Identification of two small regulatory RNAs linked to virulence in <i>Brucella abortus</i> 2308

Caswell, Clayton C.; Gaines, Jennifer M.; Ciborowski, Paweł; Smith, Derek; Borchers, Christoph H.; Roux, Christelle M.; Sayood, Khalid; Dunman, Paul M.; Roop, R. Martin

doi:10.1111/j.1365-2958.2012.08117.x

Cited by 72 publications

(121 citation statements)

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“…These sRNAs include AbcR1 and AbcR2 (39), orthologues of SmrC15 and SmrC16 of Rhizobium etli, and AbcR1 and AbcR2 of Agrobacterium tumefaciens. These two sRNAs are required for full virulence of B. abortus 2308.…”

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confidence: 99%

Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing

et al. 2016

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Recent breakthroughs in next-generation sequencing technologies have led to the identification of small noncoding RNAs (sRNAs) as a new important class of regulatory molecules. In prokaryotes, sRNAs are often bound to the chaperone protein Hfq, which allows them to interact with their partner mRNA(s). We screened the genome of the zoonotic and human pathogen Brucella suis 1330 for the presence of this class of RNAs. We designed a coimmunoprecipitation strategy that relies on the use of Hfq as a bait to enrich the sample with sRNAs and eventually their target mRNAs. By deep sequencing analysis of the Hfq-bound transcripts, we identified a number of mRNAs and 33 sRNA candidates associated with Hfq. The expression of 10 sRNAs in the early stationary growth phase was experimentally confirmed by Northern blotting and/or reverse transcriptase PCR.

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Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing

et al. 2016

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“…This supports the finding that B. abortus VtlR transcription regulator target mRNAs (bab1-0310, bab1-0313, bab1-0314, and bab1-1794) are regulated by AbcR sRNAs and show higher regulatory gene expression with ∆AbcR sRNAs (38). In addition, AbcR sRNAs were reported to control the expression of Lrp-encoded gene BAB1-1587 in B. abortus, and expression decreased with ∆AbcR sRNAs; this finding highlights the importance of AbcR sRNA in the regulation of metabolism and amino acid transport (10). In contrast to the conserved region of AbcR1 sRNA, the regulation of target mRNA fusion was dependent on the seed pairing of AbcR1 sRNA.…”

Section: Discussionmentioning

confidence: 66%

“…They have been (ABC) transport systems, encoding genes in closely related bacteria, ie, B. abortus and A. tumefaciens. Additionally, they highlighted the role of AbcR sRNA in the virulence of B. abortus (10).…”

Section: Introductionmentioning

confidence: 99%

Small Noncoding RNA AbcR1 Addressing Multiple Target mRNAs From Transcriptional Factor and Two-Component Response Regulator of Brucella melitensis

Waqas

Zheng

et al. 2017

Jundishapur J Microbiol

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“…The detection of RNA transcripts was performed using a Northern blotting protocol outlined by Caswell et al (67). Briefly, RNA from cultures grown for 3 h was isolated using an RNeasy kit (Qiagen), with DNA removed using a Turbo DNA-free kit (Ambion).…”

Section: Methodsmentioning

confidence: 99%

The ω Subunit Governs RNA Polymerase Stability and Transcriptional Specificity in Staphylococcus aureus

et al. 2017

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Staphylococcus aureus is a major human pathogen that causes infection in a wide variety of sites within the human body. Its ability to adapt to the human host and to produce a successful infection requires precise orchestration of gene expression. While DNA-dependent RNA polymerase (RNAP) is generally well characterized, the roles of several small accessory subunits within the complex have yet to be fully explored. This is particularly true for the omega ( or RpoZ) subunit, which has been extensively studied in Gram-negative bacteria but largely neglected in Grampositive counterparts. In Escherichia coli, it has been shown that ppGpp binding, and thus control of the stringent response, is facilitated by . Interestingly, key residues that facilitate ppGpp binding by are not conserved in S. aureus, and consequently, survival under starvation conditions is unaffected by rpoZ deletion. Further to this, -lacking strains of S. aureus display structural changes in the RNAP complex, which result from increased degradation and misfolding of the ␤= subunit, alterations in ␦ and factor abundance, and a general dissociation of RNAP in the absence of . Through RNA sequencing analysis we detected a variety of transcriptional changes in the rpoZ-deficient strain, presumably as a response to the negative effects of depletion on the transcription machinery. These transcriptional changes translated to an impaired ability of the rpoZ mutant to resist stress and to fully form a biofilm. Collectively, our data underline, for the first time, the importance of for RNAP stability, function, and cellular physiology in S. aureus. IMPORTANCEIn order for bacteria to adjust to changing environments, such as within the host, the transcriptional process must be tightly controlled. Transcription is carried out by DNA-dependent RNA polymerase (RNAP). In addition to its major subunits (␣ 2 ␤␤=) a fifth, smaller subunit, , is present in all forms of life. Although this small subunit is well studied in eukaryotes and Gram-negative bacteria, only limited information is available for Gram-positive and pathogenic species. In this study, we investigated the structural and functional importance of , revealing key roles in subunit folding/stability, complex assembly, and maintenance of transcriptional integrity. Collectively, our data underline, for the first time, the importance of for RNAP function and cellular harmony in S. aureus.KEYWORDS RNA polymerase subunit omega, RpoZ, Staphylococcus aureus, gene regulation T ranscription in all forms of life is a tightly controlled process, necessitated by the essentiality of correct temporal and spatial expression of genes for survival. All transcriptional activity within a cell is maintained by the DNA-dependent RNA polymerase (RNAP). This multiprotein complex is structurally and functionally similar in distant forms of life, displaying only minor variations in composition, e.g., the presence/ absence of certain subunits (1, 2). In bacteria RNAP consists of four main subunits, i.e.,

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Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308

Cited by 72 publications

References 62 publications

Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing

Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing

Small Noncoding RNA AbcR1 Addressing Multiple Target mRNAs From Transcriptional Factor and Two-Component Response Regulator of Brucella melitensis

The ω Subunit Governs RNA Polymerase Stability and Transcriptional Specificity in Staphylococcus aureus

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