Identification of Two Possible Types of Virus Particle in Rubella-infected Cells

Abstract

SUMMARYCultures of RK 13 and BHK2I ceils infected with rubella virus were examined by electron microscopy when the cultures showed maximal cytopathic effects. Infected RK 13 cells contained crystalline inclusions (spacing t9o X) as well as typical virus particles of total diameter 60o X, with a dense 300 ~, core. Identical particles also occurred in infected BHK2I ceils, but in these no crystals were observed. Neither crystals nor particles were found in control cells. The particles did not resemble myxoviruses.

Rubella Virus Replication and Links to Teratogenicity

“…RV maturation is a budding process in which the viral core acquires an envelope membrane after passing through modified host cell membranes (98). The Golgi apparatus, rough ER (RER), cytoplasmic vacuoles, and plasma mem-brane have been identified as sites of RV maturation (64,65,83). This is in contrast to the alphaviruses, where the plasma membrane is the only site of virus budding (159).…”

Intracellular transport of rubella virus structural proteins expressed from cloned cDNA

“…It is not clear why some cells should be more permissive of transport than others; however, it is not possible to ascribe the variant reports in the literature as to the site of RV budding to the same cause as our observations of cell line variation in transport of RV proteins. Of the four lines in our study, BHK and RK13 cells allowed the most transport of E1 and E2 to the surface, yet the latter cell line has been consistently reported as showing only intracellular budding (Hamvas et al, 1969;Higashi, 1973;Holmes et al, 1968;Patrizi & Middlekamp, 1970). On the other hand, RV-infected RK13 cells form syncytia, suggesting that the viral fusion protein, at least, is found at the cell surface (Patrizi & Middlekamp, 1970).…”

Growth of Rubella Virus in BHK 21 Cells: Electron Microscopy of Morphogenesis

“…Since the results by Best et al (I967), Holmes & Warburton (1967) and Holmes et aL (1968) describing enveloped, round particles, with a distinct nucleoid, about 60 nm. in diameter, several other workers have come to the same conclusions about the structure.…”