Identification of Water Deficit Stress Upregulated Genes in Sugarcane

Gajjeraman, Prabu; Kawar, Prashant G.; Pagariya, Madhuri Chandrakant; Prasad, D.

doi:10.1007/s11105-010-0230-0

Cited by 90 publications

(54 citation statements)

References 58 publications

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“…Among these environmental factors, water deficiency and salinity are the major abiotic factors limiting sugarcane production (Menossi et al 2008), demanding efforts to investigate the adaptation and tolerance mechanisms of stress at the molecular level. In our earlier study, a suppression subtractive hybridization (SSH) approach was used to isolate ESTs upregulated during water-deficit stress in sugarcane and elucidate their transcriptional regulatory mechanisms (Prabu et al 2011). Among the genes identified, a waterdeficit stress-specific cDNA, ScMYBAS1 (accession no.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones

Gajjeraman

Prasad

2011

Plant Cell Rep

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The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) flanking the 5' ScMYBAS1 coding region from the sugarcane genome. A series of PScMYBAS1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -843, -1,033) was fused to the uidA reporter gene (GUS) and each deletion construct was analyzed by Agrobacterium-mediated transient transformation in tobacco leaves subjected to dehydration, salinity, cold, wounding, gibberellic acid (GA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Deletion analysis of the promoter, PScMYBAS1, suggested that the 303-bp promoter region was required for basal expression. Promoter fragments, 777 bp or longer showed ~twofold to ~fourfold increased induction of GUS in response to abiotic stress (dehydration, salt, cold, wounding) and hormone (SA, MeJA) treatments. These findings further our understanding of the regulation of ScMYBAS1 expression and provide a new stress-inducible promoter system in transgenic plants.

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Section: Introductionmentioning

confidence: 99%

Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones

Gajjeraman

Prasad

2011

Plant Cell Rep

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“…The SSH technique is believed to generate an equalized representation of differentially expressed genes and provides a high enrichment of differentially expressed mRNA (Diatchenko et al 1996;Marenda et al 2004). The efficiency and reproducibility of SSH are very useful in studies of tissue-specific, developmental, or induced differentially expressed genes (Von Stein et al 1997;Basyuni et al 2011;Prabu et al 2011;Yang et al 2011b). In addition, in some plant species, SSH has proved useful for identifying genes differentially expressed during zygotic and somatic embryogenesis (Bishop-Hurley et al 2003;Namasivayam and Hanke 2006;Legrand et al 2007;Tsuwamoto et al 2007;Wang et al 2007;Geng et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification and Analysis of Differentially Expressed Genes During Seed Development Using Suppression Subtractive Hybridization (SSH) in Phaseolus vulgaris

et al. 2011

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Interspecific hybridization in the genus Phaseolus, conducted to introgress desired traits into common bean (Phaseolus vulgaris L.), leads to the abortion of immature embryos, usually at early developmental stages. Little is known about the physiological responses of embryo dysfunction in P. vulgaris during the early stages of embryogenesis and the genes that are involved in these responses. Identification of the genes involved in Phaseolus embryogenesis may provide information that will help to understand the molecular basis of Phaseolus embryo dysfunction. To investigate the genes expressed during Phaseolus seed development, we constructed a suppression subtractive hybridization (SSH) library using cDNA from abortive seed development as the driver and those from normal seed development as tester. The differentially expressed cDNA fragments were identified by differential screening. We identified 72 unique ESTs of which we selected 12 candidates on the basis of their redundancy. These candidates were subjected to a validation procedure based on the study of their expression level by real-time PCR. Sequence analysis revealed that most of the differentially expressed genes are related to metabolism and regulation such as protein synthesis. Some genes also encoded transcription factors. These genes showed high mRNA transcript levels in seed tissues and little or no expression in other tissues (root, stem, flower, leaf, and cotyledon). Seven genes were chosen and their expression profile during seed development in P. vulgaris was analyzed by real-time PCR using RNA preparations originating from different seed development stages. This study revealed hitherto unknown genes putatively involved in dicotyledonous embryogenesis, which serve as a starting point for understanding Phaseolus embryogenesis.

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“…Therefore, the abscisic acid (ABA) (Kholová et al, 2010), the pH (Schachtman & Goodger, 2008) and the ionic distribution (Bahrun et al, 2002) seem to play an important role on signaling throughout the plant under water stress. Molecular studies have identified a wide range of genes expressed by sugarcane plants under water stress conditions (Iskandar et al, 2011;Prabu et al, 2010;Rodrigues et al, 2011). Regarding the responses to stress, signaling pathways regulated by hormones are highly drought-responsive, mainly those associated with the increased ABA synthesis (Pinheiro & Chaves, 2011).…”

Section: Physiological Parameters In Sugarcanementioning

confidence: 99%