The validated influence of urban biophysical structure on environmental processes within urban areas has heightened the emphasis on studies examining morphological patterns to determine precise locations and underlying causes of urban climate conditions. The present study aims to characterise morphological patterns describing the distribution of Land Surface Temperature (LST) based on a prior classification of biophysical variables, including urban density (building intensity and average height), surface characteristics, shortwave solar radiation (broadband albedo), and seasonal variations in vegetation cover (high, medium, and low levels), retrieved from multisource datasets. To describe the distribution of LST, the variables were calculated, classified, and subsequently, analysed individually and collectively concerning winter and summer LST values applied in an urban neighbourhood in Madrid, Spain. The results from the analytical approaches (observation, correlations, and multiple regressions) were compared to define the morphological patterns. The selection of areas resulting from the morphological patterns with the most unfavourable LST values showed agreement of up to 89% in summer and up to 70% for winter, demonstrating the feasibility of the methods applied to identify priority areas for intervention by season. Notably, low and high vegetation levels emerged as pivotal biophysical characteristics influencing LST distribution compared to the other characteristics, emphasising the significance of integrating detailed seasonal vegetation variations in urban analyses.