Identification of Two Subsets of Murine DC1 Dendritic Cells That Differ by Surface Phenotype, Gene Expression, and Function

Abstract

Classical dendritic cells (cDCs) in mice have been divided into 2 major subsets based on the expression of nuclear transcription factors: a CD8+Irf8+Batf3 dependent (DC1) subset, and a CD8-Irf4+ (DC2) subset. We found that the CD8+DC1 subset can be further divided into CD8+DC1a and CD8+DC1b subsets by differences in surface receptors, gene expression, and function. Whereas all 3 DC subsets can act alone to induce potent Th1 cytokine responses to class I and II MHC restricted peptides derived from ovalbumin (OVA) by OT-I and OT-II transgenic T cells, only the DC1b subset could effectively present glycolipid antigens to natural killer T (NKT) cells. Vaccination with OVA protein pulsed DC1b and DC2 cells were more effective in reducing the growth of the B16-OVA melanoma as compared to pulsed DC1a cells in wild type mice. In conclusion, the Batf3-/- dependent DC1 cells can be further divided into two subsets with different immune functional profiles in vitro and in vivo.

Single‐cell RNA sequencing distinguishes dendritic cell subsets in the rat, allowing advanced characterization of the effects of FMS‐like tyrosine kinase 3 ligand

“…We identified cDC1, cDC2, and pDC clusters (Figure 2A) based on unique subset‐associated transcriptional programming (Figure 2B). The transcript expression levels for lineage‐identifying genes are shown for cDC1 (Figure 2C), cDC2 (Figure 2D), and pDC (Figure 2E) which are homologous to those found in murine and human models 36‐38 . As expected, cDC1 express X‐C motif chemokine receptor 1 (XCR1) and interferon regulatory factor (IRF) 8, cDC2 express signal regulatory protein α (SIRPα) and IRF4, and pDC express siglec‐H and TLR7.…”

“…The transcript expression levels for lineage-identifying genes are shown for cDC1 (Figure 2C), cDC2 (Figure 2D), and pDC (Figure 2E) which are homologous to those found in murine and human models. [36][37][38] As expected, cDC1 express X-C motif chemokine receptor 1 (XCR1) and interferon regulatory factor (IRF) 8, cDC2 express signal regulatory protein α (SIRPα) and IRF4, and pDC express siglec-H and TLR7. While these markers robustly identify cDC1, cDC2, and pDC on the gene level, most are not commercially available as antibodies reactive with the rat model and are thus not suitable for subset identification by flow cytometry.…”

GPS2-mediated regulation of the adipocyte secretome modulates adipose tissue remodeling at the onset of diet-induced obesity

“…4D ) [ 7 , 26 , 69 , 103 , 145 , 147 ]; (iii) the expression of specific DC markers via MFI quantification ( Supplemental Fig. 4E ) [ 13 , 31 , 71 , 161 , 175 ].…”

An association between elevated telomerase reverse transcriptase expression and the immune tolerance disruption of dendritic cells

“…DC1s [X-C Motif Chemokine Receptor 1 (XCR1) + CD11c + ; also called myeloid DCs] produce IL-12, and initiate T helper (Th)1 response. DC2s (also called lymphoid DCs; CD103 + CD172a + ) initiate Th2 response [ 1 – 3 ]. Tolerogenic DCs (TolDCs) are a subtype of DCs, CD141 + [ 1 – 3 ].…”

“…DC2s (also called lymphoid DCs; CD103 + CD172a + ) initiate Th2 response [ 1 – 3 ]. Tolerogenic DCs (TolDCs) are a subtype of DCs, CD141 + [ 1 – 3 ]. Semi-maturation is the feature of TolDCs based on the low expression MHC II, CD80, CD86.…”