this study firstly indicated that choline deficiency induced varied change patterns of different GPx and GST isoforms. Meanwhile, the changes of some GPx and GST isoforms caused by choline deficiency in the intestine were contrary to those in the hepatopancreas.

show abstract

Identification of two phospholipid hydroperoxide glutathione peroxidase (gpx4) genes in common carp

Cited by 30 publications

References 30 publications

Relationship between oxidizable fatty acid content and level of antioxidant glutathione peroxidases in marine fish

Relationship between oxidizable fatty acid content and level of antioxidant glutathione peroxidases in marine fish

Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp

A Comparative Study on Antioxidant System in Fish Hepatopancreas and Intestine Affected by Choline Deficiency: Different Change Patterns of Varied Antioxidant Enzyme Genes and Nrf2 Signaling Factors

Contact Info

Product

Resources

About