Identification of unexplored substrates of the serine protease, thrombin, using N-terminomics strategy

Bhagwat, Sonali R.; Hajela, Krishnan; Bhutada, Sumit; Choudhary, Komal; Sharma, S. N.; Kumar, Amit

doi:10.1016/j.ijbiomac.2019.12.137

Cited by 8 publications

(4 citation statements)

References 75 publications

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“…Bhagwat et al used LC-MS/MS to identify thrombin substrates. 154 Whereas this is an n = 1 study, it provides a proof of principle for future studies in this space, identifying 54 substrates for thrombin and opening the door for studies in clinical settings that can identify whether the thrombin substrates change depending on the health status, age, and so on of individuals. Using LC-MS/ MS, Stachowicz et al 155 investigated the differences in the ex vivo plasma clot composition in patients with thrombotic antiphospholipid syndrome (APS) compared with VTE, identifying differences in up to 63 proteins.…”

Section: ■ Hemostasismentioning

confidence: 99%

“…Thrombin is known as the “master regulator” protein in hemostasis, playing a key role in the fine balance between bleeding and clotting. Bhagwat et al used LC-MS/MS to identify thrombin substrates . Whereas this is an n = 1 study, it provides a proof of principle for future studies in this space, identifying 54 substrates for thrombin and opening the door for studies in clinical settings that can identify whether the thrombin substrates change depending on the health status, age, and so on of individuals.…”

Section: Hemostasismentioning

confidence: 99%

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Advances and Utility of the Human Plasma Proteome

et al. 2021

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The study of proteins circulating in blood offers tremendous opportunities to diagnose, stratify, or possibly prevent diseases. With recent technological advances and the urgent need to understand the effects of COVID-19, the proteomic analysis of blood-derived serum and plasma has become even more important for studying human biology and pathophysiology. Here we provide views and perspectives about technological developments and possible clinical applications that use mass-spectrometry(MS)- or affinity-based methods. We discuss examples where plasma proteomics contributed valuable insights into SARS-CoV-2 infections, aging, and hemostasis and the opportunities offered by combining proteomics with genetic data. As a contribution to the Human Proteome Organization (HUPO) Human Plasma Proteome Project (HPPP), we present the Human Plasma PeptideAtlas build 2021-07 that comprises 4395 canonical and 1482 additional nonredundant human proteins detected in 240 MS-based experiments. In addition, we report the new Human Extracellular Vesicle PeptideAtlas 2021-06, which comprises five studies and 2757 canonical proteins detected in extracellular vesicles circulating in blood, of which 74% (2047) are in common with the plasma PeptideAtlas. Our overview summarizes the recent advances, impactful applications, and ongoing challenges for translating plasma proteomics into utility for precision medicine.

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Section: ■ Hemostasismentioning

confidence: 99%

Section: Hemostasismentioning

confidence: 99%

Advances and Utility of the Human Plasma Proteome

et al. 2021

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“…Proteomics studies have identified 54 different substrates of thrombin suggesting the need for future clinical studies of specific thrombin substrates in individuals based on age or health status. 14 Venous Thromboembolism VTE affects 10 million individuals globally each year and the need for novel predictive biomarkers of VTE is imperative. 15 Two studies from the past 10 years utilized proteomics to identify novel biomarkers associated with VTE incidence in adults.…”

Section: Blood Clot Compositionmentioning

confidence: 99%

Section: Blood Clot Compositionmentioning

confidence: 99%