Identification of vitamin B12 analogues by liquid chromatography with electrothermal atomic absorption detection

Viñas, Pilar; Campillo, Natalia; López-Garcı́a, Ignacio; Hernández‐Córdoba, Manuel

doi:10.1007/bf02290293

Cited by 19 publications

(11 citation statements)

References 17 publications

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“…4 More specific but cumbersome and time-consuming alternatives include the classical microbiological assay using Lactobacillus leichmannii 5 and a radioimmunoassay which requires a radioisotopelabelled vitamin and a specific binder. 6 Reversed-phase HPLC methods with UV, 7,8 spectrophotometric, [9][10][11] atomic absorption spectrometric (AAS) 12,13 and inductively coupled plasma atomic emission spectrometric (ICP-AES) detection 14 allow species-selective quantification of several cobalamins and their analogues at the mg ml 21 level during a 30 min run.…”

mentioning

confidence: 99%

Direct species-selective determination of cobalamins by ionspray mass spectrometry and ionspray tandem mass spectrometry

Chassaigne¹,

Łobiński²

1998

Analyst

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A direct method for the simultaneous compound-specific determination of vitamin B 12 (cyanocobalamin), its analogues methylcobalamin, hydroxocobalamin, adenosylcobalamin (coenzyme B 12 ) and cobinamides by ionspray MS was developed. Single and tandem MS modes were compared. For all the compounds except hydroxocobalamin, the most intense ion formation was observed as a result of protonation of the original molecular cation leading to an analytically useful doubly charged ion. For hydroxocobalamin a similar process leads to the loss of the functional group leading to the [Cbl + H] 2+ ion, which is analytically useful only in the absence of other cobalamins. Hydroxocobalamin can be determined in the presence of other compounds using the minor [OH-Cbl] + ion. The calibration curves are linear in the range 0.01-1 mg ml 21 , which can be extended up to 10 mg ml 21 if alternative ions are chosen. Detection limits of 5-40 ng ml 21 are obtained. Collision-induced dissociation leads first to the loss of the Co substituent (Me, CN, OH, adenosyl) and thus to a decrease in selectivity. The method was applied to the analysis of a synthetic mixture of the cobalamins and to the determination of hydroxocobalamin in a commercial pharmaceutical preparation.

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confidence: 99%

Direct species-selective determination of cobalamins by ionspray mass spectrometry and ionspray tandem mass spectrometry

Chassaigne¹,

Łobiński²

1998

Analyst

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“…79,80,82,83,85,86,158,[165][166][167] With an autosampler and flow-through cells a high degree of automation can be achieved, leading to a quasi-on-line coupling. Off-line analysis of MT-bound metals after HPLC by fraction collection and ETAAS analysis has been a common approach.…”

Section: Element-specific Detection In Hplcmentioning

confidence: 99%

Bio-inorganic speciation analysis by hyphenated techniques

Szpunar

2000

Analyst

279

197

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International audienceThe state-of-the-art of species-selective analysis for trace metals and metalloids in biological materials by chromatographic and electrophoretic separation techniques with element selective detection is critically reviewed. The species of interest include organoarsenic, organoselenium and metal complexes with bioglands such as phytochelatins, metallothioneins, proteins and polysaccharides. The separation mechanisms discussed include size-exclusion, anion- and cation-exchange and reversed-phase HPLC and flatbed and capillary zone electrophoresis. Advantages and limitations of various element selective (e.g. AAS, ICP-AES and ICP-MS) and molecule specific (electrospray MS/MS) detection techniques used on- and off-line are discussed. The applications of coupled techniques to the analysis of biological materials are comprehensively reviewed in tabular form. Attention is paid to the sample preparation and sources of error in bioinorganic speciation analysis

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“…Also MBA lacks a high degree of specicity as food commodities can contain inactive cobalamins that can interfere with the growth of the microorganism. Other methods include radioisotope dilution assay, 10 atomic absorption spectrometry (AAS), 11,12 inductively coupled plasma mass spectrometry (ICP-MS), 13 capillary electrophoresis (CE), 14 chemiluminescence techniques, 15 biomolecular interaction analysis (BIA) 16 and a combination of uorescence resonance energy transfer (FRET) and ow-injection analysis (FIA). 17 Liquid chromatography has recently emerged as an efficient technique for determination of vitamin B 12 .…”

Section: Introductionmentioning

confidence: 99%

A simple and sensitive LC-ICP-MS method for the accurate determination of vitamin B12 in fortified breakfast cereals and multivitamin tablets

Raju

Schiel

et al. 2013

J. Anal. At. Spectrom.

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Identification of vitamin B12 analogues by liquid chromatography with electrothermal atomic absorption detection

Cited by 19 publications

References 17 publications

Direct species-selective determination of cobalamins by ionspray mass spectrometry and ionspray tandem mass spectrometry

Direct species-selective determination of cobalamins by ionspray mass spectrometry and ionspray tandem mass spectrometry

Bio-inorganic speciation analysis by hyphenated techniques

A simple and sensitive LC-ICP-MS method for the accurate determination of vitamin B12 in fortified breakfast cereals and multivitamin tablets

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