Identification of V3 Loop-binding Proteins as Potential Receptors Implicated in the Binding of HIV Particles to CD4+Cells

Callebaut, Christian; Blanco, Julià; Benkirane, Nadia; Krust, Bernard; Jacotot, Étienne; Guichard, Gilles; Seddiki, Nabila; Svab, Josette; Dam, Elisabeth; Muller, Sylviane; Briand, Jean‐Paul; Hovanessian, Ara G.

doi:10.1074/jbc.273.34.21988

Cited by 73 publications

(134 citation statements)

References 62 publications

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“…The specificity of the 125 I-labeled MK binding to the low affinity binding site was confirmed by the fact that unlabeled MK prevented this binding. The nucleolin-binding HB-19 pseudopeptide [1,14,15] drastically inhibited the 125 I-labeled MK binding to the low affinity site. On the other hand, the N-terminal tail peptide of MK or the C-terminal tail peptide of PTN had no apparent effect.…”

Section: The Nucleolin-binding Hb-19 Pseudopeptide Blocks the Bindingmentioning

confidence: 98%

“…The expression of the nucleolin at the cell surface was illustrated by using a specific antagonist of nucleolin, the HB-19 pseudopeptide that was designed initially as a potent inhibitor of HIV infection [13][14][15][16]. HB-19 presents pentavalently the tripeptide KPR in which the peptide bond between the K and P is reduced in order to increase the endopeptidase resistance of this pseudopeptide.…”

Section: Expression Of Nucleolin At the Cell Surfacementioning

confidence: 99%

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Midkine, a cytokine that inhibits HIV infection by binding to the cell surface expressed nucleolin

Hovanessian

2006

Cell Res

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The growth factor midkine (MK) is a cytokine that inhibits HIV infection in cell cultures in an autocrine and paracrine manner by blocking the attachment of HIV particles to permissive cells. MK mRNA is systematically expressed in adult peripheral blood lymphocytes from healthy donors, while its expression becomes markedly but transiently increased upon in vitro treatment of lymphocytes with IL-2 or IFN-g and activation of T lymphocytes by PHA or through the engagement of the CD28 antigen. The binding of MK to cells occurs specifically at a high and a low affinity binding site. This low affinity-binding site is the cell-surface expressed nucleolin, which is implicated in the mechanism of the initial attachment of HIV particles to cells. Accordingly, the nucleolin-binding HB-19 pseudopeptide has no effect on the MK binding to the high affinity binding site, whereas it prevents the binding of MK to the low affinity binding site, thus suggesting the low affinity receptor of MK is the cell-surface-expressed nucleolin. Confocal immunofluorescence laser microscopy revealed the colocalization of MK and the cell-surface-expressed nucleolin at distinct spots. The use of various deletion constructs of nucleolin then indicates that the extreme C-terminal end of nucleolin, containing repeats of the amino acid motif RGG, as the domain that binds MK. The specific binding of MK to the surface nucleolin is independent of heparan sulfate and chondroitin sulfate proteoglycans. After binding to cells, MK enters cells by an active process in which nucleolin and lipid rafts appear to be implicated. The potent and the distinct anti-HIV action of MK along with its enhanced expression in lymphocytes by various physiological stimuli, point out that MK is a cytokine that could be involved in HIV pathogenesis.

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Section: The Nucleolin-binding Hb-19 Pseudopeptide Blocks the Bindingmentioning

confidence: 98%

Section: Expression Of Nucleolin At the Cell Surfacementioning

confidence: 99%

Midkine, a cytokine that inhibits HIV infection by binding to the cell surface expressed nucleolin

Hovanessian

2006

Cell Res

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“…The purified peptide-complexed proteins were denatured by heating in the electrophoresis sample buffer containing SDS and analyzed by SDS͞PAGE electrophoresis (14). The presence of nucleolin was then revealed by immunoblotting using rabbit polyclonal antibody against hamster (CHO cells) nucleolin as described (14). Similarly purified peptide-complexed proteins were eluted by 1 M NaCl and dialyzed against PBS containing 1 mM EDTA and protease inhibitor mixture.…”

Section: Characterization Of Tissue Nucleolin Complexed With the Biotin-mentioning

confidence: 99%

“…These experimental conditions were previously optimized for the recovery of the cell-surface-expressed nucleolin complexed with the biotin-labeled HB-19 in vitro (7,13,14). Anesthetized rats were injected intravenously with 1 mg of the biotin-labeled HB-19 or the biotin-labeled control peptide CP-51.…”

Section: Characterization Of Tissue Nucleolin Complexed With the Biotin-mentioning

confidence: 99%

“…After 2 h of incubation at 4°C, the samples were washed extensively with PBS-EDTA. The purified peptide-complexed proteins were denatured by heating in the electrophoresis sample buffer containing SDS and analyzed by SDS͞PAGE electrophoresis (14). The presence of nucleolin was then revealed by immunoblotting using rabbit polyclonal antibody against hamster (CHO cells) nucleolin as described (14).…”

Section: Characterization Of Tissue Nucleolin Complexed With the Biotin-mentioning

confidence: 99%

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The anti-HIV pentameric pseudopeptide HB-19 is preferentially taken up in vivo by lymphoid organs where it forms a complex with nucleolin

Krust

Vienet

Cardona

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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Survey of the 1998 optical biosensor literature

Myszka

1999

J. Mol. Recognit.

120

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The utilization of optical biosensors to study molecular interactions continues to expand. In 1998, 384 articles relating to the use of commercial biosensors were published in 130 different journals. While significant strides in new applications and methodology were made, a majority of the biosensor literature is of rather poor quality. Basic information about experimental conditions is often not presented and many publications fail to display the experimental data, bringing into question the credibility of the results. This review provides suggestions on how to collect, analyze and report biosensor data.

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Identification of V3 Loop-binding Proteins as Potential Receptors Implicated in the Binding of HIV Particles to CD4+Cells

Cited by 73 publications

References 62 publications

Midkine, a cytokine that inhibits HIV infection by binding to the cell surface expressed nucleolin

Midkine, a cytokine that inhibits HIV infection by binding to the cell surface expressed nucleolin

The anti-HIV pentameric pseudopeptide HB-19 is preferentially taken up in vivo by lymphoid organs where it forms a complex with nucleolin

Survey of the 1998 optical biosensor literature

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