Identification of urinary metabolites with potential blood pressure-lowering effects in lentil-fed spontaneously hypertensive rats

Abstract

Consumption of lentils increases the availability of arginine and several related compounds that could potentially elevate production of NO and contribute to the blood pressure-lowering effects of lentil-rich diets.

Investigation of low molecular weight peptides (<1 kDa) in chicken meat and their contribution to meat flavor formation

“…Cytidine (C 9 H 13 N 3 O 5 ) and norvaline (C 5 H 11 NO 2 ) were used as internal standards (IS) to validate the extraction and MS methods used in this study. These compounds were chosen because their efficacy has been previously tested in our laboratory when added as IS to either biological samples or food samples . Cytidine gave satisfactory results in our previous HPLC analysis of nucleotides, nucleosides and bases in chicken breast and bison meats coupled with UV detection and was selected to be tested in this LC/QTOF‐MS application.…”

Discovery of Intake Biomarkers of Lentils, Chickpeas, and White Beans by Untargeted LC–MS Metabolomics in Serum and Urine

“…Maximum excretions of these compounds were observed 4 and 6 h, respectively, after intake of lentils, although differences were observed from the first few hours until 48 h post-consumption (Table 1). Hydroxyarginine is a free nonprotein amino acid particular to lentils, [9] and oxoarginine is a metabolite of the arginine pathway recently associated with lentil consumption. [9] Therefore, they are interesting indicators of lentil intake.…”

“…Hydroxyarginine is a free nonprotein amino acid particular to lentils, [9] and oxoarginine is a metabolite of the arginine pathway recently associated with lentil consumption. [9] Therefore, they are interesting indicators of lentil intake. Additionally, it has been suggested that they could contribute to the blood pressure-lowering effects associated with the consumption of lentils through an increase in the production of nitric oxide.…”

“…[5] To date, only a limited number of foods have been associated with validated BFIs, but pulses are not among them, since only a few studies dealing with this topic have been recently published. [6][7][8][9][10][11] The first of them was focused on the quantification of kaempferol in urine after bean consumption. [6] However, this compound is present in a wide range of vegetables.…”

“…[7] Animal studies reported an increase in the urinary excretion of trigonelline, homoeriodictyol chalcone, and two peptides after a bean-based diet in dogs, [8] and increased urinary excretions of different species of dipeptides, as well as arginine-related metabolites in hypertensive rats fed with lentils. [9] More recently our research group published two human studies applying untargeted NMR experiments, one in an observational framework [10] while the other was a postprandial controlled intervention study. [11] The first study showed that pulse consumers had higher urinary excretions of different metabolites related to choline, protein, and energy metabolism, [10] whereas the results of the second study suggested trigonelline, 3-methylhistidine, dimethylglycine, TMA, glutamine, choline, lysine, and histidine as candidate biomarkers of pulse intake.…”