Identification of volatile markers for indoor fungal growth and chemotaxonomic classification of Aspergillus species

Polizzi, Viviana; Adams, An; Malysheva, Svetlana V.; Saeger, Sarah De; Peteghem, Carlos Van; Moretti, Antonio; Picco, Anna Maria; Kimpe, Norbert De

doi:10.1016/j.funbio.2012.06.001

Cited by 76 publications

(55 citation statements)

References 42 publications

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“…In general, the profiles of the volatiles identified here were differentiated both within strains of the same species, as well as between species. This finding is consistent with the observations from approximated studies on the chemotaxonomic classification of Penicillium, Aspergillus, and other fungal species (Fiedler et al 2001;Sunesson et al 1995;Polizzi et al 2012).…”

Section: Discussionsupporting

confidence: 92%

Formation of 6-n-pentyl-2H-pyran-2-one (6-PAP) and other volatiles by different Trichoderma species

et al. 2013

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Section: Discussionsupporting

confidence: 92%

Formation of 6-n-pentyl-2H-pyran-2-one (6-PAP) and other volatiles by different Trichoderma species

et al. 2013

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“…Considering the MVOC production of a well-known species belonged to the same group, A. versicolor, A. creber resulted similar for the emission of 1,3-pentadiene, 1-octene, 3-methyl-1-butanol, 2-octene-1-ol, 3-octanone and 3-methylfuran (Fiedler et al, 2001;Polizzi et al, 2012;Schuchardt and Kruse, 2009;Sunesson et al, 1995). As reported by Matysik et al (2008) for A. versicolor, the temporary trend of the main emission by A. creber recorded a maximum peak around 6 days after incubation, followed by a general decrease.…”

Section: Discussionmentioning

confidence: 92%

“…1-octen-3-ol and 3-octanol, as well as ketones and furans were addressed as indicators of mould, recognised both on pure culture studied, on agar substrate, and on wallpaper and building materials (Polizzi et al, 2012). Aspergillus and Penicillium are the most investigated fungal genera in MVOC studies (Fiedler et al, 2001;Matysik et al, 2008Matysik et al, , 2009Moularat et al, 2008;Polizzi et al, 2012;Schuchardt and Kruse, 2009;Wady et al, 2003, Wady andLarsson, 2005) because of their ubiquity in indoor environments (Cabral, 2010;Samson et al, 2004), also in association with the biodeterioration of Cultural Heritage (Micheluz et al, 2015;Sterflinger, 2010;Zyska, 1997). However, in the recent years, specific fungal contamination emerged inside Italian archives and library, mainly caused by a xerophilic fungus with a lack of knowledge about its MVOC emission capability, i.e.…”

Section: Introductionmentioning

confidence: 99%

Detection of volatile metabolites of moulds isolated from a contaminated library

Micheluz

Manente

Rovea

et al. 2016

Journal of Microbiological Methods

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The principal fungal species isolated from a contaminated library environment were tested for their microbial volatile organic compound (MVOC) production ability. Aspergillus creber, A. penicillioides, Cladosporium cladosporioides, Eurotium chevalieri, E. halophilicum, Penicillium brevicompactum and P. chrysogenum were cultivated on suitable culture media inside sample bottles specifically designed and created for direct MVOC injection to a GC-MS instrument. The fungal emissions were monitored over several weeks to detect changes with the aging of the colonies, monitored also by respirometric tests. A total of 55 different MVOCs were detected and isopropyl alcohol, 3-methyl-1-butanol and 2-butanone were the principal compounds in common between the selected fungal species. Moreover, 2,4-dimethylheptane, 1,4-pentadiene, styrene, ethanol, 2-methyl-1-butanol, acetone, furan and 2-methylfuran were the most detected compounds. For the first time, the MVOC production for particular fungal species was detected. The species A. creber, which belongs to the recently revised group Aspergillus section Versicolores, was characterized by the production of ethanol, furan and 1,4-pentadiene. For the xerophilic fungus E. halophilicum, specific production of acetone, 2-butanone and 1,4-pentadiene was detected, supported also by respirometric data. The results demonstrated the potential use of this method for the detection of fungal contamination phenomena inside Cultural Heritage's preservation environments.

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“…These volatile metabolites are produced during both primary and secondary metabolism and are often collectively referred to as microbial volatile organic compounds (MVOCs) [3]. MVOCs are widely investigated as the indicator of fungal growth [4][5][6][7], mycotoxins' production [8][9][10] and for fungal taxonomy [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…MVDA includes multivariate ANOVA (MANOVA), linear discriminant analysis (LDA), cluster analysis (CA), principal component analysis (PCA) and partial least square analysis (PLS). These methods are widely used in fungal detection and classification [11,[51][52][53].…”

Section: Introductionmentioning

confidence: 99%

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

et al. 2016

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Aspergillus flavus produces dangerous secondary metabolites known as aflatoxins, which are toxic and carcinogenic, and their contamination of agricultural products results in health issues and economic hardships in the U.S. and around the world. Early identification of aflatoxigenic isolates of A. flavus is the key in the management of these fungi. An emerging detection method for specific fungi identification involves the analysis of microbial volatile organic compounds (MVOCs) released by the fungi. Complicating this approach is the understanding that many factors influence metabolic production, including growth parameters, such as growth media, temperature, spore counts and oxidation stress. In addition, analytical and data analysis methods can also influence the results. Several growth and analysis methods were evaluated and optimized in order to better understand the effect of the methods on fungi MVOC signatures. The results indicate that carboxen/polydimethylsiloxane (CAR/PDMS) has the best extraction efficiency for the MVOCs emitted by A. flavus. Both chemical defined agar (CDA) and chemical defined liquid (CDL) are suitable growth media for MVOC emission studies. The highest MVOC production was found at 30˝C. Log transformation was considered one of the best data pretreatment methods when analyzing MVOC data and resulted in the best principal component analysis (PCA) clustering in the experiments with different growth media. This study aims to elucidate fungal volatile organic compounds (VOCs) differences due to variations in growth parameters as a first step in the development of an analytical method for the monitoring of aflatoxigenic A. flavus contamination in crop storage facilities.

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Identification of volatile markers for indoor fungal growth and chemotaxonomic classification of Aspergillus species

Cited by 76 publications

References 42 publications

Formation of 6-n-pentyl-2H-pyran-2-one (6-PAP) and other volatiles by different Trichoderma species

Formation of 6-n-pentyl-2H-pyran-2-one (6-PAP) and other volatiles by different Trichoderma species

Detection of volatile metabolites of moulds isolated from a contaminated library

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

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